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CD61 (ITGB3) (NM_000212) Human Untagged Clone

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SKU
SC120057
ITGB3 (untagged)-Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3)
  $722.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CD61
Synonyms BDPLT2; BDPLT16; BDPLT24; CD61; GP3A; GPIIIa; GT; GT2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC120057 sequence for NM_000212 edited (data generated by NextGen Sequencing)
ATGCGAGCGCGGCCGCGGCCCCGGCCGCTCTGGGCGACTGTGCTGGCGCTGGGGGCGCTG
GCGGGCGTTGGCGTAGGAGGGCCCAACATCTGTACCACGCGAGGTGTGAGCTCCTGCCAG
CAGTGCCTGGCTGTGAGCCCCATGTGTGCCTGGTGCTCTGATGAGGCCCTGCCTCTGGGC
TCACCTCGCTGTGACCTGAAGGAGAATCTGCTGAAGGATAACTGTGCCCCAGAATCCATC
GAGTTCCCAGTGAGTGAGGCCCGAGTACTAGAGGACAGGCCCCTCAGCGACAAGGGCTCT
GGAGACAGCTCCCAGGTCACTCAAGTCAGTCCCCAGAGGATTGCACTCCGGCTCCGGCCA
GATGATTCGAAGAATTTCTCCATCCAAGTGCGGCAGGTGGAGGATTACCCTGTGGACATC
TACTACTTGATGGACCTGTCTTACTCCATGAAGGATGATCTGTGGAGCATCCAGAACCTG
GGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAACCTGCGGATTGGCTTCGGG
GCATTTGTGGACAAGCCTGTGTCACCATACATGTATATCTCCCCACCAGAGGCCCTCGAA
AACCCCTGCTATGATATGAAGACCACCTGCTTGCCCATGTTTGGCTACAAACACGTGCTG
ACGCTAACTGACCAGGTGACCCGCTTCAATGAGGAAGTGAAGAAGCAGAGTGTGTCACGG
AACCGAGATGCCCCAGAGGGTGGCTTTGATGCCATCATGCAGGCTACAGTCTGTGATGAA
AAGATTGGCTGGAGGAATGATGCATCCCACTTGCTGGTGTTTACCACTGATGCCAAGACT
CATATAGCATTGGACGGAAGGCTGGCAGGCATTGTCCAGCCTAATGACGGGCAGTGTCAT
GTTGGTAGTGACAATCATTACTCTGCCTCCACTACCATGGATTATCCCTCTTTGGGGCTG
ATGACTGAGAAGCTATCCCAGAAAAACATCAATTTGATCTTTGCAGTGACTGAAAATGTA
GTCAATCTCTATCAGAACTATAGTGAGCTCATCCCAGGGACCACAGTTGGGGTTCTGTCC
ATGGATTCCAGCAATGTCCTCCAGCTCATTGTTGATGCTTATGGGAAAATCCGTTCTAAA
GTAGAGCTGGAAGTGCGTGACCTCCCTGAAGAGTTGTCTCTATCCTTCAATGCCACCTGC
CTCAACAATGAGGTCATCCCTGGCCTCAAGTCTTGTATGGGACTCAAGATTGGAGACACG
GTGAGCTTCAGCATTGAGGCCAAGGTGCGAGGCTGTCCCCAGGAGAAGGAGAAGTCCTTT
ACCATAAAGCCCGTGGGCTTCAAGGACAGCCTGATCGTCCAGGTCACCTTTGATTGTGAC
TGTGCCTGCCAGGCCCAAGCTGAACCTAATAGCCATCGCTGCAACAATGGCAATGGGACC
TTTGAGTGTGGGGTATGCCGTTGTGGGCCTGGCTGGCTGGGATCCCAGTGTGAGTGCTCA
GAGGAGGACTATCGCCCTTCCCAGCAGGACGAATGCAGCCCCCGGGAGGGTCAGCCCGTC
TGCAGCCAGCGGGGCGAGTGCCTCTGTGGTCAATGTGTCTGCCACAGCAGTGACTTTGGC
AAGATCACGGGCAAGTACTGCGAGCGTGACGACTTCTCCTGTGTCCGCTACAAGGGGGAG
ATGTGCTCAGGCCATGGCCAGTGCAGCTGTGGGGACTGCCTGTGTGACTCCGACTGGACC
