Nck beta (NCK2) (NM_003581) Human Untagged Clone

SKU SC126794

NCK2 (untagged)-Human NCK adaptor protein 2 (NCK2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Nck beta
• Synonyms: GRB4; NCKbeta
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003581, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGCCCAGCAGGACCAGGAGCTGGACATCA; AGAAGAACGAGCGGCTGTGGTTGCTGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCAACAG; GACGGGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGGCTCCCTCGTGAAGAAC; CTGAAGGACACACTAGGCCTCGGCAAGACGCGCAGGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCA; GCACGGACGCCGAGTACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCCGGCCTT; CGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCCTGGTGAAGGGGTCGCGCGTCACCGTC; ATGGAGAAGTGCAGCGACGGTTGGTGGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACT; ACGTCTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCCTGCGCAAGGGCGCCTC; GCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCATGTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACC; GAGGAGGAGCTCAACTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACCCCGAGT; GGTGGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCCAAAAACTACGTGGTGGTCCTCAGTGA; CGGGCCTGCCCTGCACCCTGCGCACGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTC; GCGGGCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCCCTCAACGAGCGGGGCG; TGGAGGGCGACTTCCTCATTAGGGACAGCGAGTCCTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTC; AGGGAAGAACAAACACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGCTTCCAC; ACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTTCACCAGCGAGCACGGGGAGAAGCTCT; ACCTCGTCAGGGCCCTGCAGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003581 unedited; TGTAACTTCAGAGGTGTAATTAGCTGAAAACATCATCGTTTTGAAGAGTTCTGCGTTTTG; CCAGTCACCTCTCAACTGTGTGCCAAAGAAGGACTCCATGAAAGATGACAGAAGAAGTTA; TTGTGATAGCCAAGTGGGACTACACCGCCCAGCAGGACCAGGAGCTGGACATCAAGAAGA; ACGAGCGGCTGTGGTTGCTGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCA; ACAGGACGGGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGGCT; CCCTCGTGAAGAACCTGAAGGACACACTAGGCCTCGGCAAGACGCGCAGGAAGACCAGCG; CGCGGGATGCGTCCCCCACGCCCAGCACGGACGCCGAGTACCCCGCCAATGGCAGCGGCG; CCGACCGCATCTACGACCTCAACATCCCGGCCTTCGTCAAGTTCGCCTATGTGGCCGAGC; GGGAGGATGAGTTGTCCCTGGTGAAGGGGTCGCGCGTCACCGTCATGGAGAAGTGCAGCG; ACGGTTGGTGGCGGNGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACTACGTCT; TGGAGGAAGTGGACGAAGCGGCTGCGGAGTCCCCAGCTTCCTGAGCCTGCGCAAGGCGCC; TCCCTGAGCATGGCCAGGGCTCCGCGTGCTGAATGTGTCCAAACGCTTACCCCTTCACTT; TATCCCGGAGGAGACCTAACTTTCAAAAGGGGAAACATGGAGTGATTGAAAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003581 unedited; NAAATTTGACTTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTCC; TTGGCATAATACTTTTTTTTATTACAATATCCAAAAAACTGGGTATGCAAGTTTAGGGGA; TCTCAAGACCCCTTCTTCAATCGTAGGAATGTGCCATCTCAAGACGTTATATTTAAACTA; TAAAGAAGTTCAAATGTAAAGGAAAAAGAAAATGCAATTTCTTCATAAACATTCTGTGTG; TCTCTTACTACCAATCACATACTCTTTTGTAAACCCTGAAAAATTTCCCTGTAAATAATA; TATATATATATAGTGTGTGTGTGTGTGTGTGTGTGTGCACGTGTGTGTGTGTATAAAGTG; TTGGTAGCTCCCTTCCCCAAAGATCAGCTGTTTCCCTTAATCATCTGTATTAGTGTCAAC; AAACGGCTACAGATACATATTTCTTTGAGAATTAAATACATAATTGTGAGATTCACACAA; GCCGAAGGGCAAGAGGAAAAAGCACTATGTGGATGGCACATTTGGGGTAAATTACCAAAA; CATCTGTCTTTCTGAGTCACCGGTTCTCTCTTTTGCAGACTGCCTTTCCAGGAAATTCTT; TCTTTATTGCAAGCACAGCCCCAAAGGCACTGGGCAGCCCCCTCACTGCCATCGCACCCG; GTGGCTCCAGCATTGCTATCACCAAACCTTTCGGCTCCCTGCCACACCCGGGTACCCCTG; AAGCGCCACAGGACATGNGCCGAGGCCTGCTAGGACTGGGGCCCCCGCCCCTGTTTTCCC; CACCCCTCTCGACGCCCCACAAACCGACGCCTGCCCTGGCGGCCCCCCAGGCCTCCCCCT; CGCCCTCCCGTCTCCCTCCTTCCTTCCCAGGGCGCCCTCCTCTCCTCTCACTATCCCCGC; CCCCCCACCCCCCTCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003581
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003581.2, NP_003572.2
• RefSeq Size: 2698 bp
• RefSeq ORF: 1143 bp
• Locus ID: 8440
• UniProt ID: O43639
• Cytogenetics: 2q12.2
• Domains: SH2, SH3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, ErbB signaling pathway, Pathogenic Escherichia coli infection, T cell receptor signaling pathway
• Summary: This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (A). Variants 1 and 2 both encode isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.