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ADHFE1 (NM_144650) Human Untagged Clone

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SKU
SC120771
ADHFE1 (untagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein
  $686.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ADHFE1
Synonyms ADH8; HMFT2263; HOT
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_144650 edited
GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG
AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCTGCCGCCCGAG
CCCGGGTCGCGTACTTGCTGAGGCAACTGCAACGCGCAGCCCCCTGGACTTTCACCTTCT
GGGAAAACAACAGATTATGCCTTTGAGATGGCTGTTTCAAATATTAGATATGGAGCAGCA
GTTACAAAGGAAGTAGGAATGGACCTAAAAAACATGGGTGCTAAAAATGTGTGCTTGATG
ACAGACAAGAACCTCTCCAAGCTCCCTCCTGTGCAAGTAGCTATGGATTCCCTAGTGAAG
AATGGCATCCCCTTTACGGTTTATGATAATGTGAGAGTGGAACCAACGGATTCAAGCTTC
ATGGAAGCTATTGAGTTTGCCCAAAAGGGAGCTTTTGATGCCTATGTTGCTGTCGGTGGT
GGCTCTACCATGGACACCTGTAAGGCTGCTAATCTGTATGCATCCAGCCCTCATTCTGAT
TTCCTAGATTATGTCAGTGCCCCCATTGGCAAGGGAAAGCCTGTGTCTGTGCCTCTTAAG
CCTCTGATTGCAGTGCCAACTACCTCAGGAACCGGGAGTGAAACTACTGGGGTTGCCATT
TTTGACTATGAACACTTGAAAGTAAAAATTGGCATCACTTCGAGAGCCATCAAACCCACA
CTGGGACTGATTGATCCTCTGCACACCCTCCACATGCCTGCCCGAGTGGTCGCCAACAGT
GGCTTTGATGTGCTTTGCCATGCCCTGGAGTCATACACCACCCTGCCCTACCACCTGCGG
AGCCCCTGCCCTTCAAATCCCATCACACGGCCTGCGTACCAGGGCAGCAACCCAATCAGT
GACATTTGGGCTATCCACGCGCTGCGGATCGTGGCTAAGTATCTGAAGAGGGCCGTCAAC
AGCACTGACAAATGAAGAAGAAGAATGAGTCTCAGGGCACCTTCCTGGAGTGGCCCTCGC
CATCCAGGGACCAGCGCGTTGCCTCCCCCGGCGCTTTTACATCACATGAGCAGCTGACAG
CGTATTTATTATGCCTTTGCTGAAACAGTTGATGAGAAATATGTTTCCCTTCAGTTTTCA
TTTAGAAGACAAGGCGTCGTTTGAATAACTTGCCTGATTTTTCTTCCTACCGAGTAGCAT
TTCTTTTTTTTTCACCATATCTTTCCCTAAGGCAGCTCCTTATTCTGTAGGAATTGCCAA
TGTTGATGTGTTATTGTTAGGTATTTATATCATTCACAGGGCTGTCAGAAATCCCGATGA
TCTTGAAGCAAGGTCTCATATGCACTTGGCAAGTGCTTTTGCTGGCATCGGCTTTGGAAA
TGCTGGTGTTCATCTGTGCCATGGAATGTCTTACCCAATTTCAGGTTTAGTGAAGATGTA
TAAAGCAAAGGATTACAATGTGGATCACCCACTGGTGCCCCATGGCCTTTCTGTGGTGCT
CACGTCCCCAGCGGTGTTCACTTTCACGGCCCAGATGTTTCCAGAGCGACACCTGGAGAT
GGCAGAAATACTGGGAGCCGACACCCGCACTGCCAGGATCCAAGATGCAGGGCTGGTGTT
GGCAGACACGCTCCGGAAATTCTTATTCGATCTGGATGTTGATGATGGCCTAGCAGCTGT
TGGTTACTCCAAAGCTGATATCCCCGCACTAGTGAAAGGAACGCTGCCCCAGGAAAGGGT
CACCAAGCTTGCACCCCGTCCCCAGTCAGAAGAGGATCTGGCTGCTCTGTTTGAAGCTTC
AATGAAACTGTATTAATTGTCATTTTAACTGAAAGAATTACCGCTGGCCATTGTAGTGCT
GAGAGCAAGAGCTGATCTAGCTAGGGCTTTGTCTTTTCATCTTTGTGCATAACTTACCTG
TTACCAGTATAGGTGGGATATACATTTATCTTGCAGGAAATTCCCCAAAGCTCAGAGTCC
AGTTCCTTCCATAAAACAGGCTGGACAAATGACCACTATGTTAGACCCCCAGGCTCGACT
TCAGGGGTCAGTGTTCCTGTCCCAAACCCCACACAGAATACTCTGCCTCTGCTTCATGTA
GCAAATGAGCAAAAACTCAGTATCTATCAAAAGTGTAAATTATATTTCCTATGCCTAGTA
ATTCACTTCATGTCTAAAAATTTATCTGATAGAAACACTAGCACCAGTACATACAGAAGC
ATGGCAAGGATGTTTCTGGCAGCACTTTTCTAATAATAAAAGATTTGAAACAACAAAAAA
AAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_144650 unedited
CCAACCGGCACACCCCCACCCTCCCCCCCCCCCGTTCAGATTTGNATACAACTCATATAG
GCGGCCGCGAAATTCGCACCAGCTGCCGCCCGAGCCCGGGTCGCGTACTTGCTGAGGCAA
