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GIT1 (NM_001085454) Human Untagged Clone

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SKU
SC316259
GIT1 (untagged)-Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 1
  $777.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GIT1
Vector pCMV6 series
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_001085454, the custom clone sequence may differ by one or more nucleotides
ATGTCCCGAAAGGGGCCGCGAGCGGAGGTGTGTGCGGACTGCAGCGCCCCGGACCCTGGC
TGGGCATCCATCAGCAGGGGTGTGCTGGTGTGTGACGAGTGCTGCAGCGTGCACCGGAGC
CTGGGACGCCACATCTCCATTGTCAAGCACCTTCGCCACAGCGCCTGGCCTCCCACGCTG
CTGCAGATGGTGCACACGCTTGCCAGCAACGGGGCCAACTCCATCTGGGAGCACTCCCTG
CTGGACCCCGCACAAGTGCAGAGCGGCCGGCGTAAAGCCAACCCCCAAGACAAAGTCCAC
CCCATCAAGTCAGAGTTCATCAGGGCCAAGTACCAGATGCTGGCATTTGTGCACAAGCTT
CCCTGCCGGGACGATGATGGAGTCACCGCCAAAGACCTCAGCAAGCAACTACACTCGAGC
GTGCGGACAGGCAACCTGGAGACATGTCTGCGCCTGCTCTCCCTGGGTGCCCAGGCCAAC
TTCTTCCACCCAGAGAAGGGCACCACACCTCTGCACGTGGCTGCCAAGGCAGGACAGACA
CTGCAGGCCGAGCTGCTTGTAGTGTATGGGGCTGACCCTGGCTCCCCTGATGTTAATGGC
CGCACACCCATTGACTATGCCAGGCAGGCGGGGCACCATGAGCTGGCGGAAAGGCTGGTT
GAGTGCCAATATGAGCTCACTGACCGGCTGGCCTTCTACCTCTGTGGACGCAAGCCGGAT
CACAAGAATGGGCATTACATCATCCCACAGATGGCTGACAGATCTCGGCAAAAGTGCATG
TCTCAGAGCCTTGACTTATCCGAATTGGCCAAAGCTGCTAAGAAGAAGCTGCAGGCGCTC
AGCAACCGGCTTTTTGAGGAACTCGCCATGGACGTGTATGACGAGGTGGATCGAAGAGAA
AATGATGCAGTGTGGCTGGCTACCCAAAACCACAGCACTCTGGTGACAGAGCGCAGTGCC
GTGCCCTTCCTGCCTGTTAACCCGGAATACTCAGCCACGCGGAATCAGGGGCGACAAAAG
CTGGCCCGCTTTAATGCCCGAGAGTTTGCCACCTTGATCATCGACATTCTCAGTGAGGCC
AAGCGGAGACAGCAGGGCAAGAGCCTGAGCAGCCCCACAGACAACCTCGAGCTGTCTCTG
CGGAGCCAGAGTGACCTCGACGACCAACACGACTACGACAGCGTGGCCTCTGACGAGGAC
ACAGACCAGGAGCCCCTGCGCAGCACCGGCGCCACTCGGAGCAACCGGGCCCGGAGCATG
GACTCCTCGGACTTGTCTGACGGGGCTGTGACGCTGCAGGAGTACCTGGAGCTGAAGAAG
GCCCTGGCTACATCGGAGGCAAAGGTGCAGCAGCTCATGAAGGTCAACAGTAGCCTGAGC
GACGAGCTCCGGAGGCTGCAGCGAGAGATCCACAAGCTGCAGGCGGAGAACCTGCAGCTC
CGGCAGCCTCCAGGGCCGGTGCCCACACCTCCACTCCCCAGTGAACGGGCGGAACACACA
CCCATGGCGCCAGGCGGGAGCACACACCGCAGGGATCGCCAGGCCTTTTCCATGTATGAA
CCTGGCTCTGCCCTGAAGCCCTTTGGGGGCCCCCCTGGGGACGAGCTCACTACGCGGCTG
CAGCCTTTCCACAGCACTGAGCTAGAGGACGACGCCATCTATTCAGTGCACGTCCCTGCT
GGCCTTTACCGGATCCGGAAAGGGGTGTCTGCCTCAGCTGTGCCCTTCACTCCCTCCTCC
CCGCTGCTGTCCTGCTCCCAGGAGGGAAGCCGCCACACGAGCAAGCTTTCCCGCCACGGC
AGTGGAGCCGACAGTGACTATGAGAACACGCAAAGTGGGGACCCACTGCTGGGGCTGGAA
GGGAAGAGGTTTCTAGAGCTGGGCAAAGAGGAAGACTTCCACCCAGAGCTGGAAAGCCTG
GATGGAGACCTAGATCCTGGGCTTCCCAGCACAGAGGATGTCATCTTGAAGACAGAGCAG
GTCACCAAGAACATTCAGGAACTGTTGCGGGCAGCCCAGGAGTTCAAGCATGACAGCTTC
GTGCCCTGCTCAGAGAAGATCCATTTGGCTGTGACCGAGATGGCCTCCCTCTTCCCAAAG
AGGCCAGCCCTGGAGCCAGTGCGGAGCTCACTGCGGCTGCTCAACGCCAGCGCCTACCGG
CTGCAGAGTGAGTGCCGGAAGACAGTGCCCCCAGAGCCCGGCGCCCCAGTGGACTTCCAG
CTGCTGACTCAGCAGGTGATCCAGTGCGCCTATGACATCGCCAAGGCTGCCAAGCAGCTG
GTCACCATCACCACCCGAGAGAAGAAGCAG
Restriction Sites Please inquire |
ACCN NM_001085454
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001085454.1, NP_001078923.1
RefSeq Size 3778 bp
RefSeq ORF 2313 bp
Locus ID 28964
UniProt ID Q9Y2X7
Cytogenetics 17q11.2
Protein Families Druggable Genome
Protein Pathways Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton
Summary GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_001085454" in other vectors (4)
SKU Description Size Price
RC224712 GIT1 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 1 10 ug
$589.00 $705.00
RC224712L3 Lenti-ORF clone of GIT1 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 1 10 ug
$1,005.00
RC224712L4 Lenti-ORF clone of GIT1 (mGFP-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 1 10 ug
$1,005.00
RG224712 GIT1 (tGFP-tagged) - Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 1 10 ug
$789.00 $905.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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