M-CSF (CSF1) (NM_000757) Human Untagged Clone

SKU SC128208

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: M-CSF
• Synonyms: CSF-1; MCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC128208 representing NM_000757.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCGGCCGCGAATTCGGCACGAGGGAGGGCTGGCCAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCC; TGGGTCCTCTCGGCGCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCC; CACTCCGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCAGCTG; CCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCTGCTGTTGT; TGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTGTAGCCACATGATTGGGA; GTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGATGGAGACCTCGTGCCAAATTACATTTG; AGTTTGTAGACCAGGAACAGTTGAAAGATCCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAG; ACATAATGGAGGACACCATGCGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGG; AACTCTCTTTGAGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAA; CTTTCTATGAGACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAGAATCTCC; TTGACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAATGCTCCAGCCAAGATG; TGGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATCCCTAGCAGTGACCCGGCCTCTGTCT; CCCCTCATCAGCCCCTCGCCCCCTCCATGGCCCCTGTGGCTGGCTTGACCTGGGAGGACTCTGAGGGAA; CTGAGGGCAGCTCCCTCTTGCCTGGTGAGCAGCCCCTGCACACAGTGGATCCAGGCAGTGCCAAGCAGC; GGCCACCCAGGAGCACCTGCCAGAGCTTTGAGCCGCCAGAGACCCCAGTTGTCAAGGACAGCACCATCG; GTGGCTCACCACAGCCTCGCCCCTCTGTCGGGGCCTTCAACCCCGGGATGGAGGATATTCTTGACTCTG; CAATGGGCACTAATTGGGTCCCAGAAGAAGCCTCTGGAGAGGCCAGTGAGATTCCCGTACCCCAAGGGA; CAGAGCTTTCCCCCTCCAGGCCAGGAGGGGGCAGCATGCAGACAGAGCCCGCCAGACCCAGCAACTTCC; TCTCAGCATCTTCTCCACTCCCTGCATCAGCAAAGGGCCAACAGCCGGCAGATGTAACTGGTACAGCCT; TGCCCAGGGTGGGCCCCGTGAGGCCCACTGGCCAGGACTGGAATCACACCCCCCAGAAGACAGACCATC; CATCTGCCCTGCTCAGAGACCCCCCGGAGCCAGGCTCTCCCAGGATCTCATCACTGCGCCCCCAGGGCC; TCAGCAACCCCTCCACCCTCTCTGCTCAGCCACAGCTTTCCAGAAGCCACTCCTCGGGCAGCGTGCTGC; CCCTTGGGGAGCTGGAGGGCAGGAGGAGCACCAGGGATCGGAGGAGCCCCGCAGAGCCAGAAGGAGGAC; CAGCAAGTGAAGGGGCAGCCAGGCCCCTGCCCCGTTTTAACTCCGTTCCTTTGACTGACACAGGCCATG; AGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTCCAGGAGTCTGTCTTCCACCTGCTGGTGCCCAGTG; TCATCCTGGTCTTGCTGGCCGTCGGAGGCCTCTTGTTCTACAGGTGGAGGCGGCGGAGCCATCAAGAGC; CTCAGAGAGCGGATTCTCCCTTGGAGCAACCAGAGGGCAGCCCCCTGACTCAGGATGACAGACAGGTGG; AACTGCCAGTGTAGAGGGAATTCTAAGCTGGACGCACAGAACAGTCTCTCCGTGGGAGGAGACATTATG; GGGCGTCCACCACCACCCCTCCCTGGCCATCCTCCTGGAATGTGGTCTGCCCTCCACCAGAGCTCCTGC; CTGCCAGGACTGGACCAGAGCAGCCAGGCTGGGGCCCCTCTGTCTCAACCCGCAGACCCTTGACTGAAT; GAGAGAGGCCAGAGGATGCTCCCCATGCTGCCACTATTTATTGTGAGCCCTGGAGGCTCCCATGTGCTT; GAGGAAGGCTGGTGAGCCCGGCTCAGGACCCTCTTCCCTCAGGGGCTGCACCCTCCTCTCACTCCCTTC; CATGCCGGAACCCAGGCCAGGGACCCACCGGCCTGTGGTTTGTGGGAAAGCAGGGTGGACGCTGAGGAG; TGAAAGAACCCTGCACCCAGAGGGCCTGCCTGGTGCCAAGGTATCCCAGCCTGGACAGGCATGGACCTG; TCTCCAGAGAGAGGAGCCTGAAGTTCGTGGGGCGGGACAGCGTCGGCCTGATTTCCCGTAAAGGTGTGC; AGCCTGAGAGACGGGAAGAGGAGGCCTCTGGACCTGCTGGTCTGCACTGACAGCCTGAAGGGTCTACAC; CCTCGGCTCACCTAAGTGCCCTGTGCTGGTTGCCAGGCGCAGAGGGGAGGCCAGCCCTGCCCTCAGGAC; CTGCCTGACCTGCCAGTGATGCCAAGAGGGGGATCAAGCACTGGCCTCTGCCCCTCCTCCTTCCAGCAC; CTGCCAGAGCTTCTCCAGGAGGCCAAGCAGAGGCTCCCCTCATGAAGGAAGCCATTGCACTGTGAACAC; TGTACCTGCCTGCTGAACAGCCTGCCCCCGTCCATCCATGAGCCAGCATCCGTCCGTCCTCCACTCTCC; AGCCTCTCCCCATGGCCTCCATGACTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000757 