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LRRC56 (NM_198075) Human Untagged Clone

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SKU
SC123392
LRRC56 (untagged)-Human leucine rich repeat containing 56 (LRRC56)
  $505.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol LRRC56
Synonyms CILD39
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_198075 edited
GGAGTAGGAGACAGCGCCTGGAGGTGGAGGGCGCCCAGGGCCGAGCTGCCAGGGCCGGAC
ACCTAGGCTGAGCCCTCAGCCGGCCAACTCGCCTGGCACAGGGGCAGCTCTAGACGGGAT
GGAGAGGATGCATCTTCAGTGTCCACACGTGGTCAACAGATAAAAGCCATGGCCAGAACG
TTCTAAGTGGCTACATGTGCATCTGCCTGTTCACGGATTCCTTCGACAAATATTTGAGCA
TCCCCTCCTGCGACTGTGGACAGCAGAGGAGCACGCAGGCCACGTGCAGCCCACAGCTCT
GGGGCGAGGCAGGCCCTCAACCGAACACGTATAGAGACAGGGTTTCACCGTGTTAGCCAG
GATGGTCTCAATCTCCTGACCTCGTGATCTGCCCGCCTCAGCCTCCCAAAGTGCTGGGAT
TACAGGCGTGAGCCACTGCGCCCCGCCAGACGTCTGCATCTTTAAGGGGCAACACCAGGT
GACATGTGAATGGATCTGGGCTGGGACAGATCCCGTGGGCCTCGGCGGAGCACCTCCAGC
GTCCGGGTGCGGGAGCTGAGCTGGCAAGGCCTGCACAACCCCTGCCCACAGAGCAAGGGC
CCTGGCAGTCAGAGGGACAGACTTGGAGAGCAGCTGGTGGAAGAGTACCTGTCCCCTGCC
CGGCTGCAGGCCCTGGCCCGGGTGGATGACCTTCGGCTGGTGAGGACGCTGGAGATGTGT
GTGGACACTCGTGAGGGCAGCCTGGGGAACTTTGGGGTGCACCTGCCCAACCTGGACCAA
CTGAAGCTGAACGGCAGCCACCTGGGCTCCCTGAGGGACTTGGGCACGTCTCTGGGCCAC
CTGCAGGTGCTGTGGCTGGCTCGCTGTGGCCTCGCTGACCTGGATGGCATCGCCTCTTTG
CCAGCACTTAAGGAACTCTACGCCTCCTACAACAACATCTCGGACCTGAGCCCACTGTGC
CTGCTGGAACAATTGGAGGTGCTGGACCTGGAGGGCAACAGCGTGGAGGACCTGGGGCAG
GTGCGCTACTTGCAGCTGTGCCCACGCCTGGCCATGCTCACCCTGGAGGGCAACCTGGTG
TGCCTACAGCCGGCCCCTGGCCCCACCAACAAGGTGCCCAGGGGCTACAACTACAGGGCA
GAGGTGAGGAAGCTCATTCCCCAGCTGCAGGTCCTGGACGAAGTGCCGGCCGCACACACA
GGCCCACCGGCCCCCCCGCGGCTGAGCCAGGACTGGCTTGCGGTGAAGGAGGCCATCAAG
AAGGGCAACGGCCTTCCCCCGCTGGACTGTCCCCGTGGAGCCCCCATCCGGAGACTTGAC
CCCGAGCTGTCCCTGCCTGAGACGCAGTCCCGGGCCTCCAGGCCCTGGCCCTTCTCCCTG
CTGGTCCGTGGGGGCCCCCTGCCTGAAGGCCTGCTTTCTGAGGACCTGGCCCCAGAAGAT
AACCCAGCAGCCTCACCCATGGTGCTGGCCAAGTCCTCTGTGGGAACCCCACCAAGGGCC
TGCGGGAGCGTAGGCACCAGTGCCAGGCCAGGGAGCCCCCCGAGCAGCTGCCCCAACACA
GGCCAGGAGATCCGGCCGCCAGCACTTCCACCCCAGAGCCTGACCCTGCAGACAGCTCTG
ACTTCCTGGCCTTGGCTGGGCTCAGGGCCTGGAGGGAACATGGCGTGCGCCCCCTCCCCT
ATAGGCACCCGGAGTCCCAACAGGAAGGGGCTGTAGCCCCCTGGGGCCCACGGAGGGTCC
CTGAAGAGCAAGTGCACCAGGCAGAGCCCAAGACTCCCTCCAGCCCCCCAAGCCTGGCCT
CAGAGCCCTCCGGGACCTCGAGCCAGCACCTGGTCCCTTCACCTCCCAAGCACCCAAGGC
CACGAGATTCTGGCAGCAGCTCCCCGCGGTGGTCGACAGACCTGCAGTCCAGGGGGCGTC
GGCTCCGAGTCCTGGGCAGCTGGGGGCCTGGCCTGGGTGATGGGGTGGCTGCAGTGCCTG
TCCTGAGAGCCCTGGAGGTGGCCTCACGCCTGAGCCCTCGAGCCCAGGGATGTCCTGGCC
CAAAGCCAGCACAAGATGCAGCAGCTAGACCTCCCAGGGCAGCTGAACTCTCTCACCCCA
GCCCCGTCCCCACTTAATATAGCCCCCACTGCCAGGCTTCCCTGTGCTGGGGCCACGACT
TGCCCACATATGTGGTCACAGAGCACAGAATACCAGGGCGGGTGTGTTGGGGGGTGGAAG
GAGTGCCTGGCCCTGGGGAGGACCCTCTTGGTGGAGGGGAGTGGGGGACTGGGACCAGCC
AGGGAGGCAGCAGAGGCTGGAACCCAGTTTAGGCCCCCAACTGGGTTTGGCCTGGGGAGG
GAGGGTCCGGCTCCCCAGCCATTCCTTAGGGCCAGGCTTTCCCGCGGGCACGGGGGTGGG
GGGTGGTCACCCGAGCAGGCCTGTGAGAGGCCTCTCCTGCTAGAATTGGGCATGGCCGAG
GGGCAGGGGCTGGAGCTGCGAGTCCACCACTCCCTTGCCGGCCCCAGGGTAAGAGCCACC
TCCTAGGCCAGTGGCCCAAGGTCCCAGCTGCTCGCCTGAGGCCGCCGGGGGTGCGGTCCA
GGCCTCCCGTCTCCGGGGGATCTGTAGGGTTCCCGCACTGCGATAGGGCGGCCCGTTCCC
