CCN1 (CYR61) (NM_001554) Human Untagged Clone

SKU SC310465

CYR61 (untagged)-Human cysteine-rich, angiogenic inducer, 61 (CYR61)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CCN1
• Synonyms: CYR61; GIG1; IGFBP10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC310465 sequence for NM_001554 edited (data generated by NextGen Sequencing); ATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGTCACCCTTCTCCACTTGACCAGG; CTGGCGCTCTCCACCTGCCCCGCTGCCTGCCACTGCCCCCTGGAGGCGCCCAAGTGCGCG; CCGGGAGTCGGGCTGGTCCGGGACGGCTGCGGCTGCTGTAAGGTCTGCGCCAAGCAGCTC; AACGAGGACTGCAGCAAAACGCAGCCCTGCGACCACACCAAGGGGCTGGAATGCAACTTC; GGCGCCAGCTCCACCGCTCTGAAGGGGATCTGCAGAGCTCAGTCAGAGGGCAGACCCTGT; GAATATAACTCCAGAATCTACCAAAACGGGGAAAGTTTCCAGCCCAACTGTAAACATCAG; TGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCTGTGTCCCCAAGAACTATCTCTC; CCCAACTTGGGCTGTCCCAACCCTCGGCTGGTCAAAGTTACCGGGCAGTGCTGCGAGGAG; TGGGTCTGTGACGAGGATAGTATCAAGGACCCCATGGAGGACCAGGACGGCCTCCTTGGC; AAGGAGCTGGGATTCGATGCCTCCGAGGTGGAGTTGACGAGAAACAATGAATTGATTGCA; GTTGGAAAAGGCAGCTCACTGAAGCGGCTCCCTGTTTTTGGAATGGAGCCTCGCATCCTA; TACAACCCTTTACAAGGCCAGAAATGTATTGTTCAAACAACTTCATGGTCCCAGTGCTCA; AAGACCTGTGGAACTGGTATCTCCACACGAGTTACCAATGACAACCCTGAGTGCCGCCTT; GTGAAAGAAACCCGGATTTGTGAGGTGCGGCCTTGTGGACAGCCAGTGTACAGCAGCCTG; AAAAAGGGCAAGAAATGCAGCAAGACCAAGAAATCCCCCGAACCAGTCAGGTTTACTTAC; GCTGGATGTTTGAGTGTGAAGAAATACCGGCCCAAGTACTGCGGTTCCTGCGTGGACGGC; CGATGCTGCACGCCCCAGCTGACCAGGACTGTGAAGATGCGGTTCCGCTGCGAAGATGGG; GAGACATTTTCCAAGAACGTCATGATGATCCAGTCCTGCAAATGCAACTACAACTGCCCG; CATGCCAATGAAGCAGCGTTTCCCTTCTACAGGCTGTTCAATGACATTCACAAATTTAGG; GACTAA; ; Clone variation with respect to NM_001554.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001554 unedited; NGGCGGTCAAAATTGTATACGACTCCTATAGGCGGCCCGCGAATCGGCACCAGGCGCCCC; CGAGCAGCGCCCGCGCCCTCCGCGCCTTCTCCGCCGGGACCTCGAGCGAAAGACGCCCGC; CCGCCGCCCAGCCCTCGCCTCCCTGCCCACCGGGCACACCGCGCCGCCACCCCGACCCCG; CTGCGCACGGCCTGTCCGCTGCACACCAGCTTGTTGGCGTCTTCGTCGCCGCGCTCGCCC; CGGGCTACTCCTGCGCGCCACAATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGT; CACCCTTCTCCACTTGACCAGGCTGGCGCTCTCCACCTGCCCCGCTGCCTGCCACTGCCC; CCTGGAGGCGCCCAAGTGCGCGCCGGGAGTCGGGCTGGTCCGGGACGGCTGCGGCTGCTG; TAAGGTCTGCGCCAAGCAGCTCAACGAGGACTGCAGCAAAACGCAGCCCTGCGACCACAC; CAAGGGGCTGGAATGCAACTTCGGCGCCAGCTCCACCGCTCTGAAGGGGATCTGCAGAGC; TCAGTCAGAGGGCAGACCCTGTGAATATAACTCCAGAATCTACCAAAACGGGGAAAGTTT; CCAGCCCAACTGTAAACATCAGTGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCT; GTGTCCCCAAGAACTATCTCTCCCCAACTTGGGCTGTCCCAACCCTCGGCTGGTCAAAGT; TACCGGGCAGTGCTGCGAGGAGTGGGTCTGTGACGAGGATAGTATCAAGGACCCCATGGN; AGACCAGGACNGCCTCCTTGGCAGGAGCTGGGATTCGATGCCTCCGAGGTGGAGTTGACG; AGAACAATGAATGATNGCAGTTGGAAAAGGCAGCTCACTGAAGCGGCTCCCTGGNTTTGG; AATGGAGCTCGCATCTATACACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001554 unedited; AGGNANAGCACTGGGGNAGGGTCACAGGCTGCCACCCGGGTATCTGTTGAGGCAAACAGC; TATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTAGGTAAAT; ATTTCTTTATTAAATGGTTAATTCCATAAATAAAATCTTTTCACTGAATATAAAATTAAA; ATCATTTACAAATTATTCCAGTATTACATTTCCCCTCCCTCCCCAAAAGCTACATTTTGA; TAAATAAAACATTCAGTCTTAAAACACCTGATTTCTGTTTGCAGTTTAGAGTGCAGATAG; CTGCCTCTCACAGACACTCATGGAGTGTCCCCCTTCAGGAAGGGATGGAATGCCCTCCCA; TTTACTTTTGTCAAAGGACAAATAAAAGCTTTAAACTGTCCAACTAATCTTATTATCTCT; TTTACGACTGTAGAACCCCAAAAGGAAAAAAGCCTAGAGAAAATATGCTTAGCATATAAT; TTACAAACAGAAAACAGAATGTCATCAACTCCACAAGCTCCAAAATGAAGCAGTAACATG; TGCTCCAATACTATGAAGCAAAGTATTCTCCAATCGTGGCTGCATTAGTGTCCATCCGCA; CCAGCACCCTTGGCAGTTTCGAAATACCTTAATGCTCCTCAAGAATGAGCAAGGCTTCCT; TTCTACAATGAGTCCCATCACCCACACCACCCCCGCCCATTTTCTCCATGATTCTGATTC; TGACACTCTNTCTCCTTGTTTGTCTAGGTGTGCCCTGGAAACCCAGTAGCATTTAGTCCC; TAAATTTGTGAATGTCATTGCACAGCCTGTAGAAGGGAAACGCTGCTTATTGGCATGCGG; GCATTTGTAGTTGCATTTGCAGGACTGGATCATCATGACGTTCTTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001554
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001554.3, NP_001545.2
• RefSeq Size: 2037 bp
• RefSeq ORF: 1146 bp
• Locus ID: 3491
• UniProt ID: O00622
• Cytogenetics: 1p22.3
• Domains: CT, Cys_knot, IB, tsp_1, VWC
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Summary: The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]