LRRK2 (NM_198578) Human Untagged Clone
$3,129.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | LRRK2 |
| Synonyms | AURA17; DARDARIN; PARK8; RIPK7; ROCO2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_198578 |
| Insert Size | 9300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_198578.2, NP_940980.2 |
| RefSeq Size | 9234 bp |
| RefSeq ORF | 7584 bp |
| Locus ID | 120892 |
| UniProt ID | Q5S007 |
| Cytogenetics | 12q12 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Parkinson's disease |
| Summary | This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_198578" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216373 | LRRK2 (Myc-DDK-tagged)-Human leucine-rich repeat kinase 2 (LRRK2) | 10 ug |
$3,128.00
|
|
| RC216373L1 | Lenti ORF clone of Human leucine-rich repeat kinase 2 (LRRK2), Myc-DDK-tagged | 10 ug |
$3,428.00
|
|
| RG216373 | LRRK2 (tGFP-tagged) - Human leucine-rich repeat kinase 2 (LRRK2) | 10 ug |
$3,528.00
|
|
Citations
Genetic dissection reveals that Akt is the critical kinase downstream of LRRK2 to phosphorylate and inhibit FOXO1, and promotes neuron survival
Chuang, Lu, Wang et al
Hum. Mol. Genet. (2014)
Chuang, Lu, Wang et al
Hum. Mol. Genet. (2014)
Cited by 35
AI Summary: OriGene’s human wild-type LRRK2 cDNA was used in this study to generate expression constructs for investigating the role of LRRK2 in neuron survival and its downstream signaling through Akt and FOXO1.
Snippet: experiments were performed at least four times. Molecular cloning and transgene expression Human wild-type LRRK2 cDNA (Origene Technologies, Rockville, MD) was subcloned into a pUAST vector and was confirmed by DNA sequencing. We obtained multiple ... Cite SaveSaving
Evidence that the LRRK2 ROC domain Parkinson's disease-associated mutants A1442P and R1441C exhibit increased intracellular degradation
Greene, Mastaglia, Meloni et al
J. Neurosci. Res. (2013)
Greene, Mastaglia, Meloni et al
J. Neurosci. Res. (2013)
Cited by 22
Showing 1-2 of 2 papers.
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