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MAST3 (NM_015016) Human Untagged Clone

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SKU
SC316595
MAST3 (untagged)-Human microtubule associated serine/threonine kinase 3 (MAST3)
  $1,710.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAST3
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_015016 edited
ATGGACGAGTCGAGCCTCCTGCGGCGCCGCGGGCTCCAGAAGGAGCTGAGCCTGCCACGC
CGAGGACGTGGCTGCCGCAGCGGGAACCGCAAGAGCTTGGTGGTAGGAACGCCCTCCCCG
ACCCTCTCCCGGCCCCTGTCGCCATTGTCGGTCCCAACGGCAGGCAGCAGCCCCTTGGAT
AGTCCTCGGAATTTCTCGGCTGCCTCTGCCCTAAATTTCCCCTTTGCCCGGAGGGCAGAC
GGCAGAAGATGGTCCCTCGCGTCTCTCCCATCTTCCGGCTATGGAACCAACACACCCAGC
TCCACCCTCTCGTCAAGCTCATCCTCCCGGGAACGTCTCCACCAGCTTCCCTTCCAGCCG
ACGCCGGACGAGCTGCACTTCCTGTCCAAGCACTTCCGCAGCTCAGAGAATGTGCTTGAT
GAGGAAGGCGGCCGGTCACCCCGCCTCCGACCCCGCTCTCGCAGTCTCAGCCCGGGCCGT
GCAACGGGGACCTTCGACAATGAGATTGTCATGATGAATCACGTGTACCGGGAGAGGTTC
CCCAAGGCCACAGCACAGATGGAGGGCCGTCTGCAGGAGTTCCTGACGGCCTACGCGCCC
GGCGCCCGGCTGGCGCTGGCTGATGGCGTCTTGGGCTTCATCCACCACCAGATCGTCGAG
CTGGCCCGAGACTGCTTGGCCAAGTCTGGCGAGAACCTCGTCACCTCCCGCTACTTCCTA
GAGATGCAGGAGAAGCTGGAGCGGCTTCTGCAGGATGCCCATGAGCGTTCGGACAGTGAG
GAGGTCAGCTTCATCGTCCAGCTTGTCCGGAAACTGCTGATCATCATCTCACGGCCAGCT
CGGCTGCTGGAGTGTCTGGAGTTTGACCCTGAGGAATTTTACCACCTGCTGGAGGCGGCT
GAGGGCCATGCGCGGGAGGGCCAAGGCATTAAGACTGACCTTCCACAGTACATCATTGGG
CAGCTGGGCCTGGCCAAGGACCCCCTGGAGGAGATGGTGCCACTGAGTCACCTCGAAGAA
GAACAGCCCCCAGCACCTGAGTCCCCAGAGAGCCGCGCCCTGGTCGGCCAGTCACGGAGG
AAGCCATGCGAAAGCGACTTTGAGACCATCAAACTCATTAGCAACGGAGCCTATGGGGCC
GTCTACCTGGTGCGGCACCGTGACACACGGCAGCGCTTTGCCATCAAGAAGATCAACAAA
CAGAACTTGATCCTGCGTAACCAGATCCAGCAGGTCTTTGTGGAGCGTGACATTCTCACC
TTTGCCGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCCGGCGCCACCTA
TGTATGGTCATGGAATACGTGGAAGGCGGCGACTGCGCCACGCTCCTGAAGAACATGGGC
CCGCTGCCCGTGGACATGGCCCGCCTGTACTTCGCCGAGACGGTGTTGGCGCTGGAGTAC
CTGCATAACTATGGCATCGTGCACCGTGACCTCAAACCAGACAATCTGCTCATCACCTCG
CTTGGCCACATCAAGCTCACGGACTTCGGCCTGTCCAAGATCGGCCTCATGAGCATGGCC
ACCAACCTCTATGAGGGCCACATCGAGAAGGACGCCCGAGAGTTCATCGACAAGCAGGTG
TGTGGGACGCCGGAGTACATAGCCCCCGAGGTGATCTTCCGCCAGGGCTATGGGAAGCCA
GTGGACTGGTGGGCCATGGGCGTCGTCCTCTATGAGTTTCTGGTGGGCTGCGTGCCTTTC
TTTGGAGATACCCCCGAGGAACTCTTCGGTCAGGTGGTCAGCGATGAGATCATGTGGCCA
