GPA33 (NM_005814) Human Untagged Clone

SKU SC303695

GPA33 (untagged)-Human glycoprotein A33 (transmembrane) (GPA33)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GPA33
• Synonyms: A33
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005814 edited; CTACCCCTTTGTGAGCAGTCTAGGACTTTGTACACCTGTTAAGTAGGGAGAAGGCAGGGG; AGGTGGCTGGTTTAAGGGGAACTTGAGGGAAGTAGGGAAGACTCCTCTTGGGACCTTTGG; AGTAGGTGACACATGAGCCCAGCCCCAGCTCACCTGCCAATCCAGCTGAGGAGCTCACCT; GCCAATCCAGCTGAGGCTGGGCAGAGGTGGGTGAGAAGAGGGAAAATTGCAGGGACCTCC; AGTTGGGCCAGGCCAGAAGCTGCTGTAGCTTTAACCAGACAGCTCAGACCTGTCTGGAGG; CTGCCAGTGACAGGTTAGGTTTAGGGCAGAGAAGAAGCAAGACCATGGTGGGGAAGATGT; GGCCTGTGTTGTGGACACTCTGTGCAGTCAGGGTGACCGTCGATGCCATCTCTGTGGAAA; CTCCGCAGGACGTTCTTCGGGCTTCGCAGGGAAAGAGTGTCACCCTGCCCTGCACCTACC; ACACTTCCACCTCCAGTCGAGAGGGACTTATTCAATGGGATAAGCTCCTCCTCACTCATA; CGGAAAGGGTGGTCATCTGGCCGTTTTCAAACAAAAACTACATCCATGGTGAGCTTTATA; AGAATCGCGTCAGCATATCCAACAATGCTGAGCAGTCCGATGCCTCCATCACCATTGATC; AGCTGACCATGGCTGACAACGGCACCTACGAGTGTTCTGTCTCGCTGATGTCAGACCTGG; AGGGCAACACCAAGTCACGTGTCCGCCTGTTGGTCCTCGTGCCACCCTCCAAACCAGAAT; GCGGCATCGAGGGAGAGACCATAATTGGGAACAACATCCAGCTGACCTGCCAATCAAAGG; AGGGCTCACCAACCCCTCAGTACAGCTGGAAGAGGTACAACATCCTGAATCAGGAGCAGC; CCCTGGCCCAGCCAGCCTCAGGTCAGCCTGTCTCCCTGAAGAATATCTCCACAGACACAT; CGGGTTACTACATCTGTACCTCCAGCAATGAGGAGGGGACGCAGTTCTGCAACATCACGG; TGGCCGTCAGATCTCCCTCCATGAACGTGGCCCTGTATGTGGGCATCGCGGTGGGCGTGG; TTGCAGCCCTCATTATCATTGGCATCATCATCTACTGCTGCTGCTGCCGAGGGAAGGACG; ACAACACTGAAGACAAGGAGGATGCAAGGCCGAACCGGGAAGCCTATGAGGAGCCACCAG; AGCAGCTAAGAGAACTTTCCAGAGAGAGGGAGGAGGAGGATGACTACAGGCAAGAAGAGC; AGAGGAGCACTGGGCGTGAATCCCCGGACCACCTCGACCAGTGACAGGCCAGCAGCAGAG; GGCGGCGGAGGAAGGGTTAGGGGTTCATTCTCCCGCTTCCTGGCCTCCCTTCTCCTTTCT; AAGCCCTGTTCTCCTGTCCCTCCATCCCAGACATTGATGGGGACATTTCTTCCCCAGTGT; CAGCTGTGGGGAACATGGCTGGCCTGGTAAGGGGGTCCCTGTGCTGATCCTGCTGACCTC; ACTGTCCTGTGAAGTAACCCCTCCTGGCTGTGACACCTGGTGCGGGCCTGGCCCTCACTC; AAGACCAGGCTGCAGCCTCCACTTCCCTCGTAGTTGGCAGGAGCTCCTGGAAGCACAGCG; CTGAGCATGGGGCGCTCCCACTCAGAACTCTCCAGGGAGGCGATGCCAGCCTTGGGGGGT; GGGGGCTGTCCTGCTCACCTGTGTGCCCAGCACCTGGAGGGGCACCAGGTGGAGGGTTTA; CACTCCACACATCTTTCTTGAATGAATGAAAGAATAAGTGAGTATGCTTGGGCCCTGCAT; TGGCCTGGCCTCCAGCTCCCACTCCCTTTCCAACCTCACTTCCCGTAGCTGCCAGTATGT; TCCAAACCCTCCTGGGAAGGCCACCTCCCACTCCTGCTGCACAGGCCCTGGGGAGCTTTT; GCCCACACACTTTCCATCTCTGCCTGTCAATATCGTACCTGTCCCTCCAGGCCCATCTCA; AATCACAAGGATTTCTCTAACCCTATCCTAATTGTCCACATACGTGGAAACAATCCTGTT; ACTCTGTCCCACGTCCAATCATGGGCCACAAGGCACAGTCTTCTGAGCGAGTGCTCTCAC; TGTATTAGAGCGCCAGCTCCTTGGGGCAGGGCCTGGGCCTCATGGCTTTTGCTTTCCCTG; AAGCCCTAGTAGCTGGCGCCCATCCTAGTGGGCACTTAAGCTTAATTGGGGAAACTGCTT; TGATTGGTTGTGCCTTCCCTTCTCTGGTCTCCTTGAGATGATCGTAGACACAGGGATGAT; TCCCACCCAAACCCACGTATTCATTCAGTGAGTTAAACACGAATTGATTTAAAGTGAACA; CACACAAGGGAGCTTGCTTGCAGATGGTCTGAGTTCTTGTGTCCTGGTAATTCCTCTCCA; GGCCAGAATAATTGGCATGTCTCCTCAACCCACATGGGGTTCCTGGTTGTTCCTGCATCC; CGATACCTCAGCCCTGGCCCTGCCCAGCCCATTTGGGCTCTGGTTTTCTGGTGGGGCTGT; CCTGCTGCCCTCCCACAGCCTCCTTCTGTTTGTCGAGCATTTCTTCTACTCTTGAGAGCT; CAGGCAGCGTTAGGGCTGCTTAGGTCTCAT;
• Restriction Sites: Please inquire
• ACCN: NM_005814
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_005814.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005814.1, NP_005805.1
• RefSeq Size: 2793 bp
• RefSeq ORF: 960 bp
• Locus ID: 10223
• UniProt ID: Q99795
• Cytogenetics: 1q24.1
• Protein Families: Druggable Genome, Transmembrane
• Summary: The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008]