PAPP A (PAPPA) (NM_002581) Human Untagged Clone
$2,088.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | PAPP A |
| Synonyms | ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_002581 |
| Insert Size | 6800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain 2 SNPs compared with NM_002581.3. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002581.3, NP_002572.2 |
| RefSeq Size | 11025 bp |
| RefSeq ORF | 4884 bp |
| Locus ID | 5069 |
| UniProt ID | Q13219 |
| Cytogenetics | 9q33.1 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| Summary | This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002581" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209767 | PAPPA (Myc-DDK-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) | 10 ug |
$2,087.00
|
|
| RC209767L1 | Lenti-ORF clone of PAPPA (Myc-DDK-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) | 10 ug |
$2,387.00
|
|
| RC209767L2 | Lenti-ORF clone of PAPPA (mGFP-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) | 10 ug |
$2,387.00
|
|
| RC209767L3 | Lenti-ORF clone of PAPPA (Myc-DDK-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) | 10 ug |
$2,387.00
|
|
| RC209767L4 | Lenti-ORF clone of PAPPA (mGFP-tagged)-Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) | 10 ug |
$2,387.00
|
|
| RG209767 | PAPPA (tGFP-tagged) - Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA) | 10 ug |
$2,287.00
|
|
Citations
MiR-490-3p modulates the proliferation of vascular smooth muscle cells induced by ox-LDL through targeting PAPP-A
Sun, Chen, Cao et al
Cardiovasc. Res. (2013)
Sun, Chen, Cao et al
Cardiovasc. Res. (2013)
Cited by 79
AI Summary: In this study, OriGene's PAPP-A siRNA and PAPP-A expression vector were used to manipulate PAPP-A levels in vascular smooth muscle cells, enabling the authors to investigate the regulatory role of miR-490-3p in ox-LDL-induced cell proliferation.
Snippet: 2.2 Vectors, cell culture, and treatment. The siRNA and expression vector of PAPP-A were purchased from Origene. The mutation (MUT) and wild-type (WT) vector of miR-490-3p sponge were constructed by GenePharma (Shanghai, China).
MiR-490-3p modulates proliferation of vascular smooth muscle cells induced by ox-LDL through targeting PAPP-A
Sun, Chen, Cao et al
Cardiovascular Research Advance Access (2013)
Sun, Chen, Cao et al
Cardiovascular Research Advance Access (2013)
Snippet: Chinese PLA General Hospital. Vectors, cell culture and treatment The siRNA and expression vector of PAPP-A were purchased from Origene. The mutation and wide type vector of miR-490-3p sponge were constructed by GenePharma (Shanghai, China). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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