ADAM12 (NM_003474) Human Untagged Clone

SKU SC125537

ADAM12 (untagged)-Human ADAM metallopeptidase domain 12 (ADAM12), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ADAM12
• Synonyms: ADAM12-OT1; CAR10; MCMP; MCMPMltna; MLTN; MLTNA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003474, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCAGCGCGCCCGCTGCCCGTGTCCCCCGCCCGCGCCCTCCTGCTCGCCCTGGCCGGTGCTCTGCTCG; CGCCCTGCGAGGCCCGAGGGGTGAGCTTATGGAACCAAGGAAGAGCTGATGAAGTTGTCAGTGCCTCTGT; TGGGAGTGGGGACCTCTGGATCCCAGTGAAGAGCTTCGACTCCAAGAATCATCCAGAAGTGCTGAATATT; CGACTACAACGGGAAAGCAAAGAACTGATCATAAATCTGGAAAGAAATGAAGGTCTCATTGCCAGCAGTT; TCACGGAAACCCACTATCTGCAAGACGGTACTGATGTCTCCCTCGCTCGAAATTACACGGTAATTCTGGG; TCACTGTTACTACCATGGACATGTACGGGGATATTCTGATTCAGCAGTCAGTCTCAGCACGTGTTCTGGT; CTCAGGGGACTTATTGTGTTTGAAAATGAAAGCTATGTCTTAGAACCAATGAAAAGTGCAACCAACAGAT; ACAAACTCTTCCCAGCGAAGAAGCTGAAAAGCGTCCGGGGATCATGTGGATCACATCACAACACACCAAA; CCTCGCTGCAAAGAATGTGTTTCCACCACCCTCTCAGACATGGGCAAGAAGGCATAAAAGAGAGACCCTC; AAGGCAACTAAGTATGTGGAGCTGGTGATCGTGGCAGACAACCGAGAGTTTCAGAGGCAAGGAAAAGATC; TGGAAAAAGTTAAGCAGCGATTAATAGAGATTGCTAATCACGTTGACAAGTTTTACAGACCACTGAACAT; TCGGATCGTGTTGGTAGGCGTGGAAGTGTGGAATGACATGGACAAATGCTCTGTAAGTCAGGACCCATTC; ACCAGCCTCCATGAATTTCTGGACTGGAGGAAGATGAAGCTTCTACCTCGCAAATCCCATGACAATGCGC; AGCTTGTCAGTGGGGTTTATTTCCAAGGGACCACCATCGGCATGGCCCCAATCATGAGCATGTGCACGGC; AGACCAGTCTGGGGGAATTGTCATGGACCATTCAGACAATCCCCTTGGTGCAGCCGTGACCCTGGCACAT; GAGCTGGGCCACAATTTCGGGATGAATCATGACACACTGGACAGGGGCTGTAGCTGTCAAATGGCGGTTG; AGAAAGGAGGCTGCATCATGAACGCTTCCACCGGGTACCCATTTCCCATGGTGTTCAGCAGTTGCAGCAG; GAAGGACTTGGAGACCAGCCTGGAGAAAGGAATGGGGGTGTGCCTGTTTAACCTGCCGGAAGTCAGGGAG; TCTTTCGGGGGCCAGAAGTGTGGGAACAGATTTGTGGAAGAAGGAGAGGAGTGTGACTGTGGGGAGCCAG; AGGAATGTATGAATCGCTGCTGCAATGCCACCACCTGTACCCTGAAGCCGGACGCTGTGTGCGCACATGG; GCTGTGCTGTGAAGACTGCCAGCTGAAGCCTGCAGGAACAGCGTGCAGGGACTCCAGCAACTCCTGTGAC; CTCCCAGAGTTCTGCACAGGGGCCAGCCCTCACTGCCCAGCCAACGTGTACCTGCACGATGGGCACTCAT; GTCAGGATGTGGACGGCTACTGCTACAATGGCATCTGCCAGACTCACGAGCAGCAGTGTGTCACGCTCTG; GGGACCAGGTGCTAAACCTGCCCCTGGGATCTGCTTTGAGAGAGTCAATTCTGCAGGTGATCCTTATGGC; AACTGTGGCAAAGTCTCGAAGAGTTCCTTTGCCAAATGCGAGATGAGAGATGCTAAATGTGGAAAAATCC; AGTGTCAAGGAGGTGCCAGCCGGCCAGTCATTGGTACCAATGCCGTTTCCATAGAAACAAACATCCCCCT; GCAGCAAGGAGGCCGGATTCTGTGCCGGGGGACCCACGTGTACTTGGGCGATGACATGCCGGACCCAGGG; CTTGTGCTTGCAGGCACAAAGTGTGCAGATGGAAAAATCTGCCTGAATCGTCAATGTCAAAATATTAGTG; TCTTTGGGGTTCACGAGTGTGCAATGCAGTGCCACGGCAGAGGGGTGTGCAACAACAGGAAGAACTGCCA; CTGCGAGGCCCACTGGGCACCTCCCTTCTGTGACAAGTTTGGCTTTGGAGGAAGCACAGACAGCGGCCCC; ATCCGGCAAGCAGATAACCAAGGTTTAACCATAGGAATTCTGGTGACCATCCTGTGTCTTCTTGCTGCCG; GATTTGTGGTTTATCTCAAAAGGAAGACCTTGATACGACTGCTGTTTACAAATAAGAAGACCACCATTGA; AAAACTAAGGTGTGTGCGCCCTTCCCGGCCACCCCGTGGCTTCCAACCCTGTCAGGCTCACCTCGGCCAC; CTTGGAAAAGGCCTGATGAGGAAGCCGCCAGATTCCTACCCACCGAAGGACAATCCCAGGAGATTGCTGC; AGTGTCAGAATGTTGACATCAGCAGACCCCTCAACGGCCTGAATGTCCCTCAGCCCCAGTCAACTCAGCG; AGTGCTTCCTCCCCTCCACCGGGCTCCACGTGCACCTAGCGTCCCTGCCAGACCCCTGCCAGCCAAGCCT; GCACTTAGGCAGGCCCAGGGGACCTGTAAGCCAAACCCCCCTCAGAAGCCTCTGCCTGCAGATCCTCTGG; CCAGAACAACTCGGCTCACTCATGCCTTGGCCAGGACCCCAGGACAATGGGAGACTGGGCTCCGCCTGGC; ACCCCTCAGACCTGCTCCACAATATCCACACCAAGTGCCCAGATCCACCCACACCGCCTATATTAAGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003474 unedited; CGTATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCGGCGCGGGCGCT; GCGCGAGGGCTCCGGAGCTGACTCGCCGAGGCAGGAAATCCCTCCGGTCGCGACGCCCGG; CCCCGGCTCGGCGCCCGCGTGGGATGGTGCAGCGCTCGCCGCCGGGCCCGAGAGCTGCTG; CACTGAAGGCCGGCGACGATGGCAGCGCGCCCGCTGCCCGTGTCCCCCGCCCGCGCCCTC; CTGCTCGCCCTGGCCGGTGCTCTGCTCGCGCCCTGCGAGGCCCGAGGGGTGAGCTTATGG; AACCAAGGAAGAGCTGATGAAGTTGTCAGTGCCTCTGTTGGGAGTGGGGACCTCTGGATC; CCAGTGAAGAGCTTCGACTCCAAGAATCATCCAGAAGTGCTGAATATTCGACTACAACGG; GAAAGCAAAGAACTGATCATAAATCTGGAAAGAAATGAAGGTCTCATTGCCAGCAGTTTC; ACGGAAACCCACTATCTGCAAGACGGTACTGATGTCTCCCTCGCTCGAAATTACACGGGT; CACTGTTACTACCATGGACATGTACGGGGATATTCTGATTCAGCAGTCAGTCTCAGCACG; TGTTCTGGTCTCAGGGGACTTATTGTGTTTGAAAATGAAAGCTATGTCTTANAACCAATG; AAAAGTGCAACCAACAGATACAAACTCTTCCCAGCGAAGAAGCTGAAAAGCGTCCGGNGA; TCATGTGGATCACATCACAACACACCAAACCTCGCTGCANAGAATGTGTTTCCACCACCC; TCTCAGACATGGGCAAGAAAGCATAAAAGAGAGACCCTCAAGGCACTAAGTATGTGGAGC; TGGTGATCGTGGCAGACAACCGAGAGCTTCAGAGGCAGGNANNAGATCTGCAAAAAGCTA; GCAGCGATAATAGAGATGCTAATCACGTGACAGTNTACGACACTGACANTCGNACGTGTG; GTAGCNTGGAATGTAATGACTGGCAATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_003474
• Insert Size: 3050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003474.3, NP_003465.3
• RefSeq Size: 5504 bp
• RefSeq ORF: 2730 bp
• Locus ID: 8038
• UniProt ID: O43184
• Cytogenetics: 10q26.2
• Domains: ACR, DISIN, Pep_M12B_propep, Reprolysin
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Summary: This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as ADAM12-Lb). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.