DGKZ (NM_003646) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | DGKZ |
| Synonyms | DAGK5; DAGK6; DGK-ZETA; hDGKzeta |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_003646 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003646.3, NP_003637.2 |
| RefSeq Size | 3659 bp |
| RefSeq ORF | 2790 bp |
| Locus ID | 8525 |
| UniProt ID | Q13574 |
| Cytogenetics | 11p11.2 |
| Domains | ANK, DAGKa, DAGKc, DAG_PE-bind |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Summary | The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. provided by RefSeq, Nov 2010 Transcript Variant: This variant (2) has an alternate 5' sequence including the coding region and extra nts CAG at an alternate splice site in the CDS, compared to variant 4. The resulting isoform (2) has a shorter and distinct N-terminus and an internal additional aa, compared to isoform 4. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003646" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205327 | DGKZ (Myc-DDK-tagged)-Human diacylglycerol kinase, zeta 104kDa (DGKZ), transcript variant 2 | 10 ug |
$851.00
|
|
| RC205327L3 | Lenti ORF clone of Human diacylglycerol kinase, zeta 104kDa (DGKZ), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,151.00
|
|
| RC205327L4 | Lenti ORF clone of Human diacylglycerol kinase, zeta 104kDa (DGKZ), transcript variant 2, mGFP tagged | 10 ug |
$1,151.00
|
|
| RG205327 | DGKZ (tGFP-tagged) - Human diacylglycerol kinase, zeta 104kDa (DGKZ), transcript variant 2 | 10 ug |
$789.00
$1,051.00
|
|
Citations
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