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CYP21A2 (NM_000500) Human Untagged Clone

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SKU
SC125487
CYP21A2 (untagged)-Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CYP21A2
Synonyms CA21H; CAH1; CPS1; CYP21; CYP21B; P450c21B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000500 edited
GTCAAGGGAGGCCCCAAAACAGTCTACACAGCAGGAGGGATGGCTGGGGCTCTTGAGCTA
TAAGTGGCACCTCAGGGCCCTGACGGGCGTCTCGCCATGCTGCTCCTGGGCCTGCTGCTG
CTGCTGCCCCTGCTGGCTGGCGCCCGCCTGCTGTGGAACTGGTGGAAGCTCCAGAGCCTC
CACCTCCCGCCTCTTGCCCCGGGCTTCTTGCACTTGCTGCAGCCCGACCTCCCAATCTAT
CTGCTTGGCCTGACTCAGAAATTCGGGCCCATCTACAGGCTCCACCTTGGGCTGCAAGAT
GTGGTGGTGCTGAACTCCAAGAGGACCATTGAGGAAGCCATGGTCAAAAAGTGGGCAGAC
TTTGCTGGCAGACCTGAGCCACTTACCTACAAGCTGGTGTCTAGGAACTACCCGGACCTG
TCCTTGGGAGACTACTCCCTGCTCTGGAAAGCCCACAAGAAGCTCACCCGCTCAGCCCTG
CTGCTGGGCATCCGTGACTCCATGGAGCCAGTGGTGGAGCAGCTGACCCAGGAGTTCTGT
GAGCGCATGAGAGCCCAGCCCGGCACCCCTGTGGCCATTGAGGAGGAATTCTCTCTCCTC
ACCTGCAGCATCATCTGTTACCTCACCTTCGGAGACAAGATCAAGGACGACAACTTAATG
CCTGCCTATTACAAATGTATCCAGGAGGTGTTAAAAACCTGGAGCCACTGGTCCATCCAA
ATTGTGGACGTGATTCCCTTTCTCAGGTTCTTCCCCAATCCAGGTCTCCGGAGGCTGAAG
CAGGCCATAGAGAAGAGGGATCACATCGTGGAGATGCAGCTGAGGCAGCACAAGGAGAGC
CTCGTGGCAGGCCAGTGGAGGGACATGATGGACTACATGCTCCAAGGGGTGGCGCAGCCG
AGCATGGAAGAGGGCTCTGGACAGCTCCTGGAAGGGCACGTGCACATGGCTGCAGTGGAC
CTCCTGATCGGTGGCACTGAGACCACAGCAAACACCCTCTCCTGGGCCGTGGTTTTTTTG
CTTCACCACCCTGAGATTCAGCAGCGACTGCAGGAGGAGCTAGACCACGAACTGGGCCCT
GGTGCCTCCAGCTCCCGGGTCCCCTACAAGGACCGTGCACGGCTGCCCTTGCTCAATGCC
ACCATCGCCGAGGTGCTGCGCCTGCGGCCCGTTGTGCCCTTAGCCTTGCCCCACCGCACC
ACACGGCCCAGCAGCATCTCCGGCTACGACATCCCTGAGGGCACAGTCATCATTCCGAAC
CTCCAAGGCGCCCACCTGGATGAGACGGTCTGGGAGAGGCCACATGAGTTCTGGCCTGAT
CGCTTCCTGGAGCCAGGCAAGAACTCCAGAGCTCTGGCCTTCGGCTGCGGTGCCCGCGTG
TGCCTGGGCGAGCCGCTGGCGCGCCTGGAGCTCTTCGTGGTGCTGACCCGACTGCTGCAG
GCCTTCACGCTGCTGCCCTCCGGGGACGCCCTGCCCTCCCTGCAGCCCCTGCCCCACTGC
AGTGTCATCCTCAAGATGCAGCCTTTCCAAGTGCGGCTGCAGCCCCGGGGGATGGGGGCC
CACAGCCCGGGCCAGAGCCAGTGATGGGGCAGGACCGATGCCAGCCGGGTACCTCAGTTT
CTCCTTTATTGCTCCTGTACGAACCCCTCCCCTCCCCCCTGTAAACACAGTGCTGCGAGA
TCGCTGGCAGAGAAGGCTTCCTCCAGCGGCTGGGTGGTGAAGGACCCTGGCTCTTCTCTC
GGGGCGACCCCTCAGTGCTCGGCAGTCATACTGGGGTGCGAGAGAGGTGGGCAGCAGCTC
AGCCTCCCCCCGCTGGGGAGCGAAAGTTTCTTGGTCTCAGCTTCATTTCCGTGAAGGGCA
CCGAGAACTCGAAGCCCTTCCAGTGGTACCAGCTCACTCCCTGGGAAAGGGGTTGTCAAG
AGAGAGTCAAAGCCGGATGTCCCATCTGCTCTTCCCGTTCCCCTT
Restriction Sites Please inquire |
ACCN NM_000500
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000500.5, NP_000491.2
RefSeq Size 2114 bp
RefSeq ORF 1488 bp
Locus ID 1589
UniProt ID P08686
Cytogenetics 6p21.33
Domains p450
Protein Families Druggable Genome, P450
Protein Pathways C21-Steroid hormone metabolism, Metabolic pathways
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) encodes the longer isoform (a).
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Documents
Resources
"NM_000500" in other vectors (6)
SKU Description Size Price
RC216416 CYP21A2 (Myc-DDK-tagged)-Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1 10 ug
$457.00
RC216416L1 Lenti ORF clone of Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC216416L2 Lenti ORF clone of Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1, mGFP tagged 10 ug
$757.00
RC216416L3 Lenti ORF clone of Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC216416L4 Lenti ORF clone of Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1, mGFP tagged 10 ug
$757.00
RG216416 CYP21A2 (tGFP-tagged) - Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1 10 ug
$489.00 $657.00

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