RASSF2 (NM_170774) Human Untagged Clone

SKU SC127100

RASSF2 (untagged)-Human Ras association (RalGDS/AF-6) domain family member 2 (RASSF2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RASSF2
• Synonyms: CENP-34; RASFADIN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127100 sequence for NM_170774 edited (data generated by NextGen Sequencing); ATGGACTACAGCCACCAAACGTCCCTAGTCCCATGTGGACAAGATAAATACATTTCCAAA; AATGAACTTCTCTTGCATCTGAAGACCTACAACTTGTACTATGAAGGCCAGAATTTACAG; CTCCGGCACCGGGAGGAAGAAGACGAGTTCATTGTGGAGGGGCTCCTGAACATCTCCTGG; GGCCTGCGCCGGCCCATTCGCCTGCAGATGCAGGATGACAACGAACGCATTCGACCCCCT; CCATCCTCCTCCTCCTGGCACTCTGGCTGTAACCTGGGGGCTCAGGGAACCACTCTGAAG; CCCCTGACTGTGCCCAAAGTTCAGATCTCAGAGGTGGATGCCCCGCCGGAGGGTGACCAG; ATGCCAAGCTCCACAGACTCCAGGGGCCTGAAGCCCCTGCAGGAGGACACCCCACAGCTG; ATGCGCACACGCAGTGATGTTGGGGTGCGTCGCCGTGGCAATGTGAGGACGCCTAGTGAC; CAGCGGCGAATCAGACGCCACCGCTTCTCCATCAACGGCCATTTCTACAACCATAAGACA; TCCGTGTTCACACCAGCCTATGGCTCTGTCACCAACGTCCGCATCAACAGCACCATGACC; ACCCCACAGGTCCTGAAGCTGCTGCTCAACAAATTTAAGATTGAGAATTCAGCAGAGGAG; TTTGCCTTGTACGTGGTCCATACGAGTGGTGAGAAACAGAAGCTGAAGGCCACCGATTAC; CCGCTGATTGCCCGAATCCTCCAGGGCCCATGTGAGCAGATCTCCAAAGTGTTCCTAATG; GAGAAGGACCAGGTGGAGGAAGTCACCTACGACGTGGCCCAGTATATAAAGTTCGAGATG; CCGGTACTTAAAAGCTTCATTCAGAAGCTCCAGGAGGAAGAAGATTGGGAAGTAAAGAAG; CTGATGCGCAAGTACACCGTGCTCCGGCTAATGATTCGACAGAGGCTGGAGGAGATAGCC; GAGACCCCAGCAACAATCTGA; ; Clone variation with respect to NM_170774.1; 886 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170774 unedited; GGTCAAAATTTGTATACGACTCCTATAGGCGGCCGGATTCGCCGAGGCAAGCGAAGGGTG; GAGAGTGATATGAAGAGCGAGAGAAAAGAGAGGACAGCGGACGAGCAGATCCGGTATCTG; GAATCCCGGCGCCTAGAACGTGTTTTTCGGGAGAGCAAAGGCTGTGTCTACGGCAGGCTG; GGGATATAGCCTCTCCTTCCGATGAAAAGAGAAAGGAAGAATGGACTACAGCCACCAAAC; GTCCCTAGTCCCATGTGGACAAGATAAATACATTTCCAAAAATGAACTTCTCTTGCATCT; GAAGACCTACAACTTGTACTATGAAGGCCAGAATTTACAGCTCCGGCACCGGGAGGAAGA; AGACGAGTTCATTGTGGAGGGGCTCCTGAACATCTCCTGGGGCCTGCGCCGGCCCATTCG; CCTGCAGATGCAGGATGACAACGAACGCATTCGACCCCCTCCATCCTCCTCCTCCTGGCA; CTCTGGCTGTAACCTGGGGGCTCAGGGAACCACTCTGAAGCCCCTGACTGTGCCCAAAGT; TCAGATCTCAGAGGTGGATGCCCCGCCGGAGGGTGACCAGATGCCAAGCTCCACAGACTC; CAGGGGCCTGAAGCCCCTGCAGGAGGACACCCCACAGCTGATGCGCACACGCAGTGATGT; TGGGGTGCGTCGCCGTGGCAATGTGAGGACGCCTAGTGACCAGCGGCGAATCAGACGCCA; CCGCTTCTCCATCAACGGCCATTTCTACAACCATAAGACATCCGTGTTCACACCAGCCTA; TGGCTCTGTCACCAACGTCCGCATCAACAGCACCATGACCACCCCACAGGTCCTGAAGCT; GCTGCTCAACAATTTAGATTGAGAATCACAGNAGGAGTTGCTTGTACGTGGTCATACGAG; TGGTGAGAACAGAGCTGAAGCACCGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_170774 unedited; ACTATGGACCGCGGCACGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTCTTTGAAA; GTGCTTTATTTCAGTAAGATCTAAGTCAGATGCATTAAAACATATCAAAATGTTACAAAA; ATGTATGGCTCCCTTGCTGAGGCCCTGTCAGATTATGTTAGATGAGTAAACGCATCAGTG; TGTAAGTTCAGAACCAAACGTTGAATCAAGTCATGTACCATCGCTGAAAGGCAAGTTATA; ATTAAGAATACTTCACAAGGCTAAAACAATTGGGAAAACCCTTCAGAAACGCTAGCAATT; ACACACCAGAAACATCAGCCTGGAAACAGGTCTGCGATCTGCAGGGAAGACTCCAAAGGT; AAACTGAGGGTTTCCCACCACAGCTTGTGCATCTTGTGCTCAGTTAAATACAGTTAAAAC; ACAAGGGGCGCTTCAACACCCCCCTTGTGATAGGGCAGAGCTTTCTATTACATCTGGAAG; AAGGGGGCGCCACATTCATTCTTATTTTTACATCTCTAGGAAATCATATCTCATCTCATC; TGAGCGAAGCAAGACTTTGTGCAAAACGATTTAAGTGGAAACGTGAAAATGTGTGCTGCT; ATATCAGGGACGCTTATGGAGATCTCCTGTCTCCTATCTCATTCTTCACGTCATGGTTTG; GCCGCTGTAAGACACTGAAATTGCCTTGTTCATGTAGAAATTTCTCTTTTGGCTGGGCAC; AGTGGCTCACGCCTGTAATCCCAACACTTNTGGAGGCCTAGGCGGNTGGNATCACGAGTC; AGGAGTTCAAGACAGCCTGNCCAAGATGTGAAACCCCACCCTCTACTAAACCACACAAAA; AATAGCCGGGGCGTGTGGCGGGTCCCTGTTATTCCAGCTACTTGGGAGGGCTGAGACAGA; AATCACTTGNACCCANAGCGGATGNTGCANTGAGCTGAGATCCACCCCTGCCCTCAGCCT; GGGAACAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_170774
• Insert Size: 5480 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_170774.1, NP_739580.1
• RefSeq Size: 5307 bp
• RefSeq ORF: 981 bp
• Locus ID: 9770
• UniProt ID: P50749
• Cytogenetics: 20p13
• Summary: This gene encodes a protein that contains a Ras association domain. Similar to its cattle and sheep counterparts, this gene is located near the prion gene. Two alternatively spliced transcripts encoding the same isoform have been reported. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform.