DKK4 (NM_014420) Human Untagged Clone

SKU SC304098

DKK4 (untagged)-Human dickkopf homolog 4 (Xenopus laevis) (DKK4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DKK4
• Synonyms: DKK-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014420 edited; CTGCCAGCTTAGTGGAAGCTCTGCTCTGGGTGGAGAGCAGCCTCGCTTTGGTGACGCACA; GTGCTGGGACCCTCCAGGAGCCCCGGGATTGAAGGATGGTGGCGGCCGTCCTGCTGGGGC; TGAGCTGGCTCTGCTCTCCCCTGGGAGCTCTGGTCCTGGACTTCAACAACATCAGGAGCT; CTGCTGACCTGCATGGGGCCCGGAAGGGCTCACAGTGCCTGTCTGACACGGACTGCAATA; CCAGAAAGTTCTGCCTCCAGCCCCGCGATGAGAAGCCGTTCTGTGCTACATGTCGTGGGT; TGCGGAGGAGGTGCCAGCGAGATGCCATGTGCTGCCCTGGGACACTCTGTGTGAACGATG; TTTGTACTACGATGGAAGATGCAACCCCAATATTAGAAAGGCAGCTTGATGAGCAAGATG; GCACACATGCAGAAGGAACAACTGGGCACCCAGTCCAGGAAAACCAACCCAAAAGGAAGC; CAAGTATTAAGAAATCACAAGGCAGGAAGGGACAAGAGGGAGAAAGTTGTCTGAGAACTT; TTGACTGTGGCCCTGGACTTTGCTGTGCTCGTCATTTTTGGACGAAAATTTGTAAGCCAG; TCCTTTTGGAGGGACAGGTCTGCTCCAGAAGAGGGCATAAAGACACTGCTCAAGCTCCAG; AAATCTTCCAGCGTTGCGACTGTGGCCCTGGACTACTGTGTCGAAGCCAATTGACCAGCA; ATCGGCAGCATGCTCGATTAAGAGTATGCCAAAAAATAGAAAAGCTATAAATATTTCAAA; ATAAAGAAGAATCCACATTGCAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014420 unedited; GGTCAGTTAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCGCCCTTCTGCCAGCT; TAGTGGAAGCTCTGCTCTGGGTGGAGAGCAGCCTCGCTTTGGTGACGCACAGTGCTGGGA; CCCTCCAGGAGCCCCGGGATTGAAGGATGGTGGCGGCCGTCCTGCTGGGGCTGAGCTGGC; TCTGCTCTCCCCTGGGAGCTCTGGTCCTGGACTTCAACAACATCAGGAGCTCTGCTGACC; TGCATGGGGCCCGGAAGGGCTCACAGTGCCTGTCTGACACGGACTGCAATACCAGAAAGT; TCTGCCTCCAGCCCCGCGATGAGAAGCCGTTCTGTGCTACATGTCGTGGGTTGCGGAGGA; GGTGCCAGCGAGATGCCATGTGCTGCCCTGGGACACTCTGTGTGAACGATGTTTGTACTA; CGATGGAAGATGCAACCCCAATATTAGAAAGGCAGCTTGATGAGCAAGATGGCACACATG; CAGAAGGAACAACTGGGCACCCAGTCCAGGAAAACCAACCCAAAAGGAAGCCAAGTATTA; AGAAATCACAAGGCAGGAAGGGACAAGAGGGAGAAAGTTGTCTGAGAACTTTTGACTGTG; GCCCTGGACTTTGCTGTGCTCGTCATTTTTGGACGAAAATTTGTAAGCCAGTCCTTTTGG; AGGGACAGGTCTGCTCCAGAAGAGGGCATAAAGACACTGCTCAAGCTCCAGAAATCTTCC; AGCGTTGCGACTGTGGCCCTGGACTACTGTGTCGAAGCCAATTGACCAGCAATCGGCAGC; ATGCTCGATTAAGAAGTATGCCAAAAATAGAAAAGCCTATAATATTTTCANAATANAGAA; GAATCCACATTGCANAGGGCGAATTCAGATCTGGTACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014420 unedited; CATTGGNATGGCACTTCCAGGNCCAGNAAAGCACTGGGGCAGGGTCACAGGGATGCCACC; CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGAT; ATCGGTACCAGATCTGAATTCGCCCTTTGCAATGTGGATTCTTCTTTATTTTGAAATATT; TATAGCTTTTCTATTTTTTGGCATACTCTTAATCGAGCATGCTGCCGATTGCTGGTCAAT; TGGCTTCGACACAGTAGTCCAGGGCCACAGTCGCAACGCTGGAAGATTTCTGGAGCTTGA; GCAGTGTCTTTATGCCCTCTTCTGGAGCAGACCTGTCCCTCCAAAAGGACTGGCTTACAA; ATTTTCGTCCAAAAATGACGAGCACAGCAAAGTCCAGGGCCACAGTCAAAAGTTCTCAGA; CAACTTTCTCCCTCTTGTCCCTTCCTGCCTTGTGATTTCTTAATACTTGGCTTCCTTTTG; GGTTGGTTTTCCTGGACTGGGTGCCCAGTTGTTCCTTCTGCATGTGTGCCATCTTGCTCA; TCAAGCTGCCTTTCTAATATTGGGGTTGCATCTTCCATCGTAGTACANACATCGTTCACA; CAGAGTGTCCCAGGGCAGCACATGGCATCTCGCTGGCACCTCCTCCGCAACCCACGACAT; GTAGCACAGAACGGCTTCTCATCGCGGNGCTGGAGGCAGAACTTTCTGGGTATGCAGTCC; GTGTCAGACAGGCACTGTGAGCCCCTTCGGGCCCCNATGCAGTCAGCAGAAGCTCTGATG; TTGNTGAAAGTCAGGACCAGAGCTCCCAGGGGAGAGCAGAGCCAGCTCAGCCCCAGCAGG; ACGGCCGCCACATCCTTCA;
• Restriction Sites: Please inquire
• ACCN: NM_014420
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014420.2, NP_055235.1
• RefSeq Size: 835 bp
• RefSeq ORF: 675 bp
• Locus ID: 27121
• UniProt ID: Q9UBT3
• Cytogenetics: 8p11.21
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway
• Summary: This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]