PYCR3 (NM_023078) Human Mass Spec Standard

CAT#: PH303382

PYCRL MS Standard C13 and N15-labeled recombinant protein (NP_075566)

Product Images

Coomassie blue staining of purified PYCR3 protein (Cat# TP303382). The protein was produced from HEK293T cells transfected with PYCR3 cDNA clone (Cat# RC203382) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203382
• Predicted MW: 28.6 kDa
• Protein Sequence:
  Sequence Link

  >RC203382 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCL
  LVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMAR
  GRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAA
  QTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_075566
• RefSeq Size: 2678
• RefSeq ORF: 822
• Synonyms: PYCRL
• Locus ID: 65263
• UniProt ID: Q53H96, A0A0A0MQS1
• Cytogenetics: 8q24.3
• Summary: This gene encodes a protein that belongs to the pyrroline-5-carboxylate reductase family of enzymes. Members of this family catalyze the final step in proline biosynthesis, converting pyrroline-5-carboxylate to proline. Glutamate and ornithine are precursors in the synthesis of proline. The protein encoded by this gene is a cytoplasmic enzyme involved in the biosynthesis of proline from ornithine. [provided by RefSeq, Aug 2016]
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways