HSPC014 (POMP) (NM_015932) Human Mass Spec Standard

CAT#: PH304812

POMP MS Standard C13 and N15-labeled recombinant protein (NP_057016)

Product Images

Coomassie blue staining of purified POMP protein (Cat# TP304812). The protein was produced from HEK293T cells transfected with POMP cDNA clone (Cat# RC204812) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204812
• Predicted MW: 15.8 kDa
• Protein Sequence:
  Sequence Link

  >RC204812 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNARGLGSELKDSIPVTELSASGPFESHDLLRKGFSCVKNELLPSHPLELSEKNFQLNQDKMNFSTLRNI
  QGLFAPLKLQMEFKAVQQVQRLPFLSSSNLSLDVLRGNDETIGFEDILNDPSQSEVMGEPHLMVEYKLGL
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057016
• RefSeq Size: 1477
• RefSeq ORF: 423
• Synonyms: C13orf12; HSPC014; PNAS-110; PRAAS2; UMP1
• Locus ID: 51371
• UniProt ID: Q9Y244
• Cytogenetics: 13q12.3
• Summary: The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.[provided by RefSeq, Aug 2010]
• Protein Pathways: Proteasome