EGR3 (NM_004430) Human Untagged Clone

SKU SC303482

EGR3 (untagged)-Human early growth response 3 (EGR3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EGR3
• Synonyms: EGR-3; PILOT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004430 edited; GGAGTGCTATGACCGGCAAACTCGCCGAGAAGCTGCCGGTGACCATGAGCAGTTTGCTAA; ACCAACTGCCTGACAATCTGTACCCCGAGGAGATCCCCAGCGCGCTCAACCTCTTCTCCG; GCAGCAGCGACTCGGTAGTCCATTACAATCAGATGGCTACAGAGAATGTAATGGACATCG; GTCTGACCAACGAGAAGCCCAACCCGGAACTCTCTTACTCCGGCTCCTTCCAGCCAGCCC; CCGGCAACAAGACCGTGACCTACTTGGGAAAGTTCGCCTTCGACTCCCCTTCCAACTGGT; GCCAGGACAACATCATTAGCCTCATGAGCGCCGGCATCTTGGGGGTGCCCCCGGCTTCAG; GGGCGCTCAGCACGCAGACGTCCACGGCCAGCATGGTGCAGCCACCGCAGGGTGACGTGG; AGGCCATGTATCCCGCGCTACCCCCCTACTCCAACTGCGGCGACCTCTACTCAGAGCCCG; TGTCTTTCCACGACCCCCAGGGCAATCCCGGGCTCGCCTATTCCCCCCAGGATTACCAAT; CGGCCAAGCCGGCGTTGGACAGCAATCTCTTCCCCATGATTCCTGACTACAACCTCTACC; ACCACCCCAACGACATGGGCTCCATTCCGGAGCACAAGCCCTTCCAGGGCATGGACCCCA; TCCGGGTCAACCCGCCCCCTATTACCCCTCTGGAGACCATCAAGGCATTCAAAGACAAGC; AGATCCACCCGGGCTTTGGCAGCCTGCCCCAGCCGCCGCTCACCCTCAAGCCCATCCGGC; CCCGCAAGTACCCCAACCGGCCTAGCAAGACACCGCTCCACGAACGGCCCCACGCGTGCC; CGGCCGAGGGCTGCGACCGCCGTTTCAGCCGTTCGGACGAGCTGACCCGGCACCTGCGCA; TCCACACGGGCCACAAGCCCTTCCAGTGCCGGATCTGCATGCGGAGCTTCAGCCGCAGCG; ACCACCTCACCACTCACATCCGCACTCATACGGGCGAGAAGCCCTTTGCCTGCGAGTTCT; GCGGGCGCAAGTTTGCGCGCAGCGACGAGCGCAAGCGCCACGCCAAGATCCACCTCAAGC; AAAAGGAGAAGAAGGCGGAGAAGGGCGGTGCACCCTCTGCATCCTCGGCGCCCCCCGTGT; CGCTGGCCCCCGTGGTCACCACCTGCGCCTGAGGATCGGGCCCCCAGATCCCCACTTTTC; CCCT;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004430 unedited; TTGTAATACGAACTCACTATAGGGCGGCCGCGATTCAAATCTGGTACCGAGCTCGGATCC; ACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGGAGTGCTATGACCGGCAAACTC; GCCGAGAAGCTGCCGGTGACCATGAGCAGTTTGCTAAACCAACTGCCTGACAATCTGTAC; CCCGAGGAGATCCCCAGCGCGCTCAACCTCTTCTCCGGCAGCAGCGACTCGGTAGTCCAT; TACAATCAGATGGCTACAGAGAATGTAATGGACATCGGTCTGACCAACGAGAAGCCCAAC; CCGGAACTCTCTTACTCCGGCTCCTTCCAGCCAGCCCCCGGCAACAAGACCGTGACCTAC; TTGGGAAAGTTCGCCTTCGACTCCCCTTCCAACTGGTGCCAGGACAACATCATTAGCCTC; ATGAGCGCCGGCATCTTGGGGGTGCCCCCGGCTTCAGGGGCGCTCAGCACGCAGACGTCC; ACGGCCAGCATGGTGCAGCCACCGCANGGTGACGTGGAGGCCATGTATCCCGCGCTACCC; CCCTACTCCAAACTGCGCGACCTCTACTCAGAGCCCGTGTCTTTCCACGACCCCCAGGGC; AATCCCGGGCTCGCCTATTCCCCCCCAGATTACCAATCGGCCAAGCCCGCGTTTGACAGC; AATCTCNTCCCCATGATTCCTGACTTACAACCTCTACCACCACCCCAACGACATGGGCTC; CATTTCCGGAGCACAAGCCCCTTCCCAGGCATGGGACCCCATCCCGGGTCAACCCCGCCC; CCCTATTACCCCCTCTTGAAAACCATTCAAGCATTTCAAAGACCAGCAGATCCACCCCGG; GCTTTTGAAAGCCCTGGCCCAAGCCGGCGGTTTAACCTTAAAGCCCATTCGGGCCCGGAA; GTACCCCAAACGGGCCTATCAAAGAAACCGTTCCAGAGACGGCCCCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004430 unedited; CATTGGNGATGGCACTTCCAGGNCCAGNANGAGCACTGGGGNAGGGTCACAGGGATGCCA; CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATGCATGCTCGAGC; GGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTAGGGGAAAAGTGGGGATCTGGG; GGCCCGATCCTCAGGCGCAGGTGGTGACCACGGGGGCCAGCGACACGGGGGGCGCCGAGG; ATGCAGAGGGTGCACCGCCCTTCTCCGCCTTCTTCTCCTTTTGCTTGAGGTGGATCTTGG; CGTGGCGCTTGCGCTCGTCGCTGCGCGCAAACTTGCGCCCGCAGAACTCGCAGGCAAAGG; GCTTCTCGCCCGTATGAGTGCGGATGTGAGTGGTGAGGTGGTCGCTGCGGCTGAAGCTCC; GCATGCAGATCCGGCACTGGAAGGGCTTGTGGCCCGTGTGGATGCGCAGGTGCCGGGTCA; GCTCGTCCGAACGGCTGAAACGGCGGTCGCAGCCCTCGGCCGGGCACGCGTGGGGCCGTT; CGTGGAGCGGTGTCTTGCTAGGCCGGTTGGGGTACTTGCGGGGCCGGATGGGCTTGAGGG; TGAGCGGCGGCTGGGGCAGGCTGCCAAAGCCCGGGTGGATCTGCTTGTCTTTGAATGCCT; TGATGGTCTCCAGAGGGGTAATAGGGGGCGGGTTGACCCGGATGGNGTCCATGCCCTGGA; AGGGCTTGTGCTCCGGAATGGAGCCCATGTCGTTGGGGTGGTGGTAGAGGTTGTAGTCAG; GAATCATGGGGAAGAGATTGCTGTCCAACGCCGGCTTGGCCGATTGGTAATCTGGNGG;
• Restriction Sites: Please inquire
• ACCN: NM_004430
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004430.2, NP_004421.2
• RefSeq Size: 4342 bp
• RefSeq ORF: 1164 bp
• Locus ID: 1960
• UniProt ID: Q06889
• Cytogenetics: 8p21.3
• Summary: This gene encodes a transcriptional regulator that belongs to the EGR family of C2H2-type zinc-finger proteins. It is an immediate-early growth response gene which is induced by mitogenic stimulation. The protein encoded by this gene participates in the transcriptional regulation of genes in controling biological rhythm. It may also play a role in a wide variety of processes including muscle development, lymphocyte development, endothelial cell growth and migration, and neuronal development. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).