RNF111 (NM_017610) Human Untagged Clone

SKU SC128219

RNF111 (untagged)-Human ring finger protein 111 (RNF111)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RNF111
• Synonyms: ARK; hRNF111
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128219 sequence for NM_017610 edited (data generated by NextGen Sequencing); ATGTCTCAATGGACTCCTGAATATAACGAGCTCTACACCTTAAAAGTGGATATGAAGAGT; GAGATTCCTTCTGATGCACCAAAGACACAGGAGAGTCTGAAAGGGATCCTTTTGCATCCA; GAGCCCATTGGGGCAGCCAAAAGTTTTCCTGCAGGAGTTGAGATGATTAATAGTAAAGTG; GGGAATGAATTCTCTCACCTGTGTGATGATTCTCAAAAGCAAGAGAAGGAAATGAATGGT; AACCAGCAAGAACAAGAAAAAAGTCTCGTTGTGAGGAAAAAACGCAAAAGCCAGCAGGCT; GGCCCTTCGTATGTGCAGAATTGTGTTAAAGAAAACCAGGGAATATTAGGACTGAGGCAA; CACCTAGGGACACCAAGTGATGAAGATAATGATTCCTCTTTTAGTGATTGTCTTTCTTCT; CCTTCATCTAGTCTGCATTTTGGAGATTCTGATACTGTGACTTCAGATGAGGATAAAGAA; GTCTCTGTAAGACATTCCCAGACCATTTTGAATGCTAAAAGTAGAAGCCATAGTGCACGG; TCTCATAAGTGGCCTCGGACTGAGACAGAATCTGTATCGGGATTGTTAATGAAAAGACCC; TGTTTACATGGCAGTTCGTTACGGAGACTTCCATGCAGAAAGAGATTTGTAAAAAATAAT; TCCTCACAGAGGACACAGAAACAAAAAGAGAGGATATTAATGCAGAGGAAGAAACGAGAA; GTGTTAGCTCGAAGAAAATATGCCTTGCTACCTAGTTCTAGTAGTTCCAGTGAGAATGAC; CTCAGCAGTGAATCCTCTTCTAGCTCATCAACTGAAGGAGAAGAAGATTTGTTTGTTTCT; GCCAGTGAAAACCACCAAAACAATCCAGCTGTTCCCTCAGGAAGTATTGATGAAGATGTT; GTGGTGATAGAAGCTTCCTCCACTCCCCAGGTTACTGCCAATGAAGAAATTAATGTTACC; TCAACTGACAGTGAAGTGGAGATTGTAACAGTTGGAGAAAGCTATCGGTCTCGTTCAACC; CTTGGACACTCCAGATCTCATTGGAGCCAGGGTTCAAGTTCTCATGCAAGTCGGCCACAG; GAGCCACGGAACCGCAGTAGGATTTCTACTGTTATACAGCCCTTGAGGCAGAATGCAGCA; GAAGTTGTGGACCTTACCGTTGATGAAGATGAACCTACTGTAGTACCAACCACTTCTGCA; AGAATGGAATCACAAGCTACTAGCGCTTCCATTAACAATTCAAATCCATCTACCTCTGAG; CAGGCCTCTGATACTGCTTCAGCTGTCACCAGTAGCCAACCTTCCACAGTGTCAGAGACT; TCAGCTACTCTTACAAGCAATAGTACCACTGGCACTTCTATAGGAGATGACTCAAGGAGA; ACTACATCTAGTGCTGTAACGGAAACTGGCCCTCCTGCAATGCCAAGGTTACCTTCCTGC; TGTCCCCAGCACTCACCATGTGGAGGGTCGTCACAGAACCACCATGCATTAGGACATCCT; CATACAAGTTGCTTTCAGCAGCATGGTCACCATTTTCAACATCATCACCACCACCACCAT; ACTCCCCACCCAGCTGTCCCAGTTTCTCCTTCCTTTAGTGATCCTGCTTGCCCTGTGGAA; AGACCTCCACAAGTACAAGCACCTTGTGGAGCAAATAGTAGTTCTGGTACCAGCTATCAT; GAACAGCAGGCATTGCCAGTAGACCTGAGCAACAGTGGTATCAGAAGTCATGGAAGTGGC; AGTTTTCATGGAGCATCTGCATTTGACCCCTGCTGCCCTGTTTCTTCCTCCCGAGCTGCA; ATCTTTGGCCATCAGGCCGCTGCTGCTGCCCCAAGTCAACCTTTATCATCAATAGATGGC; TATGGATCAAGCATGGTTGCGCAGCCCCAGCCCCAGCCCCCTCCACAGCCCTCTCTCTCA; TCATGTCGACATTACATGCCACCCCCTTATGCCTCTTTGACAAGGCCACTTCATCATCAA; GCTTCTGCCTGCCCGCATTCTCATGGAAACCCCCCTCCTCAGACTCAGCCTCCGCCTCAA; GTGGATTATGTTATTCCTCATCCTGTACATGCTTTCCATTCTCAAATATCTTCTCATGCA; ACATCTCATCCTGTGGCACCCCCACCACCAACTCACTTAGCCAGTACAGCTGCACCAATC; CCTCAGCATCTTCCTCCTACACACCAGCCAATTTCGCACCATATTCCAGCCACAGCACCT; CCAGCACAGAGACTGCATCCTCATGAAGTGATGCAGAGGATGGAAGTTCAAAGGAGGAGG; ATGATGCAGCATCCAACGCGGGCACATGAACGCCCCCCACCCCATCCACATAGGATGCAC; CCAAACTATGGTCATGGGCATCATATTCATGTGCCTCAGACTATGTCCTCACATCCTCGA; CAGGCTCCAGAGAGGTCTGCCTGGGAACTGGGAATTGAAGCTGGAGTGACTGCAGCTACT; TATACACCTGGTGCATTGCATCCTCACTTGGCCCATTATCACGCACCTCCTCGACTTCAT; CACTTACAATTAGGAGCTCTTCCTTTAATGGTTCCTGATATGGCAGGCTATCCTCACATC; CGTTACATTTCATCAGGATTGGATGGAACATCATTCAGAGGTCCTTTCAGGGGCAATTTT; GAGGAACTGATTCATTTGGAAGAAAGATTAGGCAATGTCAATCGTGGAGCATCCCAGGGG; ACAATTGAAAGATGTACATATCCACATAAATACAAAAAGAGGAAACTGCACTGCAAACAA; GATGGGGAAGAAGGGACTGAGGAAGACACAGAGGAAAAATGTACTATCTGTTTGTCTATT; TTAGAGGAAGGTGAAGATGTGAGACGTCTTCCATGTATGCACCTTTTCCACCAAGTGTGT; GTTGACCAATGGTTGATTACCAATAAGAAGTGCCCCATATGCAGAGTGGACATTGAGGCC; CAGCTGCCAAGTGAAAGTTGA; ; Clone variation with respect to NM_017610.6; 1056 c=>a;1701 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017610 unedited; TTCGGCACGAGTAGGGGAGCAGCGGCAGTGGCGGCGACGGCGAGGAGGTGTTCGGTTTGC; GCAGCGTAGAAACGCCCGCAGCTTCAGAGAAGGAGTTCTTGTGGGACCAAATTAGGCATT; CTGAACCCACGCCGGCTTAGCTGGGCCGAGTCCAGGCGCTGCCGCACGTACAGTTTTGGT; GGCGGTGACAGACACTGCTCTTGACTCTAGGGGCCTATTAGGCCGACGTCTGAGGCGCAG; ATCGCTGGCTCTCGACGCATTACTCTTTACGCCTTACATTTCTGTCTTCCTTCCTGGGTC; AGTGATTCCCGGACCCTGGAAGAGAAGAGGGTGGCTAATGATTAAGGCTTTCCTTAAAGT; TTCCCATGTCTCAATGGACTCCTGAATATAACGAGCTCTACACCTTAAAAGTGGATATGA; AGAGTGAGATTCCTTCTGATGCACCAAAGACACAGGAGAGTCTGAAAGGGATCCTTTTGC; ATCCAGAGCCCATTGGGGCAGCCAAAAGTTTTCCTGCAGGAGTTGAGATGATTAATAGTA; AAGTGGGGAATGAATTCTCTCACCTGTGTGATGATTCTCAAAAGCAAGAGAAGGAAATGA; ATGGTAACCAGCAAGAACAAGAAAAAAGTCTCGTTGTGAGGAAAAAANCGCAAAGCCAGC; AGGCTGGCCCTTCGTATGTGCAGAATTTGTGTAAAGAAAACCAGGGAATATTANGACTGA; NGGCACACCTANGGACACCAAGTGATGAANGATATGATNTCTCTTTTAGTGATTGTCTTT; CTTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_017610 unedited; CTACCTCGAACTGTCTCCCGAACCAGGTACAGCACTGGGGTAGGGTCACAGGGAATGCCA; CTCCTTTATCTGTTAAGCAAATACAAAGCCGTGGCTTAATGCAAGCTTATTGACATCTTC; ACATATGTATCCATGGAGAACATGTTCCTCCACATACAGACAGTGATATTACTGACTGGA; TGCATTTGCAATCTGGGGCATATTAAGCATATTTAAACAAATAAAATTAGGCTATCCTGT; ATATGAAAGTATCTTGATAATAATATGCATCCTGAAGTAAATTAAAACTTCCTCCAAAGG; AGAAATGTTAGTATGTTAACTTAACTTTATATTCTATATTATGCAGTAAATCTTAACTAT; AGAAATTAAAAATTTTAAATCAATGATCGACTCTTGGAAATGTATGAAGTGGGAGTATAT; AACCATAGCAACAGTAGGTTGACAGTATAATTTGTGCCTCACAGTATCTGCAGATTTACT; GTTTTCAAGTTTAGAGAGAAATAAAACAAGACACATGTAGATTCAGCTGACTTTATTTAC; CTTTAAACTAATAAAAATCCCTTAAAAGTGATCCTGGGACCTTTATAAGTTCTATGAATG; CACAGATACCTGCCAGTGACTTAAATTCCAAATCTTGACTTAATGTGTCTACAAAATCAA; TGTAAGCAGATATCTTAACTTTTCGTGCTTAGCAGAAGACAACAAATTCTACATGTCTTT; TTTCAGTTTCCCCTAAATTTTTTTAAATCTGCAAGTATGTACAATCTACCTAATTGGAAT; TAATATTTAGTGTTCATCAGAGCTGATTCACAGCAACAAAAACTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_017610
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017610.6, NP_060080.6
• RefSeq Size: 5556 bp
• RefSeq ORF: 2961 bp
• Locus ID: 54778
• UniProt ID: Q6ZNA4
• Cytogenetics: 15q22.1-q22.2
• Domains: RING
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]; Transcript Variant: This variant (2) lacks an in-frame segment in the 3' coding region, compared to variant 1, which results in a shorter protein (isoform 2), compared to isoform 1.