LRRC33 (NRROS) (NM_198565) Human Untagged Clone

SKU SC309386

LRRC33 (untagged)-Human leucine rich repeat containing 33 (LRRC33)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LRRC33
• Synonyms: ELLP3030; GARPL1; LRRC33; SENEBAC; UNQ3030
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198565 edited; GGTAGCCCAGCCCCGCGTTCGGCTGCTCTCGAGGAGGCCGGAGCCCCCGGAGACGATGCG; CCCCGCGCAGCCGCCTGCGCCTGCGGGAGCCGGCTGCCCTTGAGATGGAGTTGCTGCCTC; TTTGGCTCTGCCTGGGTTTTCACTTCCTGACCGTGGGCTGGAGGAACAGAAGCGGAACAG; CCACAGCAGCCTCCCAAGGAGTCTGCAAGTTGGTGGGTGGAGCCGCTGACTGCCGAGGGC; AGAGCCTCGCTTCGGTGCCCAGCAGCCTCCCGCCCCACGCCCGGATGCTCACCCTGGATG; CCAACCCTCTCAAGACCCTGTGGAATCACTCCCTCCAGCCTTACCCTCTCCTGGAGAGCC; TCAGCCTGCACAGCTGCCACCTGGAGCGCATCAGCCGCGGCGCCTTCCAGGAGCAAGGTC; ACCTGCGCAGCCTGGTACTGGGGGACAACTGCCTCTCAGAGAACTACGAAGAGACGGCAG; CCGCCCTCCACGCCCTGCCGGGCCTGCGGAGGCTGGACTTGTCAGGAAACGCCCTGACGG; AGGACATGGCAGCGCTCATGCTCCAGAACCTCTCCTCGCTGCGGTCCGTGTCCCTGGCGG; GGAACACCATCATGCGGCTGGACGACTCCGTCTTCGAGGGCCTGGAGCGTCTCCGGGAGC; TGGATCTGCAGAGGAACTACATCTTCGAGATCGAGGGCGGCGCTTTCGACGGCCTGGCTG; AGCTGAGGCACCTCAACCTGGCCTTCAACAACCTCCCCTGCATCGTGGACTTCGGGCTCA; CGCGGCTGCGGGTCCTCAACGTCAGCTACAACGTCCTGGAGTGGTTCCTCGCGACCGGGG; GAGAGGCTGCCTTCGAGCTGGAGACGCTGGACCTGTCTCACAACCAGCTGCTGTTCTTCC; CGCTGCTGCCCCAGTACAGCAAGTTGCGGACCCTCCTGCTGCGCGACAACAACATGGGCT; TCTACCGGGACCTGTACAACACCTCGTCGCCGAGGGAGATGGTGGCCCAGTTCCTCCTCG; TGGACGGCAACGTGACCAACATCACCACCGTCAGCCTCTGGGAAGAATTCTCCTCCAGCG; ACCTCGCAGATCTCCGCTTCCTGGACATGAGCCAGAACCAGTTCCAGTACCTGCCAGACG; GCTTCCTGAGGAAAATGCCTTCCCTCTCCCACCTGAACCTCCACCAGAATTGCCTGATGA; CGCTTCACATTCGGGAGCACGAGCCCCCCGGAGCGCTCACCGAGCTGGACCTGAGCCACA; ACCAGCTGTCGGAGCTGCACCTGGCTCCGGGGCTGGCCAGCTGCCTGGGCAGCCTGCGCT; TGTTCAACCTGAGCTCCAACCAGCTCCTGGGCGTCCCCCCTGGCCTCTTCGCCAATGCTA; GGAACATCACTACACTTGACATGAGCCACAATCAGATCTCACTTTGTCCCCTGCCAGCTG; CCTCGGACCGGGTGGGCCCCCCTAGCTGTGTGGATTTCAGGAATATGGCATCTTTAAGGA; GCCTGTCTCTGGAGGGCTGTGGCCTGGGGGCATTGCCAGACTGCCCATTCCAAGGGACCT; CCCTGACCTACTTAGACCTCTCAAGCAACTGGGGGGTTCTGAATGGGAGCCTCGCCCCAC; TCCAGGATGTTGCCCCCATGTTACAGGTCCTGTCTCTCAGGAACATGGGCCTCCACTCCA; GCTTTATGGCGTTGGACTTCTCTGGGTTTGGGAATCTCAGGGACTTAGATCTGTCGGGGA; ATTGCTTGACCACCTTCCCAAGGTTTGGGGGCAGCCTGGCCCTGGAGACCCTGGATCTCC; GTAGAAACTCGCTCACAGCCCTTCCCCAGAAGGCTGTGTCTGAGCAGCTCTCGAGAGGTC; TGCGGACCATCTACCTCAGTCAGAATCCATATGACTGCTGTGGGGTGGATGGCTGGGGGG; CCCTGCAGCATGGGCAGACGGTGGCCGACTGGGCCATGGTCACCTGCAACCTCTCCTCCA; AGATCATCCGCGTGACGGAGCTGCCCGGAGGTGTGCCTCGGGACTGCAAGTGGGAGCGGC; TGGACCTGGGCCTGCTCTACCTCGTGCTCATCCTCCCCAGCTGCCTCACCCTGCTGGTGG; CCTGCACTGTCATCGTCCTCACTTTTAAGAAGCCTCTGCTTCAGGTCATCAAGAGCCGCT; GCCACTGGTCCTCCGTTTACTGACCTGGCTGTGTGCCAAGACTCGAAATTCGGTCCGCAC; ACAACAGGACACTTTCTCTGCCAGCTTTCAAGATGTGATGCAGAGGCCAAGTCTGACGAA; TTGAAGTTTCAATTAAAATTTAATATGTTTCCATTCCTCATCGCCCACCCCACCCCCGCC; CCCACCACCGCCCAAGTTCTTTTTCCATCATTATAATTCATCCTTATTATCTTGGTAAAA; TATTTATTAAGTGACTTTTTCAGAAATAAAAGGCAACGTGTCTCATAAATATTTTTTAAA; TTAAAAAAAAAAAAAAAAAAAAAAAAAA;
• Restriction Sites: Please inquire
• ACCN: NM_198565
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_198565.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198565.1, NP_940967.1
• RefSeq Size: 2488 bp
• RefSeq ORF: 2079 bp
• Locus ID: 375387
• UniProt ID: Q86YC3
• Cytogenetics: 3q29
• Protein Families: Transmembrane
• Summary: Key regulator of transforming growth factor beta-1 (TGFB1) specifically required for microglia function in the nervous system (By similarity). Required for activation of latent TGF-beta-1 in macrophages and microglia: associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGFB1, and regulates integrin-dependent activation of TGF-beta-1 (By similarity). TGF-beta-1 activation mediated by LRRC33/NRROS is highly localized: there is little spreading of TGF-beta-1 activated from one microglial cell to neighboring microglia, suggesting the existence of localized and selective activation of TGF-beta-1 by LRRC33/NRROS (By similarity). Indirectly plays a role in Toll-like receptor (TLR) signaling: ability to inhibit TLR-mediated NF-kappa-B activation and cytokine production is probably a consequence of its role in TGF-beta-1 signaling (PubMed:23545260).[UniProtKB/Swiss-Prot Function]