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PHD4 (P4HTM) (NM_177939) Human Untagged Clone

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SKU
SC125461
P4HTM (untagged)-Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1
  $468.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PHD4
Synonyms EGLN4; HIDEA; HIFPH4; P4H-TM; PH-4; PH4; PHD4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_177939 edited
ATGGCGGCAGCGGCGGTGACAGGCCAGCGGCCTGAGACCGCGGCGGCCGAGGAGGCCTCG
AGGCCGCAGTGGGCGCCGCCAGACCACTGCCAGGCTCAGGCGGCGGCCGGGCTGGGCGAC
GGCGAGGACGCACCGGTGCGTCCGCTGTGCAAGCCCCGCGGCATCTGCTCGCGCGCCTAC
TTCCTGGTGCTGATGGTGTTCGTGCACCTGTACCTGGGTAACGTGCTGGCGCTGCTGCTC
TTCGTGCACTACAGCAACGGCGACGAAAGCAGCGATCCCGGGCCCCAACACCGTGCCCAG
GGCCCCGGGCCCGAGCCCACCTTAGGTCCCCTCACCCGGCTGGAGGGCATCAAGGTGGGG
CACGAGCGTAAGGTCCAGCTGGTCACCGACAGGGATCACTTCATCCGAACCCTCAGCCTC
AAGCCGCTGCTCTTCGAAATCCCCGGCTTCCTGACTGATGAAGAGTGTCGGCTCATCATC
CATCTGGCGCAGATGAAGGGGTTACAGCGCAGCCAGATCCTGCCTACTGAAGAGTATGAA
GAGGCAATGAGCACTATGCAGGTCAGCCAGCTGGACCTCTTCCGGCTGCTGGACCAGAAC
CGTGATGGGCACCTTCAGCTCCGTGAGGTTCTGGCCCAGACTCGCCTGGGAAATGGATGG
TGGATGACTCCAGAGAGCATTCAGGAGATGTACGCCGCGATCAAGGCTGACCCTGATGGT
GACGGAGTGCTGAGTCTGCAGGAGTTCTCCAACATGGACCTTCGGGACTTCCACAAGTAC
ATGAGGAGCCACAAGGCAGAGTCCAGTGAGCTGGTGCGGAACAGCCACCATACCTGGCTC
TACCAGGGTGAGGGTGCCCACCACATCATGCGTGCCATCCGCCAGAGGGTGCTGCGCCTC
ACTCGCCTGTCGCCTGAGATCGTGGAGCTCAGCGAGCCGCTGCAGGTTGTTCGATATGGT
GAGGGGGGCCACTACCATGCCCACGTGGACAGTGGGCCTGTGTACCCAGAGACCATCTGC
TCCCATACCAAGCTGGTAGCCAACGAGTCTGTACCCTTCGAGACCTCCTGCCGCTACATG
ACAGTGCTGTTTTATTTGAACAACGTCACTGGTGGGGGCGAGACTGTTTTCCCTGTAGCA
GATAACAGAACCTACGATGAAATGAGTCTGATTCAGGATGACGTGGACCTCCGTGACACA
CGGAGGCACTGTGACAAGGGAAACCTGCGTGTCAAGCCCCAACAGGGCACAGCAGTCTTC
TGGTACAACTACCTGCCTGATGGGCAAGGTTGGGTGGGTGACGTAGACGACTACTCGCTG
CACGGGGGCTGCCTGGTCACGCGCGGCACCAAGTGGATTGCCAACAACTGGATTAATGTG
GACCCCAGCCGAGCGCGGCAAGCGCTGTTCCAACAGGAGATGGCCCGCCTTGCCCGAGAA
GGGGGCACCGACTCACAGCCCGAGTGGGCTCTGGACCGGGCCTACCGCGATGCGCGCGTG
GAACTCTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_177939 unedited
GTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGCCATGGCGGCAGCGG
CGGTGACAGGCCAGCGGCCTGAGACCGCGGCGGCCGAGGAGGCCTCGAGGCCGCAGTGGG
CGCCGCCAGACCACTGCCAGGCTCAGGCGGCGGCCGGGCTGGGCGACGGCGAGGACGCAC
CGGTGCGTCCGCTGTGCAAGCCCCGCGGCATCTGCTCGCGCGCCTACTTCCTGGTGCTGA
TGGTGTTCGTGCACCTGTACCTGGGTAACGTGCTGGCGCTGCTGCTCTTCGTGCACTACA
GCAACGGCGACGAAAGCAGCGATCCCGGGCCCCAACACCGTGCCCAGGGCCCCGGGCCCG
AGCCCACCTTAGGTCCCCTCACCCGGCTGGAGGGCATCAAGGTGGGGCACGAGCGTAAGG
TCCAGCTGGTCACCGACAGGGATCACTTCATCCGAACCCTCAGCCTCAAGCCGCTGCTCT
TCGAAATCCCCGGCTTCCTGACTGATGAAGAGTGTCGGCTCATCATCCATCTGGCGCAGA
