HEY2 (NM_012259) Human Untagged Clone

SKU SC303947

HEY2 (untagged)-Human hairy/enhancer-of-split related with YRPW motif 2 (HEY2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HEY2
• Synonyms: bHLHb32; CHF1; GRIDLOCK; GRL; HERP1; HESR2; HRT2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012259 edited; GGGGAGGGAGGAGGCGGGCGTCAGGGTGCTGCGCCCCGCTCGGCGTCCGAGCTTCCGGCC; GGGCTGTGCCCCGCGCGGTCTTCGCCGGGATGAAGCGCCCCTGCGAGGAGACGACCTCCG; AGAGCGACATGGACGAGACCATCGACGTGGGGAGCGAGAACAATTACTCGGGGCAAAGTA; CTAGCTCTGTGATTAGATTGAATTCTCCAACAACAACATCTCAGATTATGGCAAGAAAGA; AAAGGAGAGGGATTATAGAGAAAAGGCGTCGGGATCGGATAAATAACAGTTTATCTGAGT; TGAGAAGACTTGTGCCAACTGCTTTTGAAAAACAAGGATCTGCAAAGTTAGAAAAAGCTG; AAATATTGCAAATGACAGTGGATCATTTGAAGATGCTTCAGGCAACAGGGGGTAAAGGCT; ACTTTGACGCACACGCTCTTGCCATGGACTTCATGAGCATAGGATTCCGAGAGTGCCTAA; CAGAAGTTGCGCGGTACCTGAGCTCCGTGGAAGGCCTGGACTCCTCGGATCCGCTGCGGG; TGCGGCTTGTGTCTCATCTCAGCACTTGCGCCACCCAGCGGGAGGCGGCGGCCATGACAT; CCTCCATGGCCCACCACCATCATCCGCTCCACCCGCATCACTGGGCCGCCGCCTTCCACC; ACCTGCCCGCAGCCCTGCTCCAGCCCAACGGCCTCCATGCCTCAGAGTCAACCCCTTGTC; GCCTCTCCACAACTTCAGAAGTGCCTCCTGCCCACGGCTCTGCTCTCCTCACGGCCACGT; TTGCCCATGCGGATTCAGCCCTCCGAATGCCATCCACGGGCAGCGTCGCCCCCTGCGTGC; CACCTCTCTCCACCTCTCTCTTGTCCCTCTCTGCCACCGTCCACGCCGCAGCCGCAGCAG; CCACCGCGGCTGCACACAGCTTCCCTCTGTCCTTCGCGGGGGCATTCCCCATGCTTCCCC; CAAACGCAGCAGCAGCAGTGGCCGCGGCCACAGCCATCAGCCCGCCCTTGTCAGTATCAG; CCACGTCCAGTCCTCAGCAGACCAGCAGTGGAACAAACAATAAACCTTACCGACCCTGGG; GGACAGAAGTTGGAGCTTTTTAAATTTTTCTTGAACTTCTTGCAATAGTAACTGAATGTC; CTCCATTTCAGAGTCAGCTTAAAACCTCTGCACCCTGAAGGTAGCCATACAGATGCCGAC; AGATCCACAAAGGAACAATAAAGCTATTTGAGACACAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012259 unedited; ATAGGCGGCCGCGNAATTCGCACGAGGGGNGAGGAGGAGGCGGGCGTCAGGGTGCTGCGC; CCCGCTCGGCGTCCGAGCTTCCGGCCGGGCTGTGCCCCGCGCGGTCTTCGCCGGGATGAA; GCGCCCCTGCGAGGAGACGACCTCCGAGAGCGACATGGACGAGACCATCGACGTGGGGAG; CGAGAACAATTACTCGGGGCAAAGTACTAGCTCTGTGATTAGATTGAATTCTCCAACAAC; AACATCTCAGATTATGGCAAGAAAGAAAAGGAGAGGGATTATAGAGAAAAGGCGTCGGGA; TCGGATAAATAACAGTTTATCTGAGTTGAGAAGACTTGTGCCAACTGCTTTTGAAAAACA; AGGATCTGCAAAGTTAGAAAAAGCTGAAATATTGCAAATGACAGTGGATCATTTGAAGAT; GCTTCAGGCAACAGGGGGTAAAGGCTACTTTGACGCACACGCTCTTGCCATGGACTTCAT; GAGCATAGGATTCCGAGAGTGCCTAACAGAAGTTGCGCGGTACCTGAGCTCCGTGGAAGG; CCTGGACTCCCTCGATCCGCTGCGGGTGCGGCTTGTGTCTCATCTCAGCACTTGCGCCAC; CCAGCGGGAGGCGGCGGCCATGACATCCTNCATGGCCCACCACCATCATCCGCTCCACCC; GCATCACTGGGCCCGCGCCTTTCACCACCTGCCCGCAGCCCTGCTCCAGCCCAACGGCCT; CCATGCCTCAGAGTCAACCCCTGTCGCCTTTCCACAACTCAGAAGTGCCTCCTGCCCACG; GTTCTGCTTTTCCTACGGGCCCGTTTTGCCCTTGCGGATTCAGCCCCTCGAATGGCATCC; CCGGGCAG;
• Restriction Sites: Please inquire
• ACCN: NM_012259
• Insert Size: 1265 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012259.2, NP_036391.1
• RefSeq Size: 2672 bp
• RefSeq ORF: 1014 bp
• Locus ID: 23493
• UniProt ID: Q9UBP5
• Cytogenetics: 6q22.31
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]