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PDE4D (NM_006203) Human Untagged Clone

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SKU
SC310179
PDE4D (untagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2
  $617.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PDE4D
Synonyms ACRDYS2; DPDE3; HSPDE4D; PDE4DN2; PDE43; STRK1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_006203 edited
ATGATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGAC
AATGGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGATCCGGGCTA
ATTCTCCAAGCAAATTTTGTCCACAGTCAACGACGGGAGTCCTTCCTGTATCGATCCGAC
AGCGATTATGACCTCTCTCCAAAGTCTATGTCCCGGAACTCCTCCATTGCCAGTGATATA
CACGGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCTTGGCCAGTCTGCGAACTGTA
CGAAACAACTTTGCTGCATTAACTAATTTGCAAGATCGAGCACCTAGCAAAAGATCACCC
ATGTGCAACCAACCATCCATCAACAAAGCCACCATAACAGAGGAGGCCTACCAGAAACTG
GCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTACAGACC
AGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTTAAAAGGATGCTTAATCGGGAGCTC
ACCCATCTCTCTGAAATGAGTCGGTCTGGAAATCAAGTGTCAGAGTTTATATCAAACACA
TTCTTAGATAAGCAACATGAAGTGGAAATTCCTTCTCCAACTCAGAAGGAAAAGGAGAAA
AAGAAAAGACCAATGTCTCAGATCAGTGGAGTCAAGAAATTGATGCACAGCTCTAGTCTG
ACTAATTCAAGTATCCCAAGGTTTGGAGTTAAAACTGAACAAGAAGATGTCCTTGCCAAG
GAACTAGAAGATGTGAACAAATGGGGTCTTCATGTTTTCAGAATAGCAGAGTTGTCTGGT
AACCGGCCCTTGACTGTTATCATGCACACCATTTTTCAGGAACGGGATTTATTAAAAACA
TTTAAAATTCCAGTAGATACTTTAATTACATATCTTATGACTCTCGAAGACCATTACCAT
GCTGATGTGGCCTATCACAACAATATCCATGCTGCAGATGTTGTCCAGTCTACTCATGTG
CTATTATCTACACCTGCTTTGGAGGCTGTGTTTACAGATTTGGAGATTCTTGCAGCAATT
TTTGCCAGTGCAATACATGATGTAGATCATCCTGGTGTGTCCAATCAATTTCTGATCAAT
ACAAACTCTGAACTTGCCTTGATGTACAATGATTCCTCAGTCTTAGAGAACCATCATTTG
GCTGTGGGCTTTAAATTGCTTCAGGAAGAAAACTGTGACATTTTCCAGAATTTGACCAAA
AAACAAAGACAATCTTTAAGGAAAATGGTCATTGACATCGTACTTGCAACAGATATGTCA
AAACACATGAATCTACTGGCTGATTTGAAGACTATGGTTGAAACTAAGAAAGTGACAAGC
TCTGGAGTTCTTCTTCTTGATAATTATTCCGATAGGATTCAGGTTCTTCAGAATATGGTG
CACTGTGCAGATCTGAGCAACCCAACAAAGCCTCTCCAGCTGTACCGCCAGTGGACGGAC
CGGATAATGGAGGAGTTCTTCCGCCAAGGAGACCGAGAGAGGGAACGTGGCATGGAGATA
AGCCCCATGTGTGACAAGCACAATGCTTCCGTGGAAAAATCACAGGTGGGCTTCATAGAC
TATATTGTTCATCCCCTCTGGGAGACATGGGCAGACCTCGTCCACCCTGACGCCCAGGAT
ATTTTGGACACTTTGGAGGACAATCGTGAATGGTACCAGAGCACAATCCCTCAGAGCCCC
TCTCCTGCACCTGATGACCCAGAGGAGGGCCGGCAGGGTCAAACTGAGAAATTCCAGTTT
GAACTAACTTTAGAGGAAGATGGTGAGTCAGACACGGAAAAGGACAGTGGCAGTCAAGTG
GAAGAAGACACTAGCTGCAGTGACTCCAAGACTCTTTGTACTCAAGACTCAGAGTCTACT
GAAATTCCCCTTGATGAACAGGTTGAAGAGGAGGCAGTAGGGGAAGAAGAGGAAAGCCAG
CCTGAAGCCTGTGTCATAGATGATCGTTCTCCTGACACG
5' Read Nucleotide Sequence
>Reverse primer walk for NM_006203 unedited
CTACATGTAAGGACTCCCGTCTGTGACTGTGGACAAAATTTGCTTGGACAATTAACCCGG
ATCCTGGGCTGCTCATGGGATCCAAGGGACTCCGTCCCGCAAATGTGCCATTGTCCACAT
CAAAACATATCCAGGAATGCCTTCTAGAGGGAAAATTATTCACGTGCATCATGTTCCCAT
ATCTTCTGTCATTAATATTTTTCTTGTCTCCTACGTTACATGTAGTGATTTTTACAGATG
AATTCCAACTCTCTTGTTTGAGCAACTATCGTGCGCAATTATTGCAACACGTATTGAATC
