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GBA (NM_000157) Human Untagged Clone

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SKU
SC120080
GBA (untagged)-Human glucosidase, beta, acid (GBA), transcript variant 1
  $500.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GBA
Synonyms GBA1; GCB; GLUC
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC120080 sequence for NM_000157 edited (data generated by NextGen Sequencing)
ATGGAGTTTTCAAGTCCTTCCAGAGAGGAATGTCCCAAGCCTTTGAGTAGGGTAAGCATC
ATGGCTGGCAGCCTCACAGGATTGCTTCTACTTCAGGCAGTGTCGTGGGCATCAGGTGCC
CGCCCCTGCATCCCTAAAAGCTTCGGCTACAGCTCGGTGGTGTGTGTCTGCAATGCCACA
TACTGTGACTCCTTTGACCCCCCGACCTTTCCTGCCCTTGGTACCTTCAGCCGCTATGAG
AGTACACGCAGTGGGCGACGGATGGAGCTGAGTATGGGGCCCATCCAGGCTAATCACACG
GGCACAGGCCTGCTACTGACCCTGCAGCCAGAACAGAAGTTCCAGAAAGTGAAGGGATTT
GGAGGGGCCATGACAGATGCTGCTGCTCTCAACATCCTTGCCCTGTCACCCCCTGCCCAA
AATTTGCTACTTAAATCGTACTTCTCTGAAGAAGGAATCGGATATAACATCATCCGGGTA
CCCATGGCCAGCTGTGACTTCTCCATCCGCACCTACACCTATGCAGACACCCCTGATGAT
TTCCAGTTGCACAACTTCAGCCTCCCAGAGGAAGATACCAAGCTCAAGATACCCCTGATT
CACCGAGCCCTGCAGTTGGCCCAGCGTCCCGTTTCACTCCTTGCCAGCCCCTGGACATCA
CCCACTTGGCTCAAGACCAATGGAGCGGTGAATGGGAAGGGGTCACTCAAGGGACAGCCC
GGAGACATCTACCACCAGACCTGGGCCAGATACTTTGTGAAGTTCCTGGATGCCTATGCT
GAGCACAAGTTACAGTTCTGGGCAGTGACAGCTGAAAATGAGCCTTCTGCTGGGCTGTTG
AGTGGATACCCCTTCCAGTGCCTGGGCTTCACCCCTGAACATCAGCGAGACTTCATTGCC
CGTGACCTAGGTCCTACCCTCGCCAACAGTACTCACCACAATGTCCGCCTACTCATGCTG
GATGACCAACGCTTGCTGCTGCCCCACTGGGCAAAGGTGGTACTGACAGACCCAGAAGCA
GCTAAATATGTTCATGGCATTGCTGTACATTGGTACCTGGACTTTCTGGCTCCAGCCAAA
GCCACCCTAGGGGAGACACACCGCCTGTTCCCCAACACCATGCTCTTTGCCTCAGAGGCC
TGTGTGGGCTCCAAGTTCTGGGAGCAGAGTGTGCGGCTAGGCTCCTGGGATCGAGGGATG
CAGTACAGCCACAGCATCATCACGAACCTCCTGTACCATGTGGTCGGCTGGACCGACTGG
AACCTTGCCCTGAACCCCGAAGGAGGACCCAATTGGGTGCGTAACTTTGTCGACAGTCCC
ATCATTGTAGACATCACCAAGGACACGTTTTACAAACAGCCCATGTTCTACCACCTTGGC
CACTTCAGCAAGTTCATTCCTGAGGGCTCCCAGAGAGTGGGGCTGGTTGCCAGTCAGAAG
AACGACCTGGACGCAGTGGCACTGATGCATCCCGATGGCTCTGCTGTTGTGGTCGTGCTA
AACCGCTCCTCTAAGGATGTGCCTCTTACCATCAAGGATCCTGCTGTGGGCTTCCTGGAG
ACAATCTCACCTGGCTACTCCATTCACACCTACCTGTGGCGTCGCCAGTGA

Clone variation with respect to NM_000157.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000157 unedited
AACATTTTGTAATACGACTTACTATAGGGCGCTCNCGCGAATTCGCACGAGGCTAGTGCC
TATAGCTAAGGCAGGTACCTGCATCCTTGTTTTTGTTTAGTGGATCCTCTATCCTTCAGA
GACTCTGGAACCCCTGTGGTCTTCTCTTCATCTAATGACCCTGAGGGGATGGAGTTTTCA