CTGCAACGCGCAGCCCCCTGGACTTTNNCACCTTCTGGGAAAACAACAGATTATGCCTTT
GAGATGGCTGTTTCAAATATTAGATATGGAGCAGCAGTTACAAAGGAAGTAGGAATGGAC
CTAAAAAACATGGGTGCTAAAAATGTGTGCTTGATGACAGACAAGAACCTCTCCAAGCTC
CCTCCTGTGCAAGTAGCTATGGATTCCCTAGTGAAGAATGGCATCCCCTTTACGGTTTAT
GATAATGTGAGAGTGGAACCAACGGATTCAAGCTTCATGGAAGCTATTGAGTTTGCCCAA
AAGGGAGCTTTTGATGCCTATGTTGCTGTCGGTGGTGGCTCTACCATGGACACCTGTAAG
GCTGCTAATCTGTATGCATCCAGCCCTCATTCTGATTTCCTAGATTATGTCAGTGCCCCC
ATTGGCAAGGGAAAGCCTGTGTCTGTGCCTCTTAAGCCTCTGATTGCAGTGCCAACTACC
TCANGAACCGGGAGTGAAACTACTGNGGTTGCCATTTTTGACTATGAACACTTGAAAGTA
AAAATTGGCATCACTTTCGAGAGCCATCAAACCCACACTGGGACTGATTGATCCTCTGCA
CACCCTTCACATGCCTGCCCGAGTGGTCGCCACAGTGGCTTTGATGTGCTTTGCATGCCC
TGNAGTCATACACCACCCTTGCCTAACCACCTGCGGAGCCCTGCCCTTCAAATNCCATTA
CACGGCCTGCGTACCNGGGCAGCAACCCAATCANTGACATTTGGGCTATTCACGCCCTGC
GGATCGGGCCTAAATTTCTGAGAGGGCCGTCAACAGCCTGGCAAATGAAGAGAAAAAGGA
CCTTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_144650 unedited
GACTCACTATGGTCGTGGCCGCCACCTAAGATCGGTTTTTTTTTTTTTTTTTTGTTGTTT
CAAATCTTTTATTATTAGAAAAGTGCTGCCAGAAACATCCTTGCCATGCTTCTGTATGTA
CTGGTGCTAGTGTTTCTATCAGATAAATTTTTAGACATGAAGTGAATTACTAGGCATAGG
AAATATAATTTACACTTTTGATAGATACTGAGTTTTTGCTCATTTGCTACATGAAGCAGA
GGCAGAGTATTCTGTGTGGGGTTTGGGACAGGAACACTGACCCCTGAAGTCGAGCCTGGG
GGTCTAACATAGTGGTCATTTGTCCAGCCTGTTTTATGGAAGGAACTGGACTCTGAGCTT
TGGGGAATTTCCTGCAAGATAAATGTATATCCCACCTATACTGGTAACAGGTAAGTTATG
CACAAAGATGAAAAGACAAAGCCCTAGCTAGATCAGCTCTTGCTCTCAGCACTACAATGG
CCAGCGGTAATTCTTTCAGTTAAAATGACAATTAATACAGTTTCATTGAAGCTTCAAACA
GAGCAGCCAGATCCTCTTCTGACTGGGGACGGGGTGCAAGCTTGGTGACCCTTTCCTGGG
GCAGCGTTCCTTTCACTAGTGCGGGGATATCAGCTTTGGAGTAACCAACAGCTGCTAGGC
CATCATCAACATCCAGATCGAATAAGAATTTCCGGAGCGTGTCTGCCAACACCAGCCCTG
CATCTTGGATCCTGGCAGTGCGGGTGTCGGCTCCCAGTATTTCTGCCATCTCCAGGTGTC
GCTCTGGAAACATCTGGGCCGTGAAAGTGAACACCGCTGGGGGACGTGAGCACCACAGAT
AGGCCATGGGGCACCAGTGGGTGATCCACATTGTNATCCTTTGGCTTATACATCTTG
Restriction Sites NotI-NotI |
ACCN NM_144650
Insert Size 2250 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144650.1, NP_653251.1
RefSeq Size 1830 bp
RefSeq ORF 1260 bp
Locus ID 137872
UniProt ID Q8IWW8
Cytogenetics 8q13.1
Domains Fe-ADH
Summary The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 PubMed 16616524).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_144650" in other vectors (5)
SKU Description Size Price
RC223298 ADHFE1 (Myc-DDK-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein 10 ug
$686.00
RC223298L3 Lenti-ORF clone of ADHFE1 (Myc-DDK-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein 10 ug
$986.00
RC223298L4 Lenti-ORF clone of ADHFE1 (mGFP-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein 10 ug
$986.00
RG223298 ADHFE1 (tGFP-tagged) - Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein 10 ug
$886.00
SC317769 ADHFE1 (untagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein 10 ug
$686.00

file-alt Citations

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