CCAAAAAAAAAAAAAAAATCTCCCCCCACCAATAAAAACAAATAGCACTTACAAAAGGCC
TTCCATGATTTGAAAAAACAAACATCACAGAACACTGCAGGTATGGATCCATCCATTTAG
GTTCCACTATTTAGTCTACATACTCAAATAACCAGTTCAACTAATAATCCTAACTGGCGG
CCAATATTGTCCTGGAAAAATCTAACCGCCTTGAGTGTGAATTAATGCATCCTTAACGAA
GGACTCTGGAATGTGGGAAGAGAGGAGTCAAAAGACAGTTTCTTGATATCCTTGCTAAAC
AAGTGTTCGCATGGAAATGGAAAACTTCAGGTTGCTTTGCCATGCTCGTCTTTAGCAATG
AATATTGCTTTCTTCTCTTTCTTAGCACATATTCATGTCTGGTCACTTTAACGCAGTGCT
ACCGTCTGAGACGTGTCGTACTAAGCCTGTGCTGACGGGCTAGCAACCTGTATCACCAAA
GCCCACTTCTTTCCCAGATTCAGAATTGCTGGTTCAACTGCTAAGTANG
3' Read Nucleotide Sequence
>OriGene 3' genomic read for NM_006203 unedited
GTTCATACTGTTGCATATTGCCCACNAAGCTGCTCAAACAAAGATTGGAATTCATCTGTA
AAAATCACTACATGTAACGTAGGAGACAAGAAAAATATTAATGACAGAAGATCTGCGAAC
ATGATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGAC
AATGGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCCAGATCCGGNNCT
ATTCTNCNAGCANATTTTGTCCACAGTCAACGACGGNAGTCCCTTCTGTATCGATCCGAC
AGCGATTATGACCTCTCTCCAAAGTCTATGTCCCNGGACTCCTNCATTTGCAGTGATATA
CCCGAGAATGACTTGAGTGGGACTCCATTTGCCTCAGGTCTGGGCCATCTTGCAAACTGT
ACAAAACAACTTTTGCTGCTTAACCTAATTTGCAGATCGGCCCCCTGGCCAAGATCACCC
TTGTGCCAACCACCCTCCTTTAAACAAAGCCCCCTTAACAAAGAGGCCCTCCCCAAAACG
GGCCCCCCGAACCCCTGAAAAACCTGACTTGGGTTTTGGCCCCCTTTTGAAACCCTTAAA
ACAGACCCCTCCTCCGTGGAAAAGGGCCCCCCCCAATTTTTTAAAAGAGGGGTTTTATTG
GGGGGCCCCCCCCCCCCCTTCTTTAAAAAAAAGGGGGGCCCGGGGGAAAAAAAAGGGGGC
GGAGATTTTTATTATTACAACCACTTTTTTTTAATAATAACACCCCGAGAGGGGGGGAGA
AACTCTTTTCTCTCCCCCCCCCCCGGGGGGGGGGGGGGAGAAAAAAAAAAACACCCCTCT
TTCTCTCTTCTAATTGGGGGTTGGACAAAAAAATTTTTTCGCCCCCCCCTCTTTTTTTT
Restriction Sites Please inquire |
ACCN NM_006203
Insert Size 7000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006203.5
RefSeq Size 5876 bp
RefSeq ORF 2019 bp
Locus ID 5144
UniProt ID Q08499
Cytogenetics 5q11.2-q12.1
Domains PDEase
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
Summary This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.provided by RefSeq, Sep 2009
Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. Isoform PDE4D3 (also known as isoform 2) is shorter and has a distinct N-terminus, compared to isoform PDE4D4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_006203" in other vectors (6)
SKU Description Size Price
RC212410 PDE4D (Myc-DDK-tagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2 10 ug
$615.00
RC212410L1 Lenti ORF clone of Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2, Myc-DDK-tagged 10 ug
$915.00
RC212410L2 Lenti ORF clone of Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2, mGFP tagged 10 ug
$915.00
RC212410L3 Lenti ORF clone of Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2, Myc-DDK-tagged 10 ug
$915.00
RC212410L4 Lenti ORF clone of Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2, mGFP tagged 10 ug
$915.00
RG212410 PDE4D (tGFP-tagged) - Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2 10 ug
$789.00 $815.00

file-alt Citations

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