MRPS25 (NM_022497) Human Mass Spec Standard

SKU
PH301353
MRPS25 MS Standard C13 and N15-labeled recombinant protein (NP_071942)
  $3,360.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC201353
Predicted MW 20.1 kDa
Protein Sequence
Protein Sequence
>RC201353 protein sequence
Red=Cloning site Green=Tags(s)

MPMKGRFPIRRTLQYLSQGNVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNM
TPSPFLRFYLDSGEQVLVDVETKSNKEIMEHIRKILGKNEETLREEEEEKKQLSHPANFGPRKYCLRECI
CEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_071942
RefSeq Size 4574
RefSeq ORF 519
Synonyms COXPD50; MRP-S25; RPMS25
Locus ID 64432
UniProt ID P82663
Cytogenetics 3p25.1
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2016
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Other "MRPS25" proteins (3)
SKU Description Size Price
LC411645 Lyophilized MRPS25 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY411645 Transient overexpression lysate of mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein (as WB positive control) 100 ug
$436.00
TP301353 Recombinant protein of human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein, 20 µg 20 ug
$867.00

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