APPD (PLEKHF1) (NM_024310) Human Mass Spec Standard

SKU
PH301282
PLEKHF1 MS Standard C13 and N15-labeled recombinant protein (NP_077286)
  $3,360.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC201282
Predicted MW 31.2 kDa
Protein Sequence
Protein Sequence
>RC201282 protein sequence
Red=Cloning site Green=Tags(s)

MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLN
KRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRP
PSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRE
LAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_077286
RefSeq Size 1774
RefSeq ORF 837
Synonyms APPD; LAPF; PHAFIN1; ZFYVE15
Locus ID 79156
UniProt ID Q96S99
Cytogenetics 19q12
Summary May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.UniProtKB/Swiss-Prot Function
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Other "APPD" proteins (3)
SKU Description Size Price
LC402983 Lyophilized PLEKHF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402983 Transient overexpression lysate of pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1) (as WB positive control) 100 ug
$436.00
TP301282 Recombinant protein of human pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), 20 µg 20 ug
$867.00

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