TCCTCTTGGCGCAGGACGCCCCGGAACCCAAACCAACATTTCCAGCTCTCAGGTGTACAG
AAATGCGGTTTACTTTGTAGGCCACGTTGGTTCAATAAATGATGCAGCGGACACAAAAAA
AAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_198075 unedited
GACTGNGTTTTGTAAACGACTTTTATGGGCGGCCGCATAACTTCGTATAGCATACATTAT
ACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGGAGTAGG
AGACAGCGCCTGGAGGTGGAGGGCGCCCAGGGCCGAGCTGCCAGGGCCGGACACCTAGGC
TGAGCCCTCAGCCGGCCAACTCGCCTGGCACAGGGGCAGCTCTAGACGGGATGGAGAGGA
TGCATCTTCAGTGTCCACACGTGGTCAACAGATAAAAGCCATGGCCAGAACGTTCTAAGT
GGCTACATGTGCATCTGCCTGTTCACGGATTCCTTCGACAAATATTTGAGCATCCCCTCC
TGCGACTGTGGACAGCAGAGGAGCACGCAGGCCACGTGCAGCCCACAGCTCTGGGGCGAG
GCAGGCCCTCAACCGAACACGTATAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGTCT
CAATCTCCTGACCTCGTGATCTGCCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCG
TGAGCCACTGCGCCCCGCCAGACGTCTGCATCTTTAAGGGGCAACACCAGGTGACATGTG
AATGGATCTGGGCTGGGACAGATCCCGTGGGCCTCGGCGGAGCACCTCCAGCGTCCGGGT
GCGGGAGCTGAGCTGGCAAGGCCTGCACAACCCCTGCCCACAGAGCAAGGGCCCTGGCAG
TCAGAGGGACAGACTTGGAGAGCAGCTGGTGGAAGAGTACCTGTCCCCTGCCCGGCTGCA
GGCCCTGGCCCCGGGTGGATGACCTTNNCGCTGGTGAGGACGCTGGAGATGTGTGTGGAC
ACTCGTGAGGGCAGCCTGGGGGACTTTGGGTGCACCTGCCAACCTGGACCAACTGAGCTG
AACGCACCACCTGGGCTCCCTGAGGGACTTGGCACGTCTCT
Restriction Sites Please inquire |
ACCN NM_198075
Insert Size 2770 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198075.1, NP_932341.1
RefSeq Size 2777 bp
RefSeq ORF 1629 bp
Locus ID 115399
UniProt ID Q8IYG6
Cytogenetics 11p15.5
Summary Required for the assembly of dynein arms.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198075" in other vectors (4)
SKU Description Size Price
RC205170 LRRC56 (Myc-DDK-tagged)-Human leucine rich repeat containing 56 (LRRC56) 10 ug
$505.00
RC205170L3 Lenti ORF clone of Human leucine rich repeat containing 56 (LRRC56), Myc-DDK-tagged 10 ug
$805.00
RC205170L4 Lenti ORF clone of Human leucine rich repeat containing 56 (LRRC56), mGFP tagged 10 ug
$805.00
RG205170 LRRC56 (tGFP-tagged) - Human leucine rich repeat containing 56 (LRRC56) 10 ug
$705.00

file-alt Citations

Biallelic Mutations in LRRC56, Encoding a Protein Associated with Intraflagellar Transport, Cause Mucociliary Clearance and Laterality Defects
Bonnefoy, Watson, Kernohan et al
Am J Hum Genet (2018) 103 (5), 727-739 DOI: 10.1016/j.ajhg.2018.10.003
Cited by 73
AI Summary: In this study, OriGene’s human LRRC56 Myc-DDK-tagged cDNA clone (RC217684) was used to investigate the function and localization of LRRC56, contributing to the characterization of its role in cilia assembly and motility defects associated with biallelic LRRC56 mutations.
Biallelic Mutations in LRRC56 encoding a protein associated with intraflagellar transport, cause mucociliary clearance and laterality defects
Bonnefoy, Watson, Kernohan et al
bioRxiv (2018)
Snippet: 142 143 Co-IP experiments 144 Human LRRC56 was amplified from an untagged image clone (SC123392, Origene 145 Technologies), inserted into the pDONR201 Gateway cloning vector (Invitrogen) and 146 not peer-reviewed) is the author/funder. All rights reserved View as HTML
Showing 1-2 of 2 papers.
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