PHC1 (NM_004426) Human Untagged Clone

SKU SC127500

PHC1 (untagged)-Human polyhomeotic homolog 1 (Drosophila) (PHC1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PHC1
• Synonyms: EDR1; HPH1; MCPH11; RAE28
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127500 sequence for NM_004426 edited (data generated by NextGen Sequencing); ATGGAGACTGAGAGCGAGCAGAACTCCAATTCCACCAATGGGAGTTCTAGCTCAGGGGGC; AGCTCTCGGCCCCAGATAGCTCAAATGTCACTATATGAACGACAAGCAGTGCAGGCTCTG; CAAGCACTGCAGCGGCAGCCCAATGCAGCTCAGTATTTCCACCAGTTCATGCTCCAGCAG; CAGCTCAGTAATGCCCAGCTGCATAGCCTGGCTGCCGTCCAGCAGGCCACAATTGCTGCC; AGTCGGCAGGCCAGCTCCCCAAACACCAGCACTACACAGCAGCAGACTACCACCACCCAG; GCCTCGATCAATCTGGCCACCACATCGGCCGCCCAGCTCATCAGCCGATCCCAGAGTGTG; AGCTCTCCCAGTGCTACCACCTTGACCCAATCTGTGCTACTGGGGAACACCACCTCCCCA; CCCCTCAACCAGTCTCAGGCCCAGATGTATCTACGGCCACAGCTGGGAAACCTATTGCAG; GTAAACCGAACCCTGGGTCGGAATGTGCCTCTAGCCTCCCAACTCATCCTGATGCCTAAT; GGGGCGGTGGCTGCAGTCCAGCAGGAGGTGCCATCTGCTCAGTCTCCTGGAGTTCATGCA; GATGCAGATCAGGTTCAGAACTTGGCAGTAAGGAATCAACAGGCCTCAGCTCAAGGACCT; CAGATGCAAGGCTCCACTCAGAAGGCCATTCCTCCAGGAGCCTCCCCTGTCTCTAGCCTC; TCCCAGGCCTCTAGCCAGGCCCTAGCGGTGGCACAGGCTTCCTCTGGGGCCACAAACCAG; TCCCTCAACCTTAGTCAAGCTGGTGGAGGCAGTGGGAATAGCATCCCAGGGTCCATGGGT; CCAGGTGGAGGTGGGCAGGCACATGGTGGTTTGGGTCAGTTGCCTTCCTCAGGAATGGGT; GGTGGGAGCTGTCCCAGGAAGGGTACAGGAGTGGTGCAGCCCTTGCCTGCAGCCCAAACA; GTGACTGTGAGCCAGGGCAGCCAGACAGAGGCAGAAAGTGCAGCAGCCAAGAAGGCAGAA; GCAGATGGGAGTGGCCAGCAGAATGTGGGCATGAACCTGACACGGACAGCCACACCTGCG; CCCAGCCAGACACTTATTAGCTCAGCCACCTACACACAGATCCAGCCCCATTCACTGATT; CAGCAACAGCAACAGATCCACCTCCAGCAGAAACAGGTGGTGATCCAGCAGCAGATTGCC; ATCCACCACCAGCAGCAGTTCCAGCACCGGCAGTCCCAGCTCCTTCACACAGCTACACAC; CTCCAGTTGGCGCAGCAGCAGCAGCAGCAACAACAGCAACAGCAGCAACAGCAGCAGCCG; CAAGCCACCACCCTCACTGCCCCTCAGCCACCACAGGTCCCACCTACTCAGCAGGTCCCA; CCTTCCCAGTCCCAGCAGCAAGCCCAAACCCTGGTCGTTCAGCCCATGCTTCAGTCTTCA; CCCTTGTCTCTTCCACCTGATGCAGCCCCTAAGCCACCAATTCCCATCCAATCCAAACCA; CCTGTAGCACCTATCAAGCCGCCTCAGTTAGGGGCCGCTAAGATGTCAGCTGCCCAGCAA; CCACCACCCCATATCCCTGTGCAAGTTGTAGGCACTCGACAGCCAGGTACAGCCCAGGCA; CAGGCTTTGGGGTTGGCACAGCTGGCAGCTGCTGTACCTACTTCCCGGGGGATGCCAGGT; ACAGTGCAGTCTGGTCAGGCCCATTTGGCCTCCTCGCCACCTTCATCCCAGGCTCCTGGT; GCACTGCAGGAGTGCCCTCCCACATTGGCCCCTGGGATGACCCTTGCTCCTGTGCAGGGG; ACAGCACATGTGGTAAAGGGTGGGGCTACCACCTCCTCACCTGTTGTAGCCCAGGTCCCT; GCTGCCTTCTATATGCAGTCTGTGCACTTGCCGGGTAAACCCCAGACATTGGCTGTCAAA; CGCAAGGCTGACTCTGAGGAGGAGAGAGATGATGTCTCCACATTGGGTTCAATGCTTCCT; GCCAAGGCATCTCCAGTAGCAGAAAGCCCAAAAGTCATGGACGAGAAGAGCAGTCTTGGA; GAAAAAGCTGAATCAGTGGCTAATGTGAATGCTAATACTCCAAGCAGTGAACTAGTAGCC; TTGACCCCCGCCCCTTCAGTACCGCCTCCTACACTAGCCATGGTGTCTAGACAAATGGGT; GACTCAAAACCCCCACAGGCCATCGTGAAGCCCCAGATTCTCACCCACATCATTGAAGGC; TTTGTTATCCAGGAAGGAGCAGAACCTTTCCCGGTGGGTTGTTCTCAGTTACTGAAGGAG; TCTGAGAAGCCACTACAGACTGGCCTTCCGACAGGGCTGACTGAGAATCAGTCAGGTGGC; CCTTTGGGAGTGGACAGCCCATCTGCTGAGTTAGATAAGAAGGCGAATCTCCTGAAGTGC; GAGTACTGTGGGAAGTACGCCCCCGCAGAGCAGTTTCGTGGCTCTAAGAGGTTCTGCTCC; ATGACTTGCGCTAAGAGGTACAATGTGAGCTGTAGCCATCAGTTCCGGCTGAAGAGGAAA; AAAATGAAAGAGTTTCAAGAAGCCAACTATGCTCGCGTTCGCAGGCGTGGACCCCGCCGC; AGCTCCTCTGACATTGCCCGTGCCAAGATTCAGGGCAAGTGCCACCGGGGTCAAGAAGAC; TCTAGCCGGGGTTCAGATAATTCCAGTTATGATGAAGCACTCTCTCCAACATCTCCTGGG; CCTTTATCAGTAAGAGCTGGGCATGGAGAACGTGACCTGGGGAATCCCAATACAGCTCCA; CCTACACCGGAATTACATGGCATCAACCCTGTGTTCCTGTCCAGTAATCCCAGCCGTTGG; AGTGTAGAGGAGGTGTACGAGTTTATTGCTTCTCTCCAAGGCTGCCAAGAGATTGCAGAG; GAATTTCGCTCACAGGAGATTGATGGACAGGCCCTTTTATTACTTAAAGAAGAACATCTT; ATGAGTGCCATGAACATCAAGCTGGGCCCTGCCCTCAAGATCTGCGCCAAGATAAATGTC; CTCAAGGAGACCTAA; ; Clone variation with respect to NM_004426.2; 93 t=>a;918 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004426 unedited; TATAGGCGGCCGCGAATTCGGCACGAGGCTCGTGTCTCTGAGTCCAGTGAACTAAATCCA; GTAATCTATAAAACTGTGCCGTGCTGCCTCTAATCCTTACCCACTTTTTTTCTTCACATG; CTGATAATGAGGATTCATGGGTCTTGAGTCAGACAGAGCACCAGCCTTGGGGACCCTGGA; CCACTATCATGGAGACTGAGAGCGAGCAGAACTCCAATTCCACCAATGGGAGTTCTAGCT; CAAGGGGCAGCTCTCGGCCCCAGATAGCTCAAATGTCACTATATGAACGACAAGCAGTGC; AGGCTCTGCAAGCACTGCAGCGGCAGCCCAATGCAGCTCAGTATTTCCACCAGTTCATGC; TCCAGCAGCAGCTCAGTAATGCCCAGCTGCATAGCCTGGCTGCCGTCCAGCAGGCCACAA; TTGCTGCCAGTCGGCAGGCCAGCTCCCCAAACACCAGCACTACACAGCAGCAGACTACCA; CCACCCAGGCCTCGATCAATCTGGCCACCACATCGGCCGCCCAGCTCATCAGCCGATCCC; AGAGTGTGAGCTCTCCCAGTGCTACCACCTTGACCCAATCTGTGCTACTGGGGAACACCA; CCTCCCCACCCCTCAACCAGTCTCAGGCCCAGATGTATCTACGGCCACAGCTGGGANACC; TATTGCAGGTAAACCGAACCCTGGGTCGGAATGTGCCTCTAGCCTNCCAACTCATNCTGA; TGCCTATGGGGGCGGTGGCTGCAGTCCAGCAGGAGGTGCCATCTGCTCAGTCTCCTGGAG; TCATGCAATGCAGNACAGGTTCAGAACTTGCAGTAGGGAATCACAGGCCTCAGCTCAGGG; ACCTCANATGCAGGCTCACTCAAAAGGCATTNNCTCAGAGCTNCCTGCTTGAANNCCTTC; CAGGCTNTAGCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004426 unedited; NNTTACTTGNCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTACTGA; GGAAAAAAAATTTAATATTTTTGTTATATAAGGAATAGTGCCTAAGGCAGGTACTTATAC; TCCTGACCTCAGCCCCACACACTCTGGTTTTATAAAGCTATAGGACAGAGCAGAGATGGA; ACTGAAAAACAGGGTAGAAAATAACATAAATTGGAGGGGAACAGTGGGATGCAGAAAGAA; TGACAACAGCCACATGTGCCCCAGTCAAATACTTTTAGTCCCTGCAGCAGAAGATGCCAA; CCAAGTCTCTATACTGGCTGGGGATCCTGCCATGGATGCAGGAGAAAAAATTCAATCACA; AGGGAGTAAGTGACAACAAAAAGAAAAAACTCTGAGCCACAGCTACACTGGGAGGGGGGA; GGACCAGATTTTGCTACTTCTGTCACACAAGCTAGAAAAACCCTACCTCTCCCATTGGGC; AGGCAACCTGACATTCCACCCAGTTCCTTGCCTTACCATACTATGTATTCCTCCTCTTGG; AGAATTAAGACCTGGGCTAAATGCTCCACTCCAGGATAAGGCTGAGCAGTCCTAGTTCTC; TAGACTGCCTCTTTTATAAAGATTGGATTAAGGTACGTGAAATGGTGAGAACTGTAAAGA; TTTTAAAAAATAAAGTAGAACCCAGAGAAAATGTCAAAGCTGCCGCCATGTAGCACCAGC; AACCAATTCTTGCACTTCTCTTCCCTGTCTCAGTAATCCCCTACAGAAGGTTACATGATT; GGGACAACTCTTNTCTTCCCTGCAAGTCTGCTGGTACCCAGTTATACCTGGACAGGGGAN; AGTGTCTGCCTTAGCTGGTNTGTGCAAGAGGCCCACCCTAGTCTNNCTGAGACATTNNAT; CTGGCGCAGATCTTGAGGCAGGNCCCAGCTGATGTCATGGACTCATAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004426
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004426.2, NP_004417.2
• RefSeq Size: 5206 bp
• RefSeq ORF: 3015 bp
• Locus ID: 1911
• UniProt ID: P78364
• Cytogenetics: 12p13.31
• Domains: SAM
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency
• Summary: This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq, Jul 2008]