IL2 (NM_000586) Human Untagged Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | IL2 |
| Synonyms | IL-2; lymphokine; TCGF |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_000586 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000586.2, NP_000577.2 |
| RefSeq Size | 1047 bp |
| RefSeq ORF | 462 bp |
| Locus ID | 3558 |
| UniProt ID | P60568 |
| Cytogenetics | 4q27 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus |
| Summary | This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. provided by RefSeq, Sep 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000586" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210013 | IL2 (Myc-DDK-tagged)-Human interleukin 2 (IL2) | 10 ug |
$150.00
|
|
| RC210013L1 | Lenti ORF clone of Human interleukin 2 (IL2), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210013L2 | Lenti ORF clone of Human interleukin 2 (IL2), mGFP tagged | 10 ug |
$450.00
|
|
| RC210013L3 | Lenti ORF clone of Human interleukin 2 (IL2), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210013L4 | Lenti ORF clone of Human interleukin 2 (IL2), mGFP tagged | 10 ug |
$450.00
|
|
| RG210013 | IL2 (tGFP-tagged) - Human interleukin 2 (IL2) | 10 ug |
$489.00
|
|
Citations
Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo
Ren, Liu, Ding et al
STAR Protocols (2022) 3 (1), 101217 DOI: 10.1016/j.xpro.2022.101217
Ren, Liu, Ding et al
STAR Protocols (2022) 3 (1), 101217 DOI: 10.1016/j.xpro.2022.101217
Cited by 18
AI Summary: OriGene products contributed to this study by providing the human IL-10 expression plasmid (Cat#: SC300099) and the human IL-2 expression plasmid (Cat#: SC125234), which were used for hydrodynamic injection to modulate human hematopoiesis in HIS mice.
Snippet: ration;12 weeks old HIS mice were used for hydrodynamic injection. Gempharmatech T001475 ------------------------- Recombinant DNA ------------------------- Human IL-10 expression plasmid Origene Cat#:SC300099 Human IL-2 expression plasmid Origene Cat#:SC125234 ------------------------- Software and algorithms ------------------------- NovoExpress Software Agilent Versi
Regulatory T cells control toxicity in a humanized model of IL-2 therapy
Li, Strick-Marchand, Lim et al
Nat Commun (2017) 8 (1), 1762 DOI: 10.1038/s41467-017-01570-9
Li, Strick-Marchand, Lim et al
Nat Commun (2017) 8 (1), 1762 DOI: 10.1038/s41467-017-01570-9
Cited by 60
AI Summary: OriGene's human IL-2 cDNA clone in the pCMV-6-XL4 vector was used to produce IL-2 for modeling immunotherapy toxicity in humanized mice in this study.
Snippet: A human IL-2 cDNA clone in the vector pCMV-6-XL4 (Origene) was purified using endotoxin-free Plasmid-Maxi kit (Cat ID: 12362, Qiagen)
MAC-T Cells as a Tool to Evaluate Lentiviral Vector Construction Targeting Recombinant Protein Expression in Milk
Monzani, Guemra, Adona et al
Anim. Biotechnol. (2015) 26 (2), 136-42 DOI: 10.1080/10495398.2014.941468
Monzani, Guemra, Adona et al
Anim. Biotechnol. (2015) 26 (2), 136-42 DOI: 10.1080/10495398.2014.941468
Cited by 9
A functional DC cross talk promotes human ILC homeostasis in humanized mice
Lopez-Lastra, Masse-Ranson, Fiquet et al
Blood Advances (2017) 1, 601
Lopez-Lastra, Masse-Ranson, Fiquet et al
Blood Advances (2017) 1, 601
Snippet: Hydrodynamic injection Full-length human IL-2, IL-7, IL-25, and IL-33 complementary DNA clones were cloned in the mammalian cell expression vector pCMV- 6-XL4 (Origene) and plasmids purified using the endotoxin-free Plasmid-Maxi kit (Qiagen);;Full-length human IL-2, IL-7, IL-25, and IL-33 complementary DNA clones were cloned in the mammalian cell expression vector pCMV-6-XL4 (Origene) and plasmids...
Showing 1-4 of 4 papers.
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