GGCTACTACTGCAACTGTACCACGCGTACTGACACCTGCATGTCCAGCAATGGGCTGCTG
TGCAGCGGCCGCGGCAAGTGTGAATGTGGCAGCTGTGTCTGTATCCAGCCGGGCTCCTAT
GGGGACACCTGTGAGAAGTGCCCCACCTGCCCAGATGCCTGCACCTTTAAGAAAGAATGT
GTGGAGTGTAAGAAGTTTGACCGGGGAGCCCTACATGACGAAAATACCTGCAACCGTTAC
TGCCGTGACGAGATTGAGTCAGTGAAAGAGCTTAAGGACACTGGCAAGGATGCAGTGAAT
TGTACCTATAAGAATGAGGATGACTGTGTCGTCAGATTCCAGTACTATGAAGATTCTAGT
GGAAAGTCCATCCTGTATGTGGTAGAAGAGCCAGAGTGTCCCAAGGGCCCTGACATCCTG
GTGGTCCTGCTCTCAGTGATGGGGGCCATTCTGCTCATTGGCCTTGCCGCCCTGCTCATC
TGGAAACTCCTCATCACCATCCACGACCGAAAAGAATTCGCTAAATTTGAGGAAGAACGC
GCCAGAGCAAAATGGGACACAGCCAACAACCCACTGTATAAAGAGGCCACGTCTACCTTC
ACCAATATCACGTACCGGGGCACTTAA

Clone variation with respect to NM_000212.2
1645 t=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000212 unedited
NNAAGTTAGGATTTGTATACCATCATATAGGCGGCGCGCCAATTCGCACGAGCGGAGCGC
CGCGGGAGGCGGACGAAATGCGAGCGCGGGGGCGGCCCCNGGCGCTCTGGGCGACTGTGC
TGGCGCTGGGGGCGCTGGCGGGCGTTGGCGTANGAGGGGCCAACATCTGTGCCACGCGAG
GTGTGAGCTCCTGCGAGCAGTGCCTGGCTGTGAGCCCCATGTGTGCCTGGTGCTCTGAGG
AGGCCCTGCGCTCTGGGCTCACCTCGCTGTGACCTGAANGAGAATCTGCGGAAGGAGAAC
TGTGCCCCAGAATCCATCGAGTTCCCAGTGAGTGAGGCGCGAGTACTAGAGGACAGGGCC
CTCAGCGACAAGGGCTCTGGAGACAGCTCCCAGGTGCACTCAAGTCAGTCCCCAGAGGAT
TGCACTCCGGCTCCGGGCAGATGATTCGAAGAATTTCTCCATCCAAGTGCGGCAGGTGGA
GGAGTACCCTGTGGACATCTACTACTTGAGGGAGCTGGCTTACTCCATGAGGGAGGATCT
GTGGAGCATCCAGAACCTGGGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAA
CCTGCGGATTGGCTTCNGGGCATTTGTGGACAAGCCTGTGTGACCATACATGTATATCTC
CCCACCAGAGGCGCTCGAAAACCCCTGGTATGATATGAAGACCACCTGCGTGCCCATGTT
TGGCTACAAACACGTGCTGACGCTAACTGAGCANGTGAGCCGCTTCAATGAGGAAGTGAA
AAAGCANAGTGTGTCACGGAACCGAGATGCGCCANAGGGTGGCGTTGATGCCATCATGCA
GGCTACAGTCTGTGATGAAAAAATTGGCTGGAGGAATGAGCATCCCACTTGCTGGTGTGT
ACACTGATGCCA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000212 unedited
AGTNCCCACACACAACACCACACACATAAACACACAGNACCCTCCCATACACACACACAG
NACCCACACTTCCACATACTGACATTCTCCCAACCTACCCCACTCTGAGCCCCAAATCCC
TCCCCACAAATACTGTCCTCTGTAAACATGATGGCAGGGACTCCTGCAATCGTGGCACAG
GCTGATAATGATCTTAGTATGACTGCTTATCATTAAGTGCCCCGGTACGTGATATTGGTG
AAGGTAGACGTGGCCTCTTTATACAGTGGGTTGTTGGCTGTGTCCCATTTTGCTCTGGCG
CGTTCTTCCTCAAATTTAGCGAATTCTTTTCGGTCGTGGATGGTGATGAGGAGTTTCCAG
ATGAGCAGGGCGGCAAGGCCAATGAGCAGAATGGCCCCCATCACTGAGAGCAGGACCACC
AGGATGTCAGGGCCCTTGGGACACTCTGGCTCTTCTACCACATACAGGATGGACTTTCCA
