CD61 (ITGB3) (NM_000212) Human Untagged Clone

CAT#: SC120057

ITGB3 (untagged)-Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD61
• Synonyms: BDPLT2; BDPLT16; BDPLT24; CD61; GP3A; GPIIIa; GT; GT2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120057 sequence for NM_000212 edited (data generated by NextGen Sequencing)
  ATGCGAGCGCGGCCGCGGCCCCGGCCGCTCTGGGCGACTGTGCTGGCGCTGGGGGCGCTG
  GCGGGCGTTGGCGTAGGAGGGCCCAACATCTGTACCACGCGAGGTGTGAGCTCCTGCCAG
  CAGTGCCTGGCTGTGAGCCCCATGTGTGCCTGGTGCTCTGATGAGGCCCTGCCTCTGGGC
  TCACCTCGCTGTGACCTGAAGGAGAATCTGCTGAAGGATAACTGTGCCCCAGAATCCATC
  GAGTTCCCAGTGAGTGAGGCCCGAGTACTAGAGGACAGGCCCCTCAGCGACAAGGGCTCT
  GGAGACAGCTCCCAGGTCACTCAAGTCAGTCCCCAGAGGATTGCACTCCGGCTCCGGCCA
  GATGATTCGAAGAATTTCTCCATCCAAGTGCGGCAGGTGGAGGATTACCCTGTGGACATC
  TACTACTTGATGGACCTGTCTTACTCCATGAAGGATGATCTGTGGAGCATCCAGAACCTG
  GGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAACCTGCGGATTGGCTTCGGG
  GCATTTGTGGACAAGCCTGTGTCACCATACATGTATATCTCCCCACCAGAGGCCCTCGAA
  AACCCCTGCTATGATATGAAGACCACCTGCTTGCCCATGTTTGGCTACAAACACGTGCTG
  ACGCTAACTGACCAGGTGACCCGCTTCAATGAGGAAGTGAAGAAGCAGAGTGTGTCACGG
  AACCGAGATGCCCCAGAGGGTGGCTTTGATGCCATCATGCAGGCTACAGTCTGTGATGAA
  AAGATTGGCTGGAGGAATGATGCATCCCACTTