PDE4D (NM_006203) Human Untagged Clone

SKU SC310179

PDE4D (untagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PDE4D
• Synonyms: ACRDYS2; DPDE3; HSPDE4D; PDE4DN2; PDE43; STRK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006203 edited; ATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGACAAT; GGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGATCCGGGCTAATT; CTCCAAGCAAATTTTGTCCACAGTCAACGACGGGAGTCCTTCCTGTATCGATCCGACAGC; GATTATGACCTCTCTCCAAAGTCTATGTCCCGGAACTCCTCCATTGCCAGTGATATACAC; GGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCTTGGCCAGTCTGCGAACTGTACGA; AACAACTTTGCTGCATTAACTAATTTGCAAGATCGAGCACCTAGCAAAAGATCACCCATG; TGCAACCAACCATCCATCAACAAAGCCACCATAACAGAGGAGGCCTACCAGAAACTGGCC; AGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTACAGACCAGG; CACTCCGTCAGTGAGATGGCCTCCAACAAGTTTAAAAGGATGCTTAATCGGGAGCTCACC; CATCTCTCTGAAATGAGTCGGTCTGGAAATCAAGTGTCAGAGTTTATATCAAACACATTC; TTAGATAAGCAACATGAAGTGGAAATTCCTTCTCCAACTCAGAAGGAAAAGGAGAAAAAG; AAAAGACCAATGTCTCAGATCAGTGGAGTCAAGAAATTGATGCACAGCTCTAGTCTGACT; AATTCAAGTATCCCAAGGTTTGGAGTTAAAACTGAACAAGAAGATGTCCTTGCCAAGGAA; CTAGAAGATGTGAACAAATGGGGTCTTCATGTTTTCAGAATAGCAGAGTTGTCTGGTAAC; CGGCCCTTGACTGTTATCATGCACACCATTTTTCAGGAACGGGATTTATTAAAAACATTT; AAAATTCCAGTAGATACTTTAATTACATATCTTATGACTCTCGAAGACCATTACCATGCT; GATGTGGCCTATCACAACAATATCCATGCTGCAGATGTTGTCCAGTCTACTCATGTGCTA; TTATCTACACCTGCTTTGGAGGCTGTGTTTACAGATTTGGAGATTCTTGCAGCAATTTTT; GCCAGTGCAATACATGATGTAGATCATCCTGGTGTGTCCAATCAATTTCTGATCAATACA; AACTCTGAACTTGCCTTGATGTACAATGATTCCTCAGTCTTAGAGAACCATCATTTGGCT; GTGGGCTTTAAATTGCTTCAGGAAGAAAACTGTGACATTTTCCAGAATTTGACCAAAAAA; CAAAGACAATCTTTAAGGAAAATGGTCATTGACATCGTACTTGCAACAGATATGTCAAAA; CACATGAATCTACTGGCTGATTTGAAGACTATGGTTGAAACTAAGAAAGTGACAAGCTCT; GGAGTTCTTCTTCTTGATAATTATTCCGATAGGATTCAGGTTCTTCAGAATATGGTGCAC; TGTGCAGATCTGAGCAACCCAACAAAGCCTCTCCAGCTGTACCGCCAGTGGACGGACCGG; ATAATGGAGGAGTTCTTCCGCCAAGGAGACCGAGAGAGGGAACGTGGCATGGAGATAAGC; CCCATGTGTGACAAGCACAATGCTTCCGTGGAAAAATCACAGGTGGGCTTCATAGACTAT; ATTGTTCATCCCCTCTGGGAGACATGGGCAGACCTCGTCCACCCTGACGCCCAGGATATT; TTGGACACTTTGGAGGACAATCGTGAATGGTACCAGAGCACAATCCCTCAGAGCCCCTCT; CCTGCACCTGATGACCCAGAGGAGGGCCGGCAGGGTCAAACTGAGAAATTCCAGTTTGAA; CTAACTTTAGAGGAAGATGGTGAGTCAGACACGGAAAAGGACAGTGGCAGTCAAGTGGAA; GAAGACACTAGCTGCAGTGACTCCAAGACTCTTTGTACTCAAGACTCAGAGTCTACTGAA; ATTCCCCTTGATGAACAGGTTGAAGAGGAGGCAGTAGGGGAAGAAGAGGAAAGCCAGCCT; GAAGCCTGTGTCATAGATGATCGTTCTCCTGACACGTAA;
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_006203 unedited; CTACATGTAAGGACTCCCGTCTGTGACTGTGGACAAAATTTGCTTGGACAATTAACCCGG; ATCCTGGGCTGCTCATGGGATCCAAGGGACTCCGTCCCGCAAATGTGCCATTGTCCACAT; CAAAACATATCCAGGAATGCCTTCTAGAGGGAAAATTATTCACGTGCATCATGTTCCCAT; ATCTTCTGTCATTAATATTTTTCTTGTCTCCTACGTTACATGTAGTGATTTTTACAGATG; AATTCCAACTCTCTTGTTTGAGCAACTATCGTGCGCAATTATTGCAACACGTATTGAATC; CCAAAAAAAAAAAAAAAATCTCCCCCCACCAATAAAAACAAATAGCACTTACAAAAGGCC; TTCCATGATTTGAAAAAACAAACATCACAGAACACTGCAGGTATGGATCCATCCATTTAG; GTTCCACTATTTAGTCTACATACTCAAATAACCAGTTCAACTAATAATCCTAACTGGCGG; CCAATATTGTCCTGGAAAAATCTAACCGCCTTGAGTGTGAATTAATGCATCCTTAACGAA; GGACTCTGGAATGTGGGAAGAGAGGAGTCAAAAGACAGTTTCTTGATATCCTTGCTAAAC; AAGTGTTCGCATGGAAATGGAAAACTTCAGGTTGCTTTGCCATGCTCGTCTTTAGCAATG; AATATTGCTTTCTTCTCTTTCTTAGCACATATTCATGTCTGGTCACTTTAACGCAGTGCT; ACCGTCTGAGACGTGTCGTACTAAGCCTGTGCTGACGGGCTAGCAACCTGTATCACCAAA; GCCCACTTCTTTCCCAGATTCAGAATTGCTGGTTCAACTGCTAAGTANG;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_006203 unedited; GTTCATACTGTTGCATATTGCCCACNAAGCTGCTCAAACAAAGATTGGAATTCATCTGTA; AAAATCACTACATGTAACGTAGGAGACAAGAAAAATATTAATGACAGAAGATCTGCGAAC; ATGATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGAC; AATGGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCCAGATCCGGNNCT; ATTCTNCNAGCANATTTTGTCCACAGTCAACGACGGNAGTCCCTTCTGTATCGATCCGAC; AGCGATTATGACCTCTCTCCAAAGTCTATGTCCCNGGACTCCTNCATTTGCAGTGATATA; CCCGAGAATGACTTGAGTGGGACTCCATTTGCCTCAGGTCTGGGCCATCTTGCAAACTGT; ACAAAACAACTTTTGCTGCTTAACCTAATTTGCAGATCGGCCCCCTGGCCAAGATCACCC; TTGTGCCAACCACCCTCCTTTAAACAAAGCCCCCTTAACAAAGAGGCCCTCCCCAAAACG; GGCCCCCCGAACCCCTGAAAAACCTGACTTGGGTTTTGGCCCCCTTTTGAAACCCTTAAA; ACAGACCCCTCCTCCGTGGAAAAGGGCCCCCCCCAATTTTTTAAAAGAGGGGTTTTATTG; GGGGGCCCCCCCCCCCCCTTCTTTAAAAAAAAGGGGGGCCCGGGGGAAAAAAAAGGGGGC; GGAGATTTTTATTATTACAACCACTTTTTTTTAATAATAACACCCCGAGAGGGGGGGAGA; AACTCTTTTCTCTCCCCCCCCCCCGGGGGGGGGGGGGGAGAAAAAAAAAAACACCCCTCT; TTCTCTCTTCTAATTGGGGGTTGGACAAAAAAATTTTTTCGCCCCCCCCTCTTTTTTTT;
• Restriction Sites: Please inquire
• ACCN: NM_006203
• Insert Size: 7000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006203.3, NP_006194.2
• RefSeq Size: 5876 bp
• RefSeq ORF: 2022 bp
• Locus ID: 5144
• UniProt ID: Q08499
• Cytogenetics: 5q11.2-q12.1
• Domains: PDEase
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Summary: This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]; Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. Isoform PDE4D3 (also known as isoform 2) is shorter and has a distinct N-terminus, compared to isoform PDE4D4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.