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SEMA4B (NM_020210) Human Untagged Clone

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SKU
SC127234
SEMA4B (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (SEMA4B), transcript variant 1
  $767.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SEMA4B
Synonyms SEMAC; SemC
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_020210, the custom clone sequence may differ by one or more nucleotides


ATGCTGCGCACCGCGATGGGCCTGAGGAGCTGGCTCGCCGCCCCATGGGGCGCGCTGCCGCCTCGGCCAC
CGCTGCTGCTGCTCCTGCTGCTGCTGCTCCTGCTGCAGCCGCCGCCTCCGACCTGGGCGCTCAGCCCCCG
GATCAGCCTGCCTCTGGGCTCTGAAGAGCGGCCATTCCTCAGATTCGAAGCTGAACACATCTCCAACTAC
ACAGCCCTTCTGCTGAGCAGGGATGGCAGGACCCTGTACGTGGGTGCTCGAGAGGCCCTCTTTGCACTCA
GTAGCAACCTCAGCTTCCTGCCAGGCGGGGAGTACCAGGAGCTGCTTTGGGGTGCAGACGCAGAGAAGAA
ACAGCAGTGCAGCTTCAAGGGCAAGGACCCACAGCGCGACTGTCAAAACTACATCAAGATCCTCCTGCCG
CTCAGCGGCAGTCACCTGTTCACCTGTGGCACAGCAGCCTTCAGCCCCATGTGTACCTACATCAACATGG
AGAACTTCACCCTGGCAAGGGACGAGAAGGGGAATGTCCTCCTGGAAGATGGCAAGGGCCGTTGTCCCTT
CGACCCGAATTTCAAGTCCACTGCCCTGGTGGTTGATGGCGAGCTCTACACTGGAACAGTCAGCAGCTTC
CAAGGGAATGACCCGGCCATCTCGCGGAGCCAAAGCCTTCGCCCCACCAAGACCGAGAGCTCCCTCAACT
GGCTGCAAGACCCAGCTTTTGTGGCCTCAGCCTACATTCCTGAGAGCCTGGGCAGCTTGCAAGGCGATGA
TGACAAGATCTACTTTTTCTTCAGCGAGACTGGCCAGGAATTTGAGTTCTTTGAGAACACCATTGTGTCC
CGCATTGCCCGCATCTGCAAGGGCGATGAGGGTGGAGAGCGGGTGCTACAGCAGCGCTGGACCTCCTTCC
TCAAGGCCCAGCTGCTGTGCTCACGGCCCGACGATGGCTTCCCCTTCAACGTGCTGCAGGATGTCTTCAC
GCTGAGCCCCAGCCCCCAGGACTGGCGTGACACCCTTTTCTATGGGGTCTTCACTTCCCAGTGGCACAGG
GGAACTACAGAAGGCTCTGCCGTCTGTGTCTTCACAATGAAGGATGTGCAGAGAGTCTTCAGCGGCCTCT
ACAAGGAGGTGAACCGTGAGACACAGCAGTGGTACACCGTGACCCACCCGGTGCCCACACCCCGGCCTGG
AGCGTGCATCACCAACAGTGCCCGGGAAAGGAAGATCAACTCATCCCTGCAGCTCCCAGACCGCGTGCTG
AACTTCCTCAAGGACCACTTCCTGATGGACGGGCAGGTCCGAAGCCGCATGCTGCTGCTGCAGCCCCAGG
CTCGCTACCAGCGCGTGGCTGTACACCGCGTCCCTGGCCTGCACCACACCTACGATGTCCTCTTCCTGGG
CACTGGTGACGGCCGGCTCCACAAGGCAGTGAGCGTGGGCCCCCGGGTGCACATCATTGAGGAGCTGCAG
ATCTTCTCATCGGGACAGCCCGTGCAGAATCTGCTCCTGGACACCCACAGGGGGCTGCTGTATGCGGCCT
CACACTCGGGCGTAGTCCAGGTGCCCATGGCCAACTGCAGCCTGTACAGGAGCTGTGGGGACTGCCTCCT
CGCCCGGGACCCCTACTGTGCTTGGAGCGGCTCCAGCTGCAAGCACGTCAGCCTCTACCAGCCTCAGCTG
GCCACCAGGCCGTGGATCCAGGACATCGAGGGAGCCAGCGCCAAGGACCTTTGCAGCGCGTCTTCGGTTG
TGTCCCCGTCTTTTGTACCAACAGGGGAGAAGCCATGTGAGCAAGTCCAGTTCCAGCCCAACACAGTGAA
CACTTTGGCCTGCCCGCTCCTCTCCAACCTGGCGACCCGACTCTGGCTACGCAACGGGGCCCCCGTCAAT
GCCTCGGCCTCCTGCCACGTGCTACCCACTGGGGACCTGCTGCTGGTGGGCACCCAACAGCTGGGGGAGT
TCCAGTGCTGGTCACTAGAGGAGGGCTTCCAGCAGCTGGTAGCCAGCTACTGCCCAGAGGTGGTGGAGGA
CGGGGTGGCAGACCAAACAGATGAGGGTGGCAGTGTACCCGTCATTATCAGCACATCGCGTGTGAGTGCA
CCAGCTGGTGGCAAGGCCAGCTGGGGTGCAGACAGGTCCTACTGGAAGGAGTTCCTGGTGATGTGCACGC
TCTTTGTGCTGGCCGTGCTGCTCCCAGTTTTATTCTTGCTCTACCGGCACCGGAACAGCATGAAAGTCTT
CCTGAAGCAGGGGGAATGTGCCAGCGTGCACCCCAAGACCTGCCCTGTGGTGCTGCCCCCTGAGACCCGC
CCACTCAACGGCCTAGGGCCCCCTAGCACCCCGCTCGATCACCGAGGGTACCAGTCCCTGTCAGACAGCC
CCCCGGGGTCCCGAGTCTTCACTGAGTCAGAGAAGAGGCCACTCAGCATCCAAGACAGCTTCGTGGAGGT
ATCCCCAGTGTGCCCCCGGCCCCGGGTCCGCCTTGGCTCGGAGATCCGTGACTCTGTGGTGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_020210 unedited
TGGCAAGGGGGACGAGTCAGTGGACACTCCAGGAAGAGCGGCCCCGCGGGGGGCGATGAC
CGTGCGCTGACCCTGACTCACTCCAGGTCCGGAGGCGGGGGCCCCCGGGGCGACTCGGGG
GCGGACCGCGGGGCGGAGCTGCCGCCCGTGAGTCCGGCCGAGCCACCTGAGCCCGAGCCG
CGGGACACCGTCGCTCCTGCTCTCCGAATGCTGCGCACCGCGATGGGCCTGAGGAGCTGG
CTCGCCGCCCCATGGGGCGCGCTGCCGCCTCGGCCACCGCTGCTGCTGCTCCTGCTGCTG
CTGCTCCTGCTGCAGCCGCCGCCTCCGACCTGGGCGCTCAGCCCCCGGATCAGCCTGCCT
CTGGGCTCTGAAGAGCGGCCATTCCTCAGATTCGAAGCTGAACACATCTCCAACTACACA
GCCCTTCTGCTGAGCAGNGGATGGCAGGACCCTGGTACGTGGGTGGCTCGAGAGGCCCTC
TTTGCACTCAGTAGCAACCTCAGCTTCCTGCCAGGCGGGGGAGTACCAGGAGAACATGGA
GAACTTCACCCTGGCAGGGACGAGAGGGGGGAATGTCCTCCTGGAGATGGGAAGGNNNCC
GTTGTCCCTTCGACCCGAATTTCAGTCCCTGCCCTGGNGGGNTGATGGCGAGCTCTACAC
TGGNACAGTCAGCAGCTTCCAAGGAATGACCCCGGCTNNTGCGGAGCCAAAGCTTCGCCC
CACAAGACCAGAGCTCCTTAACGGGCTGAGACCCACTTTTTGTGGCCCAACCTACATTCT
GAAGACTGGGCACTTGCAGGCCATGATGACAAATT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_020210 unedited
NAAATCTNTGNNACCGCGGCCGCAATCTAGNATCGCGTTTTTTTTTTTTTTTTTTAAACA
GTAATTCTTCATACTTTATTAAAAAATGACATAAAGTGCATCTTATTAAAAAATATATAA
AAACCACATAAATTCAGGGCCCCTGTGCTGGGCAGTGTTGATATCCCTTAGAGTGGAGGA
AGGTGAGGGATGGAGGGTGAACTGGGGACTGGGGAGAGGACCAGGGTGCAGTTAGTTCCT
CGTGTTTGAGTTCAAAGATGGAGCGAGGGTGGATATGGTGGGAAGGGGCACACGGGTTCT
CACGCAACAACGGAGGAAGGCAGGCGACAGTCTCTTCCCTGAATTCTGAGGGAAACGCGT
ACATTGTCCCGAAATCTCTCCTGAGCTCGCGCTGTCCTCTCGTGTGGCCACAGCCTGATA
CAGGCTGGAAAGGTCCAGGAGTTGGGTCCGAGCCCAGGACCTGGTGAGGGCCGCAGCTCC
AACCTAGCCCTCTGGTCCCTGAGGTGGTGGGACGACGGCAGCAACAACTACATCCTCGGC
TGACTGGCAAGGCAGAACGCACGCAGCCCAAGCTGGTCCTGAATCTGCAGTGAGACAGGG
CACCCGGTGGCAGCGGGATAATGTGGAAGGTGAAGAGGCCAGTTGGTGAGGGTTTCCAAC
TCTGTCTCAGACACCCCTTTGGATGCATCCCCAGCACCCTGGGAAGCCTGAACTGGAGCA
CCCGGGGAGCTGATGCCATGCTATCCCTTCCTTTAATCCAATTTCCATCTGAAATGCTTC
CTCACCACGGCCTCCCTTACATACGAATATTGTTCTACAACCTCGCTGCCCCCCCTACTT
CTTTCACCTTCTGGCCCATTTTCTCTGATTCCCCGTCCCCCCGCCTTNCTCCCCTTCTTC
CCCTTTT
Restriction Sites NotI-NotI |
ACCN NM_020210
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020210.2, NP_064595.2
RefSeq Size 3843 bp
RefSeq ORF 2514 bp
Locus ID 10509
UniProt ID Q9NPR2
Cytogenetics 15q26.1
Protein Families Transmembrane
Protein Pathways Axon guidance
Summary Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 are predicted to encode the same protein which is annotated as the largest open reading frame. Translation may intitiate at either of the first two AUG sites. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_020210" in other vectors (4)
SKU Description Size Price
RC216548 SEMA4B (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (SEMA4B), transcript variant 1 10 ug
$766.00
RC216548L3 Lenti-ORF clone of SEMA4B (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (SEMA4B), transcript variant 1 10 ug
$1,066.00
RC216548L4 Lenti-ORF clone of SEMA4B (mGFP-tagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (SEMA4B), transcript variant 1 10 ug
$1,066.00
RG216548 SEMA4B (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (SEMA4B), transcript variant 1 10 ug
$789.00 $966.00

file-alt Citations

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