CTAGAATCTTCATAGTACTGGAATCTGACGACACAGTCATCCTCATTCTTATAGGTACAA
TTCACTGCATCCTTGCCAGTGTCCTTAAGCTCTTTCACTGACTCAATCTCGTCACGGCAG
TAACGGTTGCAGGTATTTTCGTCATGTAGGGCTCCCCGGTCAAACTTCTTACACTCCACA
CATTCTTTCTTAAAGGTGCAGGCATCTGGGCAGGTGGNGCACTTCTCACAGGTGTCCCCA
TAGGAGCCCGGCTGGATACAGACACAGCTGCCACATTCACACTTGCCGCGGCCGCTGCAC
AGCAGCCCATTGCTGGACATGCAGGTGTCAGTACGCGTGGTACAGTTGCAGTAGTAGCCC
G
Restriction Sites NotI-NotI |
ACCN NM_000212
Insert Size 2900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000212.2, NP_000203.2
RefSeq Size 4894 bp
RefSeq ORF 2367 bp
Locus ID 3690
UniProt ID P05106
Cytogenetics 17q21.32
Domains EGF, INB
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
Summary The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000212" in other vectors (6)
SKU Description Size Price
RC221606 ITGB3 (Myc-DDK-tagged)-Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3) 10 ug
$722.00
RC221606L1 Lenti ORF clone of Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3), Myc-DDK-tagged 10 ug
$1,022.00
RC221606L2 Lenti ORF clone of Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3), mGFP tagged 10 ug
$1,022.00
RC221606L3 Lenti ORF clone of Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3), Myc-DDK-tagged 10 ug
$1,022.00
RC221606L4 Lenti ORF clone of Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3), mGFP tagged 10 ug
$1,022.00
RG221606 ITGB3 (tGFP-tagged) - Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3) 10 ug
$789.00 $922.00

file-alt Citations

Vitronectin is Involved in the Morphological Transition of Neurites in Retinoic Acid-Induced Neurogenesis of Neuroblastoma Cell Line Neuro2a
Sugahara, Nakaoki, Yamaguchi et al
Neurochem. Res. (2019)
AI Summary: OriGene products contributed to this study by providing the integrin expression plasmid vectors (pCMV6-XL-β3 integrin, pCMV6-XL-β5 integrin, and pCMV6-XL plasmid vector), which were used to investigate the role of integrins in vitronectin-mediated neurite morphological transitions in Neuro2a cells.
Snippet: incubated for 5 h. Transfection of Vectors Integrin expression plasmid vectors (pCMV6-XL-β3 inte- grin, pCMV6-XL-β5 integrin, or pCMV6-XL plasmid vector) were obtained from OriGene (Rockville, MD). A dominant negative Par6
Showing 1-1 of 1 papers.
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