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HADHB (NM_000183) Human Untagged Clone

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SKU
SC320121
HADHB (untagged)-Human hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit (HADHB), nuclear gene encoding mitochondrial protein
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HADHB
Synonyms ECHB; MSTP029; MTPB; TP-BETA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000183.2 CCCTTGGTCCCGCAGAGCCTTGGTACTTGGACCTGAACCTTGCTCCGAGAGGGAGTCCTC
GCGGACGTCAGCCAAGATTCCAGAATGACTACTATCTTGACTTACCCCTTTAAAAATCTT
CCCACTGCATCAAAATGGGCCCTCAGATTTTCCATAAGACCTCTGAGCTGTTCCTCCCAG
CTACGAGCTGCCCCAGCTGTCCAGACCAAAACGAAGAAGACGTTAGCCAAACCCAATATA
AGGAATGTTGTGGTGGTGGATGGTGTTCGCACTCCATTTTTGCTGTCTGGCACTTCATAT
AAAGACCTGATGCCACATGATTTGGCTAGAGCAGCGCTTACGGGTTTGTTGCATCGGACC
AGTGTCCCTAAGGAAGTAGTTGATTATATCATCTTTGGTACAGTTATTCAGGAAGTGAAA
ACAAGCAATGTGGCTAGAGAGGCTGCCCTTGGAGCTGGCTTCTCTGACAAGACTCCTGCT
CACACTGTCACCATGGCTTGTATCTCTGCCAACCAAGCCATGACCACAGGTGTTGGCTTG
ATTGCTTCTGGCCAGTGTGATGTGATCGTGGCAGGTGGTGTTGAGTTGATGTCCGATGTC
CCTATTCGTCACTCAAGGAAAATGAGAAAACTGATGCTTGATCTCAATAAGGCCAAATCT
ATGGGCCAGCGACTGTCTTTAATCTCTAAATTCCGATTTAATTTCCTAGCACCTGAGCTC
CCTGCGGTTTCTGAGTTCTCCACCAGTGAGACCATGGGCCACTCTGCAGACCGACTGGCC
GCTGCCTTTGCTGTTTCTCGGCTGGAACAGGATGAATATGCACTGCGCTCTCACAGTCTA
GCCAAGAAGGCACAGGATGAAGGACTCCTTTCTGATGTGGTACCCTTCAAAGTACCAGGA
AAAGATACAGTTACCAAAGATAATGGCATCCGTCCTTCCTCACTGGAGCAGATGGCCAAA
CTAAAACCTGCATTCATCAAGCCCTACGGCACAGTGACAGCTGCAAATTCTTCTTTCTTG
ACTGATGGTGCATCTGCAATGTTAATCATGGCGGAGGAAAAGGCTCTGGCCATGGGTTAT
AAGCCGAAGGCATATTTGAGGGATTTTATGTATGTGTCTCAGGATCCAAAAGATCAACTA
TTACTTGGACCAACATATGCTACTCCAAAAGTTCTAGAAAAGGCAGGATTGACCATGAAT
GATATTGATGCTTTTGAATTTCATGAAGCTTTCTCGGGTCAGATTTTGGCAAATTTTAAA
GCCATGGATTCTGATTGGTTTGCAGAAAACTACATGGGTAGAAAAACCAAGGTTGGATTG
CCTCCTTTGGAGAAGTTTAATAACTGGGGTGGATCTCTGTCCCTGGGACACCCATTTGGA
GCCACTGGCTGCAGGTTGGTCATGGCTGCTGCCAACAGATTACGGAAAGAAGGAGGCCAG
TATGGCTTAGTGGCTGCGTGTGCAGCTGGAGGGCAGGGCCATGCTATGATAGTGGAAGCT
TATCCAAAATAATAGATCCAGAAGAAGTGACCTGAAGTTTCTGTGCAACACTCACACTAG
GCAATGCCATTTCAATGCATTACTAAATGACATTTGTAGTTCCTAGCTCCTCTTAGGAAA
ACAGTTCTTGTGGCCTTCTATTAAATACTTTGCACTTAAGCCTTGCCAGTGTTCTGAGCT
TTTCAATAATCAGTTTACTGCTCTTTCAGGGATTTCTAAGCCACCAGAATCTCACATGAG
ATGTGTGGGTGGTTGTTTTTGGTCTCTGTTGTCACTAAAGACTAAATGAGGGTTTGCAGT
TGGGAAAGAGGTCAACTGAGATTTGGAAATCATCTTTGTAATATTTGCAAATTATACTTG
TTCTTATCTGTGTCCTAAAGATGTGTTCTCTATAAAATACAAACCAACGTGCCTAATTAA
TTATGGAAAAATAATTCAGAATCTAAACACCACTGAAAACTTATAAAAAATGTTTAGATA
CATAAATATGGTGGTCAGCGTTAATAAAGTGGAGAAATATTGGAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_000183
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000183.2, NP_000174.1
RefSeq Size 2196 bp
RefSeq ORF 1425 bp
Locus ID 3032
UniProt ID P55084
Cytogenetics 2p23.3
Domains thiolase
Protein Pathways Fatty acid elongation in mitochondria, Fatty acid metabolism, leucine and isoleucine degradation, Metabolic pathways, Valine
Summary This gene encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Mutations in this gene result in trifunctional protein deficiency. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jul 2013
Transcript Variant: This variant (1) encodes the longest protein (isoform 1).
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Documents
Resources
"NM_000183" in other vectors (4)
SKU Description Size Price
RC209853 HADHB (Myc-DDK-tagged)-Human hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit (HADHB), nuclear gene encoding mitochondrial pr 10 ug
$457.00
RC209853L3 Lenti ORF clone of Human hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit (HADHB), nuclear gene encoding mitochondrial pr, Myc-DDK-tagged 10 ug
$757.00
RC209853L4 Lenti ORF clone of Human hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit (HADHB), nuclear gene encoding mitochondrial pr, mGFP tagged 10 ug
$757.00
RG209853 HADHB (tGFP-tagged) - Human hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit (HADHB), nuclear gene encoding mitochondrial protei 10 ug
$489.00 $657.00

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