TMTC2 (NM_152588) Human Untagged Clone

SKU SC127237

TMTC2 (untagged)-Human transmembrane and tetratricopeptide repeat containing 2 (TMTC2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TMTC2
• Synonyms: IBDBP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127237 sequence for NM_152588 edited (data generated by NextGen Sequencing); ATGATTGCAGAGTTGGTGAGCAGCGCTCTGGGGCTCGCCTTGTATCTCAACACCCTGAGT; GCGGATTTCTGCTATGATGACAGCCGTGCTATCAAGACTAATCAGGACCTTCTCCCAGAA; ACTCCATGGACGCACATTTTCTACAATGATTTTTGGGGGACTCTTCTAACCCACAGTGGC; AGCCACAAGTCCTACCGGCCACTCTGCACTCTTTCTTTTCGCCTGAACCATGCCATTGGA; GGGTTGAATCCCTGGAGCTACCATCTTGTCAATGTCCTGTTGCATGCAGCAGTCACTGGT; CTCTTCACAAGCTTCTCCAAGATCCTCCTTGGTGATGGATACTGGACATTCATGGCTGGC; TTGATGTTTGCTTCTCACCCCATTCACACGGAGGCAGTGGCAGGAATCGTGGGACGAGCC; GATGTCGGGGCCAGTCTCTTCTTTCTCCTCTCCTTGCTCTGCTACATTAAACACTGTTCT; ACAAGAGGCTACTCAGCCAGAACCTGGGGCTGGTTCCTGGGGTCAGGACTGTGCGCAGGA; TGCAGCATGTTGTGGAAGGAACAAGGAGTGACTGTTCTCGCAGTTTCAGCAGTTTATGAT; GTCTTTGTCTTTCACAGGCTGAAAATAAAACAGATATTACCTACCATTTACAAAAGGAAG; AACTTGTCGCTTTTCCTAAGCATTAGTTTGTTAATTTTCTGGGGTTCCTCCCTTTTGGGT; GCCCGGTTATACTGGATGGGAAACAAACCACCAAGCTTTTCCAACTCGGACAACCCCGCT; GCTGATTCGGACAGCCTCCTCACCCGCACTCTCACCTTCTTCTACTTGCCAACCAAGAAC; CTCTGGCTGTTGCTATGTCCAGATACCCTCAGTTTTGATTGGTCAATGGATGCTGTGCCT; CTGCTCAAAACAGTTTGTGACTGGAGAAACCTACACACTGTGGCCTTCTATACTGGACTC; CTTCTCCTTGCCTACTATGGTTTGAAGAGCCCGAGCGTAGACAGAGAATGCAATGGGAAA; ACTGTAACAAATGGCAAGCAGAATGCAAATGGACATAGCTGCCTTTCAGATGTGGAGTAC; CAGAACTCAGAGACTAAGTCCAGCTTTGCATCCAAAGTAGAAAATGGCATTAAAAACGAT; GTATCACAGAGAACCCAGCTTCCTTCTACGGAGAACATTGTTGTTCTGTCTTTATCTTTG; TTAATCATACCCTTTGTTCCTGCCACGAACCTGTTTTTCTATGTCGGCTTTGTAATTGCA; GAGCGAGTATTATATATTCCTAGTATGGGCTTCTGCCTACTGATTACAGTGGGTGCTAGA; GCCCTTTATGTCAAAGTCCAAAAGCGGTTCCTCAAGAGCTTGATTTTTTATGCTACAGCT; ACACTAATTGTTTTTTATGGACTCAAGACTGCGATCAGGAATGGAGACTGGCAGAATGAG; GAAATGCTTTATAGATCAGGGATAAAAGTAAACCCAGCTAAAGCATGGGGTAACCTTGGA; AATGTTCTGAAGAGTCAGAGCAAAATTTCTGAAGCTGAAAGCGCCTATAGAAATGCTTTG; TACTACCGCAGCAACATGGCTGACATGCTTTATAATTTAGGGCTACTTCTCCAGGAGAAC; AGCAGGTTTGCAGAAGCACTACATTATTATAAATTGGCCATTGGGAGCAGGCCTACCCTG; GCTTCTGCATATTTAAATACCGGTATTATTCTAATGAACCAAGGAAGGACGGAAGAAGCC; CGACGGACATTCTTAAAGTGTTCGGAGATCCCAGATGAAAACCTAAAGGACCCTCATGCA; CACAAGAGCTCTGTTACCAGTTGTTTGTACAACCTAGGAAAGCTGTATCATGAGCAGGGA; CACTATGAGGAAGCCCTTAGTGTATACAAGGAAGCAATTCAGAAAATGCCAAGGCAGTTT; GCCCCACAGAGCTTGTACAACATGATGGGTGAAGCATATATGCGTTTAAGCAAACTCCCC; GAAGCAGAGCATTGGTATATGGAATCACTGAGATCCAAGACTGACCACATCCCTGCTCAT; CTCACCTATGGGAAGCTGCTAGCTCTAACAGGTCGTAAGAGTGAGGCTGAAAAGCTCTTC; TTGAAGGCTATTGAGCTGGATCCCACCAAAGGAAACTGTTACATGCATTATGGTCAGTTT; CTTCTGGAAGAAGCTCGTCTCATAGAAGCAGCTGAGATGGCAAAAAAAGCAGCTGAACTA; GACAGCACAGAGTTTGATGTTGTCTTCAATGCTGCCCACATGCTCAGACAGGCTAGCCTC; AATGAAGCAGCTGAGAAGTATTATGATCTGGCAGCCAGGCTGAGGCCTAATTATCCGGCT; GCTTTGATGAACCTGGGAGCCATTCTGCACCTCAATGGCAGACTCCAGAAGGCCGAGGCC; AACTACCTGCGGGCCCTGCAGCTCAAGCCAGACGATGTCATCACACAGTCCAATCTCCGC; AAACTGTGGAACATCATGGAAAAACAAGGCTTAAAGACTTCTAAGACCTGA; ; Clone variation with respect to NM_152588.