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IL5RA (NM_000564) Human Untagged Clone

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SKU
SC300097
IL5RA (untagged)-Human interleukin 5 receptor, alpha (IL5RA), transcript variant 1
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IL5RA
Synonyms CD125; CDw125; HSIL5R3; IL5R
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>SC300097 representing NM_000564.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

ATGATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATACTGCAAGCTGACTTACTT
CCTGATGAAAAGATTTCACTTCTCCCGCCTGTCAATTTCACCATTAAAGTTACTGGTTTGGCTCAAGTT
CTTTTACAATGGAAACCAAATCCTGATCAAGAGCAAAGGAATGTTAATCTAGAATATCAAGTGAAAATA
AACGCTCCAAAAGAAGATGACTATGAAACCAGAATCACTGAAAGCAAATGTGTAACCATCCTCCACAAG
GGCTTTTCAGCAAGTGTGCGGACCATCCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCT
GCTGAACTTCATGCCCCACCAGGGTCTCCTGGAACCTCAATTGTGAATTTAACTTGCACCACAAACACT
ACAGAAGACAATTATTCACGTTTAAGGTCATACCAAGTTTCCCTTCACTGCACCTGGCTTGTTGGCACA
GATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTATGGCTCTTGGACTGAAGAATGCCAAGAA
TACAGCAAAGACACACTGGGGAGAAATATCGCATGCTGGTTTCCCAGGACTTTTATCCTCAGCAAAGGG
CGTGACTGGCTTGCGGTGCTTGTTGACGGCTCCAGCAAGCACTCTGCTATCAGGCCCTTTGATCAGCTG
TTTGCCCTTCACGCCATTGATCAAATAAATCCTCCACTGAATGTCACAGCAGAGATTGAAGGAACTCGT
CTCTCTATCCAATGGGAGAAACCAGTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAAAATA
CACAATACAAGGAATGGATATTTGCAGATAGAAAAATTGATGACCAATGCATTCATCTCAATAATTGAT
GATCTTTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCATGTGCAGAGAGGCAGGGCTCTGG
AGTGAGTGGAGCCAACCTATTTATGTGGGAAATGATGAACACAAGCCCTTGAGAGAGTGGTTTGTCATT
GTGATTATGGCAACCATCTGCTTCATCTTGTTAATTCTCTCGCTTATCTGTAAAATATGTCATTTATGG
ATCAAGTTGTTTCCACCAATTCCAGCACCAAAAAGTAATATCAAAGATCTCTTTGTAACCACTAACTAT
GAGAAAGCTGGGTCCAGTGAGACGGAAATTGAAGTCATCTGTTATATAGAGAAGCCTGGAGTTGAGACC
CTGGAGGATTCTGTGTTTTGA
Restriction Sites SgfI-MluI |
ACCN NM_000564
Insert Size 1263 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000564.2.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000564.2
RefSeq Size 2367 bp
RefSeq ORF 1263 bp
Locus ID 3568
UniProt ID Q01344
Cytogenetics 3p26.2
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
MW 47.7 kDa
Summary The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. provided by RefSeq, Jul 2011
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_000564" in other vectors (4)
SKU Description Size Price
RC211741 IL5RA (Myc-DDK-tagged)-Human interleukin 5 receptor, alpha (IL5RA), transcript variant 1 10 ug
$457.00
RC211741L3 Lenti ORF clone of Human interleukin 5 receptor, alpha (IL5RA), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211741L4 Lenti ORF clone of Human interleukin 5 receptor, alpha (IL5RA), transcript variant 1, mGFP tagged 10 ug
$757.00
RG211741 IL5RA (tGFP-tagged) - Human interleukin 5 receptor, alpha (IL5RA), transcript variant 1 10 ug
$489.00 $657.00

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