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ASCC1 (BC012291) Human Untagged Clone

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SKU
SC122322
ASCC1 (untagged)-Human activating signal cointegrator 1 complex subunit 1 (cDNA clone MGC:8735 IMAGE:3858865), complete cds
  $2,024.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ASCC1
Synonyms activating signal cointegrator 1 complex subunit 1; ASC-1 complex subunit P50; ASC1p50; CGI-18; OTTHUMP00000019781; OTTHUMP00000019782; OTTHUMP00000019783; RP11-150D20.4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for BC012291 edited
AGAGAACGCGCTCGCGTAGAGGAATTCCGGGTCCTCCTCCTCGCCCTACCTTGGTTTACC
TGCAGCCGCCTAGTCCTCTTCTCCTTCTCTTTGATGATTCAGGGCCTCGGCTTCCCTCAC
GGGCATAATTGGAATATCATTTGGAGAAAGTGTCATGGAAGTTCTGCGTCCACAGCTTAT
AAGAATTGATGGCCGGAATTACAGGAAGAATCCAGTCCAAGAACAGACCTATCAACATGA
AGAAGATGAAGAGGACTTCTATCAAGGCTCCATGGAGTGTGCTGATGAGCCCTGTGATGC
CTACGAGGTGGAGCAGACCCCACAAGGATTCCGGTCTACTTTGAGGGCCCCCAGCTTGCT
CTATAAGCATATAGTTGGAAAGAGAGGGGACACTAGGAAGAAAATAGAAATGGAGACCAA
AACTTCTATTAGCATTCCTAAACCTGGACAAGACGGGGAAATTGTAATCACTGGCCAGCA
TCGAAATGGTGTAATTTCAGCCCGAACACGGATTGATGTTCTTTTGGACACTTTTCGAAG
AAAGCAGCCCTTCACTCACTTCCTTGCCTTTTTCCTCAATGAAGTTGAGGTTCAGGAAGG
ATTCCTGAGATTCCAGGAGGAAGTACTGGCGAAGTGCTCCATGGATCATGGGGTTGACAG
CAGCATTTTCCAGAATCCTAAAAAGCTTCATCTAACTATTGGGATGTTGGTGCTTTTGAG
TGAGGAAGAGATCCAGCAGACATGTGAGATGCTACAGCAGTGTAAAGAGGAATTCATTAA
TGATATTTCTGGGGGTAAACCCCTAGAAGTGGAGATGGCAGGGATAGAATACATGAATGA
TGATCCTGGCATGGTGGATGTTCTTTACGCCAAAGTCCATATGAAAGATGGCTCCAACAG
GCTACAAGAATTAGTTGATCGAGTGCTGGAACGTTTTCAGGCATCTGGACTAATAGTGAA
AGAGTGGAATAGTGTGAAACTGCATGCTACAGTTATGAATACACTATTCAGGAAAGACCC
CAATGCTGAAGGCAGGTACAATCTCTACACAGCGGAAGGCAAATATATCTTCAAGGAAAG
AGAATCATTTGATGGCCGAAATATTTTAAAGTTGTTTGAGAACTTCTACTTTGGCTCCCT
AAAGCTGAATTCAATTCACATCTCTCAGAGGTTCACCGTAGACAGCTTTGGAAACTACGC
TTCCTGTGGACAAATTGACTTCTCCTGAGGTGGATCTTGGAAAGCACTAGAAACTAAACA
TCTTCACCAGGTGCTGAAGAAAAGTGTCTTCGTTTTAATTGCCAAGCAGGGATGTGGACA
TTTGGATGGTGACTTCCCTGGGTGGTTCCCCATAGATTCACCATTGCCTCTAATGGTGTC
TACACCCGTCATACTACCAGCTGAGATGGTGGTGGGCATAAGGAGAATTTGTGCCTATAA
CCCTTAGTGTGTTCTGGTTTTTTTTCTTTTAATTTTTAAATTGTCGTAAAATACTCATAA
AACATACTGTCTTCACCATTTTTAAGTGCACAGTTCAGTAACGTTAACTGTTAATACATT
CATAATGCTGTGTGGCCGTCACCGCCGTCCATCTCCATAGGCTTCTCAGCTTGTAAAATG
GAAACTGTACCCATTAAACAGTAATTCCCACTCCTCCCAGAAAAAAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for BC012291 unedited
NGTTGGATTTGTATACGACTCATATGGGCGGCCGCGCATTCANATCTGGTACCGGTCCGG
AATTCCCGGGATATCGTCGACCCACGCGTCCGAGAGAACGCGCTCGCGTAGAGGAATTCC
GGGTCCTCCTCCTCGCCCTACCTTGGTTTACCTGCAGCCGCCTAGTCCTCTTCTCCTTCT
CTTTGATGATTCAGGGCCTCGGCTTCCCTCACGGGCATAATTGGAATATCATTTGGAGAA
AGTGTCATGGAAGTTCTGCGTCCACAGCTTATAAGAATTGATGGCCGGAATTACAGGAAG
AATCCAGTCCAAGAACAGACCTATCAACATGAAGAAGATGAAGAGGACTTCTATCAAGGC
TCCATGGAGTGTGCTGATGAGCCCTGTGATGCCTACGAGGTGGAGCAGACCCCACAAGGA
TTCCGGTCTACTTTGAGGGCCCCCAGCTTGCTCTATAAGCATATAGTTGGAAAGAGAGGG
GACACTAGGAAGAAAATAGAAATGGAGACCAAAACTTCTATTAGCATTCCTAAACCTGGA
CAAGACGGGGAAATTGTAATCACTGGCCAGCATCGAAATGGTGTAATTTCAGCCCGAACA
CGGATTGATGTTCTTTTGGACACTTTTCGAAGAAAGCAGCCCTTCACTCACTTCCTTGCC
TTTTTCCTCAATGAAGTTGNAGGTTCAGGAAGATTCCTGAGATTCCAGGAGGAAGTACTG
GCGAAGTGCTCCATGGATCATGGGGTTGACAGCAGCATTTTCCAGAATCCTANAAGCTTC
ATCTAACTATTGGGATGNTNNGTGCTTTGAGTGANGGAAGAGATCCAGCAGACATGTGAG
ATGCTACAGCAGTGTAAAGAAGGAATTCATTAATGANNATTTCTGGGGGGGTAACCCCCT
ANAAGTGGAGATGGCAGGNATAGAAA
Restriction Sites Please inquire |
ACCN BC012291
Insert Size 1678 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC012291.1, AAH12291.1
RefSeq Size 1678 bp
Locus ID 51008
Cytogenetics 10q22.1
Summary This gene encodes a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB) and serum response factor (SRF). The encoded protein contains an N-terminal KH-type RNA-binding motif which is required for AP-1 transactivation by the ASC-1 complex. Mutations in this gene are associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. provided by RefSeq, Dec 2011
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