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CCN1 (CYR61) (NM_001554) Human Untagged Clone

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SKU
SC310465
CYR61 (untagged)-Human cysteine-rich, angiogenic inducer, 61 (CYR61)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CCN1
Synonyms CYR61; GIG1; IGFBP10
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC310465 sequence for NM_001554 edited (data generated by NextGen Sequencing)
ATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGTCACCCTTCTCCACTTGACCAGG
CTGGCGCTCTCCACCTGCCCCGCTGCCTGCCACTGCCCCCTGGAGGCGCCCAAGTGCGCG
CCGGGAGTCGGGCTGGTCCGGGACGGCTGCGGCTGCTGTAAGGTCTGCGCCAAGCAGCTC
AACGAGGACTGCAGCAAAACGCAGCCCTGCGACCACACCAAGGGGCTGGAATGCAACTTC
GGCGCCAGCTCCACCGCTCTGAAGGGGATCTGCAGAGCTCAGTCAGAGGGCAGACCCTGT
GAATATAACTCCAGAATCTACCAAAACGGGGAAAGTTTCCAGCCCAACTGTAAACATCAG
TGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCTGTGTCCCCAAGAACTATCTCTC
CCCAACTTGGGCTGTCCCAACCCTCGGCTGGTCAAAGTTACCGGGCAGTGCTGCGAGGAG
TGGGTCTGTGACGAGGATAGTATCAAGGACCCCATGGAGGACCAGGACGGCCTCCTTGGC
AAGGAGCTGGGATTCGATGCCTCCGAGGTGGAGTTGACGAGAAACAATGAATTGATTGCA
GTTGGAAAAGGCAGCTCACTGAAGCGGCTCCCTGTTTTTGGAATGGAGCCTCGCATCCTA
TACAACCCTTTACAAGGCCAGAAATGTATTGTTCAAACAACTTCATGGTCCCAGTGCTCA
AAGACCTGTGGAACTGGTATCTCCACACGAGTTACCAATGACAACCCTGAGTGCCGCCTT
GTGAAAGAAACCCGGATTTGTGAGGTGCGGCCTTGTGGACAGCCAGTGTACAGCAGCCTG
AAAAAGGGCAAGAAATGCAGCAAGACCAAGAAATCCCCCGAACCAGTCAGGTTTACTTAC
GCTGGATGTTTGAGTGTGAAGAAATACCGGCCCAAGTACTGCGGTTCCTGCGTGGACGGC
CGATGCTGCACGCCCCAGCTGACCAGGACTGTGAAGATGCGGTTCCGCTGCGAAGATGGG
GAGACATTTTCCAAGAACGTCATGATGATCCAGTCCTGCAAATGCAACTACAACTGCCCG
CATGCCAATGAAGCAGCGTTTCCCTTCTACAGGCTGTTCAATGACATTCACAAATTTAGG
GACTAA

