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PTGER3 (NM_000957) Human Untagged Clone

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SKU
SC128068
PTGER3 (untagged)-Human prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 1
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PTGER3
Synonyms EP3; EP3-I; EP3-II; EP3-III; EP3-IV; EP3e; MGC27302; MGC141828; MGC141829
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_000957, the custom clone sequence may differ by one or more nucleotides


ATGAAGGAGACCCGGGGCTACGGAGGGGATGCCCCCTTCTGCACCCGCCTCAACCACTCCTACACAGGCA
TGTGGGCGCCCGAGCGTTCCGCCGAGGCGCGGGGCAACCTCACGCGCCCTCCAGGGTCTGGCGAGGATTG
CGGATCGGTGTCCGTGGCCTTCCCGATCACCATGCTGCTCACTGGTTTCGTGGGCAACGCACTGGCCATG
CTGCTCGTGTCGCGCAGCTACCGGCGCCGGGAGAGCAAGCGCAAGAAGTCCTTCCTGCTGTGCATCGGCT
GGCTGGCGCTCACCGACCTGGTCGGGCAGCTTCTCACCACCCCGGTCGTCATCGTCGTGTACCTGTCCAA
GCAGCGTTGGGAGCACATCGACCCGTCGGGGCGGCTCTGCACCTTTTTCGGGCTGACCATGACTGTTTTC
GGGCTCTCCTCGTTGTTCATCGCCAGCGCCATGGCCGTCGAGCGGGCGCTGGCCATCAGGGCGCCGCACT
GGTATGCGAGCCACATGAAGACGCGTGCCACCCGCGCTGTGCTGCTCGGCGTGTGGCTGGCCGTGCTCGC
CTTCGCCCTGCTGCCGGTGCTGGGCGTGGGCCAGTACACCGTCCAGTGGCCCGGGACGTGGTGCTTCATC
AGCACCGGGCGAGGGGGCAACGGGACTAGCTCTTCGCATAACTGGGGCAACCTTTTCTTCGCCTCTGCCT
TTGCCTTCCTGGGGCTCTTGGCGCTGACAGTCACCTTTTCCTGCAACCTGGCCACCATTAAGGCCCTGGT
GTCCCGCTGCCGGGCCAAGGCCACGGCATCTCAGTCCAGTGCCCAGTGGGGCCGCATCACGACCGAGACG
GCCATTCAGCTTATGGGGATCATGTGCGTGCTGTCGGTCTGCTGGTCTCCGCTCCTGATAATGATGTTGA
AAATGATCTTCAATCAGACATCAGTTGAGCACTGCAAGACACACACGGAGAAGCAGAAAGAATGCAACTT
CTTCTTAATAGCTGTTCGCCTGGCTTCACTGAACCAGATCTTGGATCCTTGGGTTTACCTGCTGTTAAGA
AAGATCCTTCTTCGAAAGTTTTGCCAGATGAGAAAAAGAAGACTCAGAGAGCAAGCTCCTCTTCTTCCCA
CCTCTACTGTGATTGATCCTTCAAGGTTCTGTGCTCAGCCCTTCCGTTGGTTCTTGGATTTGTCCTTTCC
CGCCATGTCTTCATCACATCCACAACTTCCACTAACACTTGCGAGCTTCAAACTTCTTAGAGAACCCTGC
AGTGTCCAGCTAAGCTGA

3' Read Nucleotide Sequence
>OriGene 3' read for NM_000957 unedited
ACTTTGGNGATGGCAACTTCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGGATGCC
ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAAGTCATCAGCTT
AGCTGGACACTGCAGGGTTCTCTAAGAAGTTTGAAGCTCGCAAGTGTTAGTGGAAGTTGT
GGATGTGATGAAGACATGGCGGGAAAGGACAAATCCAAGAACCAACGGAAGGGCTGAGCA
CAGAACCTTGAAGGATCAATCACAGTAGAGGTGGGAAGAAGAGGAGCTTGCTCTCTGAGT
CTTCTTTTTCTCATCTGGCAAAACTTTCGAAGAAGGATCTTTCTTAACAGCAGGTAAACC
CAAGGATCCAAGATCTGGTTCAGTGAAGCCAGGCGAACAGCTATTAAGAAGAAGTTGCAT
TCTTTCTGCTTCTCCGTGTGTGTCTTGCAGTGCTCAACTGATGTCTGATTGAAGATCATT
TTCAACATCATTATCAGGAGCGGAGACCAGCAGACCGACAGCACGCACATGATCCCCATA
AGCTGAATGGCCGTCTCGGTCGTGATGCGGCCCCACTGGGCACTGGACTGAGATGCCGTG
GCCTTGGCCCGGCAGCGGGACACCAGGGCCTTAATGGTGGCCAGGTTGCAGGAAAAGGTG
ACTGTCAGCGCCAAGAGCCCCAGGAAGGCAAAGGCAGAGGCGAAGAANAGGTGCCCCAGT
TATGCGAAGAGCTAGTCCCGTTGCCCCCTCGCCCGGTGCTGATGAAGCACCACGTCCCGG
GCCACTGGACGGTGTACTGGCCCACGCCCAGCACCGGCAGCAGGGGCGAAGCGAGCACGG
CCAGCCACACGCCGAGCAGCACAGCGCGGGT
Restriction Sites Please inquire |
ACCN NM_000957
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000957.2, NP_000948.2
RefSeq Size 2082 bp
RefSeq ORF 1278 bp
Locus ID 5733
Cytogenetics 1p31.1
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transcription Factors, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
Summary The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Aug 2009
Transcript Variant: This variant (1) encodes the longest isoform (1). Another name for this transcript is EP3f.
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Documents
Resources
"NM_000957" in other vectors (4)
SKU Description Size Price
RC222393 PTGER3 (Myc-DDK-tagged)-Human prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 1 10 ug
$686.00
RC222393L3 Lenti-ORF clone of PTGER3 (Myc-DDK-tagged)-Human prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 1 10 ug
$986.00
RC222393L4 Lenti-ORF clone of PTGER3 (mGFP-tagged)-Human prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 1 10 ug
$986.00
RG222393 PTGER3 (tGFP-tagged) - Human prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 1 10 ug
$886.00

file-alt Citations

Shear-induced interleukin-6 synthesis in chondrocytes: roles of E prostanoid (EP) 2 and EP3 in cAMP/protein kinase A- and PI3-K/Akt-dependent NF-kappaB activation
Wang, Zhu, Lee et al
J Biol Chem (2010) 285 (32), 24793-804 DOI: 10.1074/jbc.M110.110320
AI Summary: OriGene products contributed to this paper by providing EP2 shRNA (OriGene) for selective knockdown of the EP2 receptor, enabling the authors to investigate its role in shear-induced interleukin-6 synthesis in chondrocytes.
Showing 1-1 of 1 papers.
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