KCNK10 (NM_138317) Human Untagged Clone
SKU
SC121923
KCNK10 (untagged)-Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2
$506.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | KCNK10 |
| Synonyms | K2p10.1; PPP1R97; TREK-2; TREK2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_138317 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_138317.1, NP_612190.1 |
| RefSeq Size | 2484 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 54207 |
| UniProt ID | P57789 |
| Cytogenetics | 14q31.3 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Summary | The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. provided by RefSeq, Sep 2008 Transcript Variant: This variant (2, also known as TREK2 splice variant c) has a different 5' terminal exon compared to transcript variant 1, and encodes a slightly longer isoform (2) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Reviews
Documents
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| FAQs |
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Resources
"NM_138317" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223229 | KCNK10 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2 | 10 ug |
$505.00
|
|
| RC223229L1 | Lenti ORF clone of Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2, Myc-DDK-tagged | 10 ug |
$805.00
|
|
| RC223229L2 | Lenti ORF clone of Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2, mGFP tagged | 10 ug |
$805.00
|
|
| RC223229L3 | Lenti ORF clone of Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2, Myc-DDK-tagged | 10 ug |
$805.00
|
|
| RC223229L4 | Lenti ORF clone of Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2, mGFP tagged | 10 ug |
$805.00
|
|
| RG223229 | KCNK10 (tGFP-tagged) - Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2 | 10 ug |
$489.00
$705.00
|
|
Citations
Block of TREK and TRESK K2P channels by lamotrigine and two derivatives sipatrigine and CEN-092
Walsh, Leach, Veale et al
Biochem Biophys Rep (2021) 26, 101021 DOI: 10.1016/j.bbrep.2021.101021
Walsh, Leach, Veale et al
Biochem Biophys Rep (2021) 26, 101021 DOI: 10.1016/j.bbrep.2021.101021
Cited by 5
AI Summary: OriGene’s PCMV6-XL-4 plasmid containing human TREK-1 and TREK-2 genes was used to express these potassium channels in cells, enabling the study of how lamotrigine and its derivatives inhibit channel activity.
Snippet: given below. 2.1 Mammalian expression plasmids Human TREK-1 (KCNK2, Genbank NP_055032.1) and TREK2 (KCNK10, NP_612190.1) was cloned into pcDNA3.1+ (Invitrogen, Carlsbad, CA, USA) and PCMV6-XL-4 (OriGene Technologies, Inc. USA). Human TRESK (KCNK18, NP_862823.1) was cloned into pFAW-Ac-GFP vector and was a kind gift from Dr Eddie Stevens, Pfizer, UK. 2.2 Mutations Point mutations were introdu
Showing 1-1 of 1 papers.
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