GAGGGAGATGAGGCCCTTCCAGCAGACGCCCAGGACCTCATCACCAGGTTGCTCCGGCAG
AGCCCGCTGGACCGTCTGGGCACTGGTGGCACCCACGAAGTGAAGCAGCACCCCTTTTTC
CTGGCCCTGGACTGGGCAGGGCTTCTCCGACACAAAGCCGAGTTCGTGCCCCAGCTCGAA
GCCGAGGATGATACCAGCTACTTTGACACACGTTCGGAACGTTACCGCCATCTGGGCTCC
GAGGACGACGAGACCAATGATGAAGAATCGTCCACAGAGATCCCCCAGTTCTCCTCCTGC
TCCCACCGGTTCAGCAAGGTCTACAGCAGCTCTGAGTTCCTGGCCGTCCAGCCCACTCCT
ACCTTCGCTGAAAGGAGCTTCAGTGAAGACCGGGAGGAGGGGTGGGAGCGCAGCGAAGTG
GACTATGGCCGCCGGCTGAGTGCTGACATCCGGCTGAGGTCCTGGACATCCTCTGGATCC
TCCTGTCAGTCATCTTCGTCCCAGCCCGAGCGGGGTCCCAGCCCATCTCTCCTGAATACC
ATCAGCCTGGACACAATGCCCAAGTTTGCCTTCTCATCAGAGGATGAGGGGGTAGGCCCA
GGCCCTGCAGGCCCCAAGAGGCCCGTCTTCATTCTAGGGGAGCCTGACCCCCCACCAGCG
GCCACCCCAGTGATGCCCAAGCCCTCGAGCCTTTCTGCCGACACAGCTGCTCTCAGCCAC
GCCCGCCTACGGAGCAATAGCATCGGCGCCCGACACTCCACACCAAGGCCTCTGGATGCC
GGCCGGGGCCGCCGCCTTGGGGGCCCAAGAGACCCAGCCCCTGAGAAGTCCAGAGCCTCC
TCCAGCGGTGGCAGTGGTGGCGGCAGTGGGGGCCGCGTGCCCAAGTCAGCCTCTGTCTCT
GCCCTGTCCCTCATCATCACGGCAGATGATGGCAGCGGCGGCCCCCTCATGAGCCCCCTT
TCCCCGCGCTCTCTGTCCTCGAACCCGTCGTCCCGTGACTCTTCGCCGAGCCGAGACCCG
TCCCCCGTGTGTGGCAGCCTGCGGCCCCCCATCGTTATCCACAGCTCTGGCAAGAAGTAC
GGCTTCAGCCTGCGGGCGATCCGCGTCTACATGGGTGATAGCGACGTCTACACTGTGCAC
CACGTCGTCTGGAGTGTGGAGGACGGAAGCCCCGCCCAGGAGGCGGGCCTGCGGGCTGGG
GACCTCATCACCCACATCAACGGGGAGTCAGTGCTGGGGCTGGTGCACATGGACGTCGTG
GAGCTGCTGCTGAAGAGCGGCAACAAGATATCCCTGCGGACCACAGCCCTGGAGAACACC
TCCATCAAGGTGGGCCCCGCCCGGAAGAATGTGGCCAAGGGCCGCATGGCACGCAGGAGC
AAGAGGAGCCGTCGGCGGGAGACCCAGGATCGGCGGAAGTCACTTTTCAAGAAGATCTCC
AAGCAGACCTCCGTGCTGCACACCAGCCGCAGCTTCTCCTCCGGACTCCACCACTCACTG
TCATCCAGTGAGAGCCTCCCCGGCTCGCCCACCCACAGCCTCTCCCCCAGCCCCACCACT
CCCTGCCGAAGCCCAGCCCCTGATGTCCCAGCAGATACCACTGCATCCCCACCCAGCGCA
TCCCCGAGCTCCAGCAGCCCCGCCTCCCCAGCTGCTGCTGGCCACACCCGCCCCAGCTCC
CTGCACGGCCTGGCTGCCAAGCTTGGGCCACCCCGCCCCAAGACTGGCCGCCGCAAGTCC
ACCAGCAGCATCCCGCCCTCCCCGCTGGCCTGCCCGCCCATCTCCGCGCCCCCACCCCGC
TCGCCCTCGCCCCTGCCCGGGCACCCGCCCGCACCTGCCCGATCCCCGCGGCTGCGCCGG
GGCCAGTCAGCTGACAAGCTGGGCACAGGGGAGCGGCTGGATGGGGAGGCGGGGCGGCGC
ACTCGTGGGCCAGAGGCCGAGCTCGTGGTCATGCGGCGGCTGCACCTGTCCGAGCGCCGA
GACTCCTTCAAGAAGCAGGAGGCCGTGCAGGAGGTTAGCTTCGATGAGCCGCAGGAGGAG
GCCACTGGGCTGCCCACCTCAGTGCCACAGATCGCCGTGGAGGGCGAGGAAGCCGTGCCA
GTAGCTCTCGGGCCCACCGGAAGAGACTGA