unedited; GTTCGGATTTGTATACGACTTATATAGGGCGGCCGCGAATTCGCACGAGGGAGGGCTGGC; CAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGCGCCAGAGCCG; CTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTCCGCAGCAGCC; AGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCAGCTGCCCGTA; TGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGCTCCCTGCTGT; TGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAGTACTGTAGCC; ACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGTCAGATGGAGA; CCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGATCCAGTGTGCT; ACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGGACACCATGCGCTTCAGA; GATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTGAGGCTGGAA; GAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACTTCCTATGG; AGACACCTCTCCATTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAGAATCTCC; TTGACAGGGACTGGGAATATTTTCAGCAAGAACTGCAACAACAGCCTTGCTGAGTGCTCC; ACCCAGAAGTGGTGACCAAGCCTGATGGCAATGCCTGTGCCCCAAAACCATCCCTAGCAG; TGACCCGGCCTCTGGGTTCCCTAATAAGCCCCTTGCCCCCTCCTGGGGCCTGGGGGCTGG; GCTGACCGGGGAGGACTCTGAGGGAACG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000757 unedited; AGAGCTGGNATAGTGGAGACGGTCGGTTGCTGGCTCATGGATGGACCGGGGCAGGCTGTT; CAGCAAGCAGGTACAGTGTTCACAGTGCAATGGCTTCCTTCATGAGGGGAGCCTCTGCTT; GGCCTCCTGGAGAAGCTCTGGCAGGTGCTGGAAGGAGGAGGGGCAGAGGCCTGTGCTTGA; TCCCCCTCTTGGCATCACTGGCAGGTCAGGCAGGTCCTGAGGGCAGGGCTGGCCTCCCCT; CTGCGCCTGGCAACCAGCACAGGGCACTTATGTGAGCCGAGGGTGTAGACCCTTCAGGCT; GTCAGTGCAGACCAGCAGGTCCAGAGGCCTCCTCTTCCCGTCTCTCAGGCTGCACACCTT; TACGGGAAATCAGGCCGACGCTGTCCCGCCCCACGAACTTCAGGCTCCTCTCTCTGGAGA; CAGGTCCATGCCTGTCCAGGCTGGGATACCTTGGCACCAGGCAGGCCCTCTGGGTGCAGG; GTTCTTTCACTCCTCAGCGTCCACCCTGCTTTCCCACAAACCACAAGCCGGTGGGTCCCT; GGGCCTGGGTTCCGGCATGGAAGGGAGTGAGAGGAGGGTGCAGCCCCTGAGGGAAGAGGG; TCCTGAGCCGGGGTCACCAACCTTTCTCAAGCACATGGGGAGCCTCCCAGGGCTCACAAT; AAATAGTGGCACCCCTGGGGAGCATCCCTCTGGGCTGTTTTCTTTCATTCAAAGGGTCTG; CCGGGTTGGGACAAAAGGGCCCCCAGCCCGGCCTGCCCTGGTCCCAATCCCTGGAAGCCA; GTACCTCTGGGTGAGGGGCAAACCACATTCTCAGAGA;
• Restriction Sites: SgfI-MluI
• ACCN: NM_000757
• Insert Size: 2866 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000757.3
• RefSeq Size: 2749 bp
• RefSeq ORF: 2866 bp
• Locus ID: 1435
• UniProt ID: P09603
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
• MW: 103.1 kDa
• Summary: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses a downstream in-frame AUG start codon, which is supported by homology and publication data. The use of the 5'-most in-frame AUG in the transcript would result in a 74 aa N-terminal extension, but this N-terminal extension is not conserved in other species, and it would eliminate an N-terminal signal peptide, which is known to be present based on experimental evidence (PMIDs 8262907, 3498652).