TGAAGGGGTTACAGCGCAGCCAGATCCTGCCTACTGAAGAGTATGAAGAGGCAATGAGCA
CTATGCAGGTCAGCCAGCTGGACCTCTTCCGGCTGCTGGACCAGAACCGTGATGGGCACC
TTCAGCTCCGTGAGGTTCTGGCCCAGACTCGCCTGGGAAATGGATGGTGGATGACTCCAG
AGAGCATTCAGGAGATGTACGCCGCGATCAAGGCTGACCCTGATGGTGACGGAGTGCTGA
GTCTGCAGGAGTTCTCCAACATGGGACCTCGGNNACTTCCACAGTACATGAGGAGCCACA
AGGCAGAGTCCAGTGAGCTGGTGCGGNACAGNCACCATACCTGGCTCTACCAGGGTGGAG
GGGTGCCACCACATCATGCGTGCCATCCGNCAGAGGGTGCTGCGGCTNNACTCGCTGTGC
CTGGN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_177939 unedited
ATGGACGCGGCCGCATTTTAGNGTCGAGTTTTTTTTTTTTTTTTTTCGAACCTTGTGGTT
TTTTATTTGACGGGGCAGATGGAGTCAGTACACAATAAATAACATGAATATAGGAACTGC
GCCGTATCGCGGCTGGCGGGGTGGGGCAAGACATTGGCCAGACCTTTAGTCTGCAGCAGG
ACAGAAGGACAGCCGGACCCCTGATCTTGGGCAACTGGCGACCCGCGGCTGGGAACCGGG
GCTAACTCTTCCCTCAGAGTTCCACGCGCGCATCGCGGTAGGCCCGGTCCAGAGCCCACT
CGGGCTGTGAGTCGGTGCCCCCTTCTCGGGCAAGGCGGGCCATCTCCTGTTGGAACAGCG
CTTGCCGCGCTCGGCTGGGGTCCACATTAATCCAGTTGTTGGCAATCCACTTGGTGCCGC
GCGTGACCAGGCAGCCCCCGTGCAGCGAGTAGTCGTCTACGTCACCCACCCAACCTTGCC
CATCAGGCAGGTAGTTGTACCAGAAGACTGCTGTGCCCTGTTGGGGCTTGACACGCAGGT
TTCCCTTGTCACAGTGCCTCCGTGTGTCACGGAGGTCCACGTCATCCTGAATCAGACTCA
TTTCATCGTAGGTTCTGTTATCTGCTACAGGGAAAACAGTCTCGCCCCCACCAGTGACGT
TGTTCAAATAAAACAGCACTGTCATGTAGCGGCAGGAGGTCTCGAAGGGTACAGACTCGT
TGGCTACCAGCTTGGTATGGGAGCAGATGGTCTCTGGGTACACAGGCCCACTGTCCACGT
GGGCATGGTAGTGGCCCCCCTCACCATATCGAACAACCTGCAGCGGCTCGCTGAGCTCCA
CGATCTCAGCGACAGGCGAGTGAGGCGCAGCACCCTCTGGCGTATGCACGCATGATGTGG
GTGGCACCCTCT
Restriction Sites NotI-NotI |
ACCN NM_177939
Insert Size 1880 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177939.2, NP_808808.1
RefSeq Size 2111 bp
RefSeq ORF 1509 bp
Locus ID 54681
UniProt ID Q9NXG6
Cytogenetics 3p21.3
Protein Families Druggable Genome, Transmembrane
Summary The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the shortest transcript.
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Documents
Resources
"NM_177939" in other vectors (6)
SKU Description Size Price
RC216367 P4HTM (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1 10 ug
$467.00
RC216367L1 Lenti ORF clone of Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, Myc-DDK-tagged 10 ug
$767.00
RC216367L2 Lenti ORF clone of Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, mGFP tagged 10 ug
$767.00
RC216367L3 Lenti ORF clone of Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, Myc-DDK-tagged 10 ug
$767.00
RC216367L4 Lenti ORF clone of Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, mGFP tagged 10 ug
$767.00
RG216367 P4HTM (tGFP-tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1 10 ug
$489.00 $667.00

file-alt Citations

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