Clone variation with respect to NM_001554.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001554 unedited
NGGCGGTCAAAATTGTATACGACTCCTATAGGCGGCCCGCGAATCGGCACCAGGCGCCCC
CGAGCAGCGCCCGCGCCCTCCGCGCCTTCTCCGCCGGGACCTCGAGCGAAAGACGCCCGC
CCGCCGCCCAGCCCTCGCCTCCCTGCCCACCGGGCACACCGCGCCGCCACCCCGACCCCG
CTGCGCACGGCCTGTCCGCTGCACACCAGCTTGTTGGCGTCTTCGTCGCCGCGCTCGCCC
CGGGCTACTCCTGCGCGCCACAATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGT
CACCCTTCTCCACTTGACCAGGCTGGCGCTCTCCACCTGCCCCGCTGCCTGCCACTGCCC
CCTGGAGGCGCCCAAGTGCGCGCCGGGAGTCGGGCTGGTCCGGGACGGCTGCGGCTGCTG
TAAGGTCTGCGCCAAGCAGCTCAACGAGGACTGCAGCAAAACGCAGCCCTGCGACCACAC
CAAGGGGCTGGAATGCAACTTCGGCGCCAGCTCCACCGCTCTGAAGGGGATCTGCAGAGC
TCAGTCAGAGGGCAGACCCTGTGAATATAACTCCAGAATCTACCAAAACGGGGAAAGTTT
CCAGCCCAACTGTAAACATCAGTGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCT
GTGTCCCCAAGAACTATCTCTCCCCAACTTGGGCTGTCCCAACCCTCGGCTGGTCAAAGT
TACCGGGCAGTGCTGCGAGGAGTGGGTCTGTGACGAGGATAGTATCAAGGACCCCATGGN
AGACCAGGACNGCCTCCTTGGCAGGAGCTGGGATTCGATGCCTCCGAGGTGGAGTTGACG
AGAACAATGAATGATNGCAGTTGGAAAAGGCAGCTCACTGAAGCGGCTCCCTGGNTTTGG
AATGGAGCTCGCATCTATACACC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001554 unedited
AGGNANAGCACTGGGGNAGGGTCACAGGCTGCCACCCGGGTATCTGTTGAGGCAAACAGC
TATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTAGGTAAAT
ATTTCTTTATTAAATGGTTAATTCCATAAATAAAATCTTTTCACTGAATATAAAATTAAA
ATCATTTACAAATTATTCCAGTATTACATTTCCCCTCCCTCCCCAAAAGCTACATTTTGA
TAAATAAAACATTCAGTCTTAAAACACCTGATTTCTGTTTGCAGTTTAGAGTGCAGATAG
CTGCCTCTCACAGACACTCATGGAGTGTCCCCCTTCAGGAAGGGATGGAATGCCCTCCCA
TTTACTTTTGTCAAAGGACAAATAAAAGCTTTAAACTGTCCAACTAATCTTATTATCTCT
TTTACGACTGTAGAACCCCAAAAGGAAAAAAGCCTAGAGAAAATATGCTTAGCATATAAT
TTACAAACAGAAAACAGAATGTCATCAACTCCACAAGCTCCAAAATGAAGCAGTAACATG
TGCTCCAATACTATGAAGCAAAGTATTCTCCAATCGTGGCTGCATTAGTGTCCATCCGCA
CCAGCACCCTTGGCAGTTTCGAAATACCTTAATGCTCCTCAAGAATGAGCAAGGCTTCCT
TTCTACAATGAGTCCCATCACCCACACCACCCCCGCCCATTTTCTCCATGATTCTGATTC
TGACACTCTNTCTCCTTGTTTGTCTAGGTGTGCCCTGGAAACCCAGTAGCATTTAGTCCC
TAAATTTGTGAATGTCATTGCACAGCCTGTAGAAGGGAAACGCTGCTTATTGGCATGCGG
GCATTTGTAGTTGCATTTGCAGGACTGGATCATCATGACGTTCTTG
Restriction Sites NotI-NotI |
ACCN NM_001554
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001554.3, NP_001545.2
RefSeq Size 2037 bp
RefSeq ORF 1146 bp
Locus ID 3491
UniProt ID O00622
Cytogenetics 1p22.3
Domains CT, Cys_knot, IB, tsp_1, VWC
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Summary The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. provided by RefSeq, Sep 2011
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Documents
Resources
"NM_001554" in other vectors (6)
SKU Description Size Price
RC203492 CYR61 (Myc-DDK-tagged)-Human cysteine-rich, angiogenic inducer, 61 (CYR61) 10 ug
$686.00
RC203492L1 Lenti ORF clone of Human cysteine-rich, angiogenic inducer, 61 (CYR61), Myc-DDK-tagged 10 ug
$986.00
RC203492L2 Lenti ORF clone of Human cysteine-rich, angiogenic inducer, 61 (CYR61), mGFP tagged 10 ug
$986.00
RC203492L3 Lenti ORF clone of Human cysteine-rich, angiogenic inducer, 61 (CYR61), Myc-DDK-tagged 10 ug
$986.00
RC203492L4 Lenti ORF clone of Human cysteine-rich, angiogenic inducer, 61 (CYR61), mGFP tagged 10 ug
$986.00
RG203492 CYR61 (tGFP-tagged) - Human cysteine-rich, angiogenic inducer, 61 (CYR61) 10 ug
$489.00 $886.00

file-alt Citations

Identifying CYR61 as a Potential Anti-fibrotic and Pro-angiogenic Mediator in Scleroderma
Tsou, Khanna, Sawalha
Arthritis & rheumatology (Hoboken, N.J.) (2019)
AI Summary: OriGene’s CYR61 expression vector (pCMV6-XL4) was used to overexpress CYR61 in endothelial cells, enabling the authors to study its anti-fibrotic and pro-angiogenic roles in scleroderma.
Snippet: All rights reserved. CYR61 overexpression. Overexpression of CYR61 in ECs was performed as previously described (3). ECs were transfected with 1.65 μg of CYR61 (Origene;control vector pCMV6-XL4) and lipofectamine 2000 (3.3 μL, Invitrogen) for 24 hours in T12
Histone Deacetylase 5 Is Overexpressed in Scleroderma Endothelial Cells and Impairs Angiogenesis via Repression of Proangiogenic Factors
Tsou, Wren, Amin et al
Arthritis & rheumatology (2016) 68 (12), 2975-2985 DOI: 10.1002/art.39828
Cited by 72
AI Summary: OriGene products—specifically FSTL1 and PVRL2 (pCMV6-XL5 vectors) and CYR61 (pCMV6-XL4 vector)—were used to overexpress these genes in endothelial cells, enabling the authors to demonstrate their functional roles in regulating angiogenesis in scleroderma.
Snippet: Overexpression of PVRL2, FSTL1, and CYR61 was performed as previously described with some modification (4). ECs were transfected with 0.33 μg of FSTL1, PVRL2 (both from Origene;control vector pCMV6-XL5), or 1.65 μg of CYR61 (Origene;control vector pCMV6-XL4) and lipofectamine 2000 (Invitrogen) for 24 hours in T12.5 flasks
FigureFigure
Substance P-mediated expression of the pro-angiogenic factor CCN1 modulates the course of colitis
Koon, Zhao, Xu et al
Am J Pathol (2008) 173 (2), 400-10 DOI: 10.2353/ajpath.2008.080222
Cited by 40
AI Summary: OriGene products contributed to this study by providing the human CCN1 cDNA (Cat# RC204845) used for overexpression experiments and the CCN1 shRNA plasmid (Cat# TL313969) used for gene silencing, enabling the authors to investigate the role of CCN1 in substance P-mediated angiogenesis during colitis.
FigureFigureFigure
Showing 1-3 of 3 papers.
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