Hemoglobin subunit epsilon (HBE1) (NM_005330) Human Untagged Clone

SKU SC126296

HBE1 (untagged)-Human hemoglobin, epsilon 1 (HBE1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Hemoglobin subunit epsilon
• Synonyms: HBE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005330 edited; GCCATGGTTGTGAGGCCTCCCCAGCTATGTGGAACTAGACTTCAAGAAGTTAAGCAACTT; GCTCAAGATCATCTAAGAAATAGATGAGGAGCCAACAAAAAAGAGCCTCAGGATCCAGCA; CACATTATCACAAACTTAGTGTCCATCCATCACTGCTGACCCTCTCCGGACCTGACTCCA; CCCCTGAGGGACACAGGTCAGCCTTGACCAATGACTTTTAAGTACCATGGAGAACAGGGG; GCCAGAACTTCGGCAGTAAAGAATAAAAGGCCAGACAGAGAGGCAGCAGCACATATCTGC; TTCCGACACAGCTGCAATCACTAGCAAGCTCTCAGGCCTGGCATCATGGTGCATTTTACT; GCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGAATGTGGAAGAGGCTGGA; GGTGAAGCCTTGGGCAGACTCCTCGTTGTTTACCCCTGGACCCAGAGATTTTTTGACAGC; TTTGGAAACCTGTCGTCTCCCTCTGCCATCCTGGGCAACCCCAAGGTCAAGGCCCATGGC; AAGAAGGTGCTGACTTCCTTTGGAGATGCTATTAAAAACATGGACAACCTCAAGCCCGCC; TTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG; CTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCT; GAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATTGCCCTGGCCCATAAG; TACCACTGAGTTCTCTTCCAGTTTGCAGGTGTTCCTGTGACCCTGACACCCTCCTTCTGC; ACATGGGGACTGGGCTTGGCCTTGAGAGAAAGCCTTCTGTTTAATAAAGTACATTTTCTT; CAGTAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005330 unedited; NGGGGGTTCGCGATTTTGTAATACGACTTACTATAGGCGGCCGCGCAATTCGATCTGGTA; CCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGGCCATGG; TTGTGAGGCCTCCCCAGCTATGTGGAACTAGACTTCAAGAAGTTAAGCAACTTGCTCAAG; ATCATCTAAGAAATAGATGAGGAGCCAACAAAAAAGAGCCTCAGGATCCAGCACACATTA; TCACAAACTTAGTGTCCATCCATCACTGCTGACCCTCTCCGGACCTGACTCCACCCCTGA; GGGACACAGGTCAGCCTTGACCAATGACTTTTAAGTACCATGGAGAACAGGGGGCCAGAA; CTTCGGCAGTAAAGAATAAAAGGCCAGACAGAGAGGCAGCAGCACATATCTGCTTCCGAC; ACAGCTGCAATCACTAGCAAGCTCTCAGGCCTGGCATCATGGTGCATTTTACTGCTGAGG; AGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGAATGTGGAAGAGGCTGGAGGTGAAG; CCTTGGGCAGACTCCTCGTTGTTTACCCCTGGACCCAGAGATTTTTTGACAGCTTTGGAA; ACCTGTCGTCTCCCTCTGCCATCCTGGGCAACCCCAAGGTCAAGGCCCATGGCAAGAAGG; TGCTGACTTCCTTTGGAGATGCTATTAAAAACATGGACCACCTCAAGCCCGCCTTTGCTA; AGCTGAGTGAGCTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACCTCAAGCTCCTGG; GTAACGTGATGGTGATTATTCTGGCTACTCACTTTGGCCGGNAGTTCACCCCTGAAGTGC; AGGCTGCCTGGCAAAACTGGTGTCTGCTGTCGCCATGGCCCTGCCCCTAAGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005330
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005330.3, NP_005321.1
• RefSeq Size: 816 bp
• RefSeq ORF: 444 bp
• Locus ID: 3046
• UniProt ID: P02100
• Cytogenetics: 11p15.4
• Summary: The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008]