Restriction Sites Please inquire |
ACCN NM_015016
Insert Size 6000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation It is not a varient. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015016.1, NP_055831.1
RefSeq Size 5893 bp
RefSeq ORF 3930 bp
Locus ID 23031
UniProt ID O60307
Cytogenetics 19p13.11
Protein Families Druggable Genome, Protein Kinase
Reviews
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Documents
Resources
"NM_015016" in other vectors (7)
SKU Description Size Price
RC219098 MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) 10 ug
$1,709.00
RC219098L1 Lenti-ORF clone of MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) 10 ug
$2,009.00
RC219098L2 Lenti-ORF clone of MAST3 (mGFP-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) 10 ug
$2,009.00
RC219098L3 Lenti-ORF clone of MAST3 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) 10 ug
$2,009.00
RC219098L4 Lenti-ORF clone of MAST3 (mGFP-tagged)-Human microtubule associated serine/threonine kinase 3 (MAST3) 10 ug
$2,009.00
RG219098 MAST3 (tGFP-tagged) - Human microtubule associated serine/threonine kinase 3 (MAST3) 10 ug
$1,909.00
SC323438 MAST3 (untagged)-Kinase deficient mutant (K226M) of Human microtubule associated serine/threonine kinase 3 (MAST3) 10 ug
$1,710.00

file-alt Citations

Genome-wide expression profiling implicates a MAST3-regulated gene set in colonic mucosal inflammation of ulcerative colitis patients
Labbé, Boucher, Foisy et al
Inflamm. Bowel Dis. (2012) 18 (6), 1072-80 DOI: 10.1002/ibd.21887
Cited by 31
AI Summary: In this study, OriGene's pCMV-XL6 vector containing MAST3 cDNA (SC316595) and pCMV-AC-GFP vector (PS100010) were used to modulate MAST3 expression in cell lines, enabling the authors to investigate the gene's role in regulating inflammatory responses relevant to ulcerative colitis.
Snippet: At day 1, cells were transiently transfected using 6 μg of pCMV-XL6 vector containing MAST3 cDNA (SC316595;Origene, Rockville, MD) or 6 μg of pCMV-AC-GFP vector (PS100010;Origene) and 15 μL of lipofectamin 2000 (Invitrogen, Carlsbad, CA) according to the
Showing 1-1 of 1 papers.
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