PPAR gamma (PPARG) (NM_005037) Human Untagged Clone

SKU SC124236

PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: PPAR gamma
• Synonyms: CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005037, the custom clone sequence may differ by one or more nucleotides; ; ; ATGACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTTGGGATCAGCTCCGTGGATCTCTCCG; TAATGGAAGACCACTCCCACTCCTTTGATATCAAGCCCTTCACTACTGTTGACTTCTCCAGCATTTCTAC; TCCACATTACGAAGACATTCCATTCACAAGAACAGATCCAGTGGTTGCAGATTACAAGTATGACCTGAAA; CTTCAAGAGTACCAAAGTGCAATCAAAGTGGAGCCTGCATCTCCACCTTATTATTCTGAGAAGACTCAGC; TCTACAATAAGCCTCATGAAGAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGTGTCTGTGGAGATAA; AGCTTCTGGATTTCACTATGGAGTTCATGCTTGTGAAGGATGCAAGGGTTTCTTCCGGAGAACAATCAGA; TTGAAGCTTATCTATGACAGATGTGATCTTAACTGTCGGATCCACAAAAAAAGTAGAAATAAATGTCAGT; ACTGTCGGTTTCAGAAATGCCTTGCAGTGGGGATGTCTCATAATGCCATCAGGTTTGGGCGGATGCCACA; GGCCGAGAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAATCCAGAGTCCGCTGAC; CTCCGGGCCCTGGCAAAACATTTGTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCAAAGGCGA; GGGCGATCTTGACAGGAAAGACAACAGACAAATCACCATTCGTTATCTATGACATGAATTCCTTAATGAT; GGGAGAAGATAAAATCAAGTTCAAACACATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGCCATCCGC; ATCTTTCAGGGCTGCCAGTTTCGCTCCGTGGAGGCTGTGCAGGAGATCACAGAGTATGCCAAAAGCATTC; CTGGTTTTGTAAATCTTGACTTGAACGACCAAGTAACTCTCCTCAAATATGGAGTCCACGAGATCATTTA; CACAATGCTGGCCTCCTTGATGAATAAAGATGGGGTTCTCATATCCGAGGGCCAAGGCTTCATGACAAGG; GAGTTTCTAAAGAGCCTGCGAAAGCCTTTTGGTGACTTTATGGAGCCCAAGTTTGAGTTTGCTGTGAAGT; TCAATGCACTGGAATTAGATGACAGCGACTTGGCAATATTTATTGCTGTCATTATTCTCAGTGGAGACCG; CCCAGGTTTGCTGAATGTGAAGCCCATTGAAGACATTCAAGACAACCTGCTACAAGCCCTGGAGCTCCAG; CTGAAGCTGAACCACCCTGAGTCCTCACAGCTGTTTGCCAAGCTGCTCCAGAAAATGACAGACCTCAGAC; AGATTGTCACGGAACACGTGCAGCTACTGCAGGTGATCAAGAAGACGGAGACAGACATGAGTCTTCACCC; GCTCCTGCAGGAGATCTACAAGGACTTGTACTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005037 unedited; NGGTGTTAGATTTGTAACCGACTACTATAGGCGGCCGCGCATTCGGCACCAGGGGGCCTT; AACCTCTGCTGGTGACCAGAAGCCTGCATTTCTGCATTCTGCTTAATTCCCTTTCCTTAG; ATTTGAAAGAAGCCAACACTAAACCACAAATATACAACAAGGCCATTTTCTCAAACGAGA; GTCAGCCTTTAACGAAATGACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTT; TGGGATCAGCTCCGTGGATCTCTCCGTAATGGAAGACCACTCCCACTCCTTTGATATCAA; GCCCTTCACTACTGTTGACTTCTCCAGCATTTCTACTCCACATTACGAAGACATTCCATT; CACAAGAACAGATCCAGTGGTTGCAGATTACAAGTATGACCTGAAACTTCAAGAGTACCA; AAGTGCAATCAAAGTGGAGCCTGCATCTCCACCTTATTATTCTGAGAAGACTCAGCTCTA; CAATAAGCCTCATGAAGAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGTGTCTGTGG; AGATAAAGCTTCTGGATTTCACTATGGAGTTCATGCTTGTGAAGGATGCAAGGGTTTCTT; CCGGAGAACAATCAGATTGAAGCTTATCTATGACAGATGTGATCTTAACTGTCGGATCCC; ACAAAAAGTAGAAATAAATGTCAGTACTGTCGGTTTCAGAAATGCCTTGCAGTGGGGATG; TCTCATAATGCCATCAGGTTTTGGCGGATGCCACANGCCGAGNAAGAGAAGCTGNNTGCN; GAGATCTNCAGTGATATCGNACAGCTGAATCCAGAGTCCGCTGACCTNCGGGCCCTGGCA; AAACATTGTATGACTCATACTAAAAAGTCTTTCCGCTGACCAAAGCAAAGCGAGGGCGAT; CTTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005037 unedited; AATTAGCTTGNCCGCGGCCGCAATCNANATCGAGTTTTTTTTTTTTTTTTTTAATATGGT; AATTTTTAATATTAAAAGTAAATTGTAAATGTGTCTTTATAAACAATATGCATAAAATAG; ATCATATTCTAAAACCTTTTCTTTTTAAAATGCTTTTTCACAGTAAATTTCTTAGGTGTC; AGATTTTCCCTCAGAATAGTGCAACTGGAAGAAGGGAAATGTTGGCAGTGGCTCAGGACT; CTCTGCTAGTACAAGTCCTTGTAGATCTCCTGCAGGAGCGGGTGAAGACTCATGTCTGTC; TCCGTCTTCTTGATCACCTGCAGTAGCTGCACGTGTTCCGTGACAATCTGTCTGAGGTCT; GTCATTTTCTGGAGCAGCTTGGCAAACAGCTGTGAGGACTCAGGGTGGTTCAGCTTCAGC; TGGAGCTCCAGGGCTTGTAGCAGGTTGTCTTGAATGTCTTCAATGGGCTTCACATTCAGC; AAACCTGGGCGGTCTCCACTGAGAATAATGACAGCAATAAATATTGCCAAGTCGCTGTCA; TCTAATTCCAGTGCATTGAACTTCACAGCAAACTCAAACTTGGGCTCCATAAAGTCACCA; AAAGGCTTTCGCAGGCTCTTTAGAAACTCCCTTGTCATGAAGCCCTTGGCCCTCGGATAT; GAGAACCCCATCTTTATTCATCAAGGAGGCCAGCATTGTGTAAATGATCTCGTGGACTCC; ATATTTGAGGAGAGTTACTTGGCCGTTCAGTCCAGATTTACAAAACCAGAATGCTTTTGG; CATACTCTGTGATCTCCTGCACAGCCCTTCAGGAACCGAAACTGGCAGCCCTGTAAGATG; CCGATGCCCCCTCTTTGCTCTGCTCTGCAGGGGGTGAAGGGTTTGAACTTGATTTATCTT; CTCCCATCATTAGGATTCATGTCTAGATACGATGGTGATTTGCCTGTGCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005037
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005037.4, NP_005028.3
• RefSeq Size: 1818 bp
• RefSeq ORF: 1818 bp
• Locus ID: 5468
• UniProt ID: P37231
• Cytogenetics: 3p25.2
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer
• Summary: This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region compared to variant 2. Variants 1, 3, 4, 6, and 7 all encode the same isoform (1), which has a shorter, distinct N-terminus compared to isoform 2.