AGTCCTTCCAGAGAGGAATGTCCCAAGCCTTTGAGTAGGGTAAGCATCATGGCTGGCAGC
CTCACAGGATTGCTTCTACTTCAGGCAGTGTCGTGGGCATCAGGTGCCCGCCCCTGCATC
CCTAAAAGCTTCGGCTACAGCTCGGTGGTGTGTGTCTGCAATGCCACATACTGTGACTCC
TTTGACCCCCCGACCTTTCCTGCCCTTGGTACCTTCAGCCGCTATGAGAGTACACGCAGT
GGGCGACGGATGGAGCTGAGTATGGGGCCCATCCAGGCTAATCACACGGGCACAGGCCTG
CTACTGACCCTGCAGCCAGAACAGAAGTTCCAGAAAGTGAAGGGATTTGGAGGGGCCATG
ACAGATGCTGCTGCTCTCAACATCCTTGCCCTGTCACCCCCTGCCCAAAATTTGCTACTT
AAATCGTACTTCTCTGAAGAAGGAATCGGATATAACATCATCCGGGTACCCATGGCCAGC
TGTGACTTCTCCATCCGCACCTACACCTATGCAGACACCCCTGATGATTTCCAGTTGCAC
AACTTCAGCCTCCCAGAGGAAGATACCAAGCTCAAGATACCCCTGATTCACCGAGCCCTG
CANGTGGCCCAGCGTCCCCGTTCACTCCTTGCCAGCCCCTGGACATCACCCACTTGGNCT
CAAGACCATGGAGCGGTGAATGGGAAGGGGTCACTNCAGGGACAGNCCGNAGACATCTAC
ACCAGACCTGNGCCAGAACTTTGTGAAGTCCTGGATGCTATGCTGAGCCAGTTACANGTC
TGGGCN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000157 unedited
AACCTAGCTCAACTTTTCTCTTTAANACTGCCTTTTTCCTGGCTGGTGCCATAGGAATCA
TCTGGGTGCCTGGGCACACCCGCTGCTGCTTTAAGGCTTCCGCCCTGATGCTGACACTGC
TGCTCCACGGGCCCAGTTCTGCATCTCCAGGAAAGACAAACAGTCTCCAGTTTTGGGCCC
AGCTTTCCTAGTCTCTTCTTTTCCTTACCCTCAGCCCTGATCTTGTGTTTGTACGGACAG
TGAGCTCACCCTAGGCCTGGACCCAGGCCCAGTTTCCAAAGCAAGCAGCACACAGCGCAT
GTTCACATAAGCATGGGGGCTGGGGGGACACTGGGGCTTACTGATCTTTTTCTAGGGGCC
TCCAGCCCCTGGCACCACCTAGAGGGGAAAGTGAGTCACCCAAACCATTGCCCCTGGGCT
TACGTCGCTGTAAGCTCACACTGGCCCTGCTGTGCCCTCTTTAGTCACAGACAGCGTGTG
AGCTGACTCTGTCCCTTTAATGCCCAGGCTGAGCCCAGTGCCTCCTTGAGTATCTGCTCC
ATCACTGGCGACGCCACAGGTAGGTGTGAATGGAGTAGCCAGGTGAGATTGTCTCCAGGA
AGCCCACAGCAGGATCCTTGATGGTAAGAGGCACATTCTTAGAGGAGCGGTTTAGCACGA
CCACAACAGCAGAGCCATCGGGATGCATCAGTGCCACTGCGTCCAGGTCGTTCTTCTGAC
TGGCAACCAGCCCCACTCTCTGGGAGCCCTCAGGAATGAACTTGCTGAAGTGGCCAAGGT
GGTAGAACATGGGCTGTTNTGTAAACGTGTCCTTGGTGATGTCTACAATGATGGGACTGT
CGACNAATTACGCCCCCATTGGGTCCTCCTTCGGGTTCAGGGCAGGTTCNCAGTCGNTCA
GCCGACCCATGTACAGGAGTTCGG
Restriction Sites NotI-NotI |
ACCN NM_000157
Insert Size 1611 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000157.2, NP_000148.2
RefSeq Size 2324 bp
RefSeq ORF 1611 bp
Locus ID 2629
UniProt ID P04062
Cytogenetics 1q22
Domains Glyco_hydro_30
Protein Families Druggable Genome
Protein Pathways Lysosome, Metabolic pathways, Other glycan degradation, Sphingolipid metabolism
Summary This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2010
Transcript Variant: This variant (1) encodes isoform 1. Variants 1, 2 and 3 encode the same isoform 1.