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152588 unedited; ACGAGGCTGGAGTCTCCAGCCACCGCTTCTCACTTCACTGGAGAAGGGAGCTGGGGCTGG; GGCTGGGGCTGGGGCTGGGAGGGAGCTAATAAACATTGAATGTGCAGCAGCTGCCTTGGC; GGCCGGAGTCCGCCCGAGCGACACCGGAGCAGTGCGCCCGGGAGGCGGCGAGGGGAGGCG; GTTCGCCTTGTCCCTCCCACCCGCGCCTCTTTCTCTCTCTTCCTCCAGCTGGTCCCAGAG; CCCGGCTTGGTCCGGTCCCTCTGCCCCCATCCCGCACCCTTCCACCTCCTCCGAGTCCCA; CTCCTCACCTAGGACGCCCCAAACTGCCATGGGTTAGGGTGGGGATCGCGACCCGCGCGA; AAGACCAGCCCTGCGCTTCCGCCGGGGGACGCGGAGCCCAAACGCCGCTCACCGCTTGCG; GGCGCCGGGCATGGGGAGTGTGGTGTGAGCCCGCACCCGGGGAGGACGCAGGAGCTGCGG; AGACGGGCGCGAGGAGGAGGAGAGGAGTCGTGGATTGGAAGGACCCGAGGGAGGGAGGGT; GGGGAAGCGAGGGAAAAGTGAAGCTGGGAGGAGAAGGCGGCGGAAGGTGGAGATTGATGC; TTCTGTTTTTTGTTGCCGCTGCTGCCCTCGCGCTGGGAGCCGAGCCGGAGGGAANGCGGT; GGAGAGATGATTGCAGAGTTGGTGAGCAGCGCTCTGGGGCTCGCCTTGTATCTCAACACC; CTGAGTGCGGATTTCTGCTATGATGACAGCCGNGCTATCAAGACTAATCANGANCTTTTT; CCAAAAACTNCATGGGACGCNCATTTTTCTACAATGATTTTTGGGGGACTCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152588 unedited; GGCACTGGGGAAGGGAGTCACAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATG; ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAATTGCCAATGCACT; AAAAGATGAATAATACACTTCAACCCACTATTTTGGTTTGACCACGGGTTTGTTGTTAAA; TATCATTCTAGAAAACACAGTGATTTAGTTATAATTTAACTTTTGGCCCTTCAGTGAAAT; GTTTACAAAATAATATATATATTACAGAAGGGCTTTCTTATTAATGCCATGAAAAAACCT; TTCTCACTACATTTACCCTTCTCACAAAAACACTAATGCCATAGGAACACTACTTTCAAT; TATTTATCCCACTGTATTCAGATACATAGAAAAACATAAAATATGGAGAAAACATAATAT; GATGTATTTGCATGTCCAGCTTCTTTTTAATGGAGAGGCTGATCATATATACATGGAGTG; TGAAAGGGAAGGAAAAACAGGTAATTTTCAGTTGCAAAGAACTGGCTTAATGCATCAAGT; TTTGTTATTCTGCTATNGTAGAATAATAAATAAATAAATATGTAGGGTATAGTATAGCTA; CTTTCTCTGTGAAGAAGTTGATAACGGACTAGTGTTTTATGAAGTTAATTTTTAGTGGCA; AAGTTTGATTGCAAGCCAAAAATGTTCAAAAAAAATGGTGTTTCTCTACCCAATTTAAAG; TCCTTTTTCTGTGAAAAGATCATTGAAATATTTACCAACAGGGTAAGGAAATAAAAACCC; ATTTTTGCAGTGAAAAAAAGTTTTCAACTGAAAAAACCCTTGGCAAAAAAACTAAAAACC; TGGCGGCAAAGGGCCCACCTCTATGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_152588
• Insert Size: 5200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152588.1, NP_689801.1
• RefSeq Size: 4855 bp
• RefSeq ORF: 2511 bp
• Locus ID: 160335
• UniProt ID: Q8N394
• Cytogenetics: 12q21.31
• Domains: TPR
• Protein Families: Transmembrane
• Summary: The protein encoded by this gene is an integral membrane protein localized to the endoplasmic reticulum (ER). The encoded protein contains many tetratricopeptide repeats, sequences known for being involved in protein-protein interactions. This protein binds both the calcium uptake pump SERCA2B and the carbohydrate-binding chaperone calnexin, and it appears to play a role in calcium homeostasis in the ER. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).