TWIST2 (NM_057179) Human Mass Spec Standard

CAT#: PH305006

TWIST2 MS Standard C13 and N15-labeled recombinant protein (NP_476527)

Product Images

Coomassie blue staining of purified TWIST2 protein (Cat# TP305006). The protein was produced from HEK293T cells transfected with TWIST2 cDNA clone (Cat# RC205006) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205006
• Predicted MW: 18.1 kDa
• Protein Sequence:
  Sequence Link

  >RC205006 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRIL
  ANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHER
  LSYAFSVWRMEGAWSMSASH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_476527
• RefSeq Size: 1186
• RefSeq ORF: 480
• Synonyms: AMS; BBRSAY; bHLHa39; DERMO1; FFDD3; SETLSS
• Locus ID: 117581
• UniProt ID: Q8WVJ9, A1MB48
• Cytogenetics: 2q37.3
• Summary: The protein encoded by this gene is a basic helix-loop-helix type transcription factor and shares similarity with Twist. This protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype during osteoblast development. This gene may be upregulated in certain cancers. Mutations in this gene cause focal facial dermal dysplasia 3, Setleis type. Two transcript variants encoding the same protein have been found. [provided by RefSeq, Apr 2014]
• Protein Families: Druggable Genome, Transcription Factors