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Documents
Resources
"NM_000157" in other vectors (6)
SKU Description Size Price
RC216061 GBA (Myc-DDK-tagged)-Human glucosidase, beta, acid (GBA), transcript variant 1 10 ug
$499.00
RC216061L1 Lenti ORF clone of Human glucosidase, beta, acid (GBA), transcript variant 1, Myc-DDK-tagged 10 ug
$799.00
RC216061L2 Lenti ORF clone of Human glucosidase, beta, acid (GBA), transcript variant 1, mGFP tagged 10 ug
$799.00
RC216061L3 Lenti ORF clone of Human glucosidase, beta, acid (GBA), transcript variant 1, Myc-DDK-tagged 10 ug
$799.00
RC216061L4 Lenti ORF clone of Human glucosidase, beta, acid (GBA), transcript variant 1, mGFP tagged 10 ug
$799.00
RG216061 GBA (tGFP-tagged) - Human glucosidase, beta, acid (GBA), transcript variant 1 10 ug
$489.00 $699.00

file-alt Citations

The GBA p.G85E mutation in Korean patients with non-neuronopathic Gaucher disease: founder and neuroprotective effects
Kim, Choi, Kim et al
Orphanet J Rare Dis (2020) 15 (1), 318 DOI: 10.1186/s13023-020-01597-0
Cited by 15
AI Summary: OriGene products, specifically the GBA Myc-DDK-tagged cDNA clone (RC210889), were used in this study to facilitate genetic analysis of GBA mutations in Korean Gaucher disease patients.
FigureFigureFigure
The GBA p.G85E mutation in Korean patients with non-neuronopathic Gaucher disease: founder and neuroprotective effects
Kim, Choi, Kim et al
Research Square (2020)
Snippet: transfected with the pCMV6 vector. For site-directed mutagenesis, human GBA (NM_000157.4) cDNA (SC120080 OriGene, Rockville, MD, USA) was used as a template for the generation of mutants. GBA mutants, with a wild
Pharmacologic properties of high-dose ambroxol in four patients with Gaucher disease and myoclonic epilepsy
Kim, Yum, Heo et al
J. Med. Genet. (2019) 57 (2), 124-131 DOI: 10.1136/jmedgenet-2019-106132
AI Summary: OriGene’s GBA (NM_000157) human cDNA clone (SC120080) was used in this study to generate mutant GBA constructs for in vitro analysis of glucocerebrosidase activity, contributing to the assessment of ambroxol’s pharmacologic effects in Gaucher disease.
Snippet: …More info You can manage your cookie settings via your browser at any time. To learn more about how we use cookies, please see our cookies policy;The GBA (NM_000157) human cDNA clone was obtained (SC120080 OriGene, Rockville, Maryland, USA) and mutant GBA constructs were generated using the PCR-based DpnI-treatment site-directed mutagenesis method. …
Showing 1-3 of 3 papers.
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