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PRKX (NM_005044) Human Untagged Clone

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SKU
SC323612
PRKX (untagged)-Kinase deficient mutant (K78M) of Human protein kinase, X-linked (PRKX)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PRKX
Synonyms PKX1
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC323612 sequence for NM_005044 edited (data generated by NextGen Sequencing)
ATGGAGGCGCCCGGGCTGGCCCAGGCGGCCGCGGCGGAGAGCGACTCCCGCAAGGTGGCG
GAGGAGACCCCCGACGGGGCGCCCGCGCTCTGCCCCAGCCCTGAGGCGCTGTCGCCGGAG
CCGCCTGTGTACAGCCTGCAGGACTTTGACACGCTGGCCACCGTGGGCACTGGGACTTTC
GGGCGGGTGCACCTGGTGAAGGAGAAGACAGCCAAGCATTTCTTCGCCCTCATGGTGATG
AGCATTCCCGACGTCATCCGCCTAAAGCAGGAGCAACACGTACACAATGAGAAGTCTGTC
CTGAAGGAAGTCAGCCACCCGTTCCTCATCAGGCTGTTCTGGACGTGGCATGACGAGCGC
TTCCTCTACATGCTCATGGAGTACGTGCCGGGCGGCGAGCTCTTCAGCTACCTGCGCAAC
CGGGGGCGCTTCTCCAGCACCACGGGGCTCTTCTACTCTGCAGAGATCATCTGTGCCATC
GAGTACCTGCACTCCAAAGAGATCGTCTACAGGGACTTGAAGCCAGAGAACATCCTGCTG
GATAGGGATGGCCACATTAAGCTCACGGACTTTGGGTTCGCCAAGAAGCTGGTAGACAGG
ACTTGGACCCTCTGTGGAACACCCGAGTACCTAGCCCCCGAAGTCATTCAGAGCAAGGGC
CACGGAAGGGCCGTGGACTGGTGGGCCCTCGGCATCCTGATATTCGAGATGCTTTCGGGG
TTTCCTCCGTTTTTTGATGACAACCCGTTTGGCATTTATCAGAAAATTCTTGCAGGCAAA
ATAGATTTCCCCAGACATTTGGATTTCCATGTAAAAGACCTCATTAAGAAACTGCTCGTG
GTTGACAGAACAAGGCGATTAGGAAACATGAAGAACGGGGCGAATGATGTGAAGCATCAT
CGGTGGTTCCGCTCCGTGGACTGGGAAGCTGTTCCGCAGAGAAAACTGAAGCCTCCCATC
GTGCCCAAGATAGCTGGTGACGGCGACACTTCCAACTTCGAAACTTACCCTGAGAATGAC
TGGGACACAGCCGCGCCCGTGCCGCAGAAGGATTTAGAAATCTTCAAGAATTTCTGA

Clone variation with respect to NM_005044.4
177 g=>t;233 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_005044 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCAACAGCGCGCA
CGCGGCTACCGAGCTGGAGGAGGCGGCGGGCGCGAGACCCGGAATGCGCAGGGCCCCCGCCTCGCCCCCC
CCAGCCCGGGCCGCGGCCCCCGCCTTCCCCGCAGTCGTCCCGCACTCGGTGCCCGCCCCCCGAGGCCGGC
GGCTGCTCCCACTCGGGGCCGTTGCTGCTTTGTGCCGTGAGCGCCGCCCAGCCATTGTCCCCGTCGCTTC
CGTCAGCCCGCGCCGGAACCGCGCACCAGGAAGGCCAAGAACCGCCCCATGGGGGAGGCCCTCGGTGGAT
GCGGCGGCGGGGCGGCCTTCGAGAGGCTGCTTCCGGGGAGGCCGGGTTCCCCAAGCCCTCGGGCTTGGCC
GGGGGCCCGGGACTGGGATGGCGTCCCCAGGGGGGGCCCCGGGCTGGCCCAGGGGGGCCGGGGGGGGAGA
CCAACCCCCGCAGGGGGGGGGAAGGAAACCCCAAAGGGGGCCCCCGCCCTTGTGCCCAACCCGAGGGGCC
TTTCCCGGGACCCCCCTGGGCCCCCCGGAAAATTTGACCCCGGGGCCCGTGGGGCATTGGACTTTTGGGG
GGGGTCCCGCGTAGGAAAAAACCCCCGTTTTTCCCCCCTTAGGGAGAAAAAAGAAG
Kinase Domain Sequence
>SC323612 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TMKKGCMCGTGGCMTGGGACTTTCGGGCGGGTGCACCTGGTGAAGGAGAAGACAGCCAAGCATTTCTTCG
CCCTCATGGTGATGAGCATTCCCGACGTCATCCGCCTAAAGCAGGAGCAACACGTACACAATGAGAAGTC
TGTCCTGAAGGAAGTCAGCCACCCGTTCCTCATCAGGCTGTTCTGGACGTGGCATGACGAGCGCTTCCTC
TACATGCTCATGGAGTACGTGCCGGGCGGCGAGCTCTTCAGCTAC
Restriction Sites Please inquire |
ACCN NM_005044
Insert Size 2710 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005044.1, NP_005035.1
RefSeq Size 6034 bp
RefSeq ORF 1077 bp
Locus ID 5613
UniProt ID P51817
Cytogenetics Xp22.33
Domains pkinase, S_TKc, S_TK_X, TyrKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
Summary This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y. provided by RefSeq, Feb 2010
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Documents
Resources
"NM_005044" in other vectors (7)
SKU Description Size Price
RC208332 PRKX (Myc-DDK-tagged)-Human protein kinase, X-linked (PRKX) 10 ug
$457.00
RC208332L1 Lenti ORF clone of Human protein kinase, X-linked (PRKX), Myc-DDK-tagged 10 ug
$757.00
RC208332L2 Lenti ORF clone of Human protein kinase, X-linked (PRKX), mGFP tagged 10 ug
$757.00
RC208332L3 Lenti ORF clone of Human protein kinase, X-linked (PRKX), Myc-DDK-tagged 10 ug
$757.00
RC208332L4 Lenti ORF clone of Human protein kinase, X-linked (PRKX), mGFP tagged 10 ug
$757.00
RG208332 PRKX (tGFP-tagged) - Human protein kinase, X-linked (PRKX) 10 ug
$489.00 $657.00
SC116959 PRKX (untagged)-Human protein kinase, X-linked (PRKX) 10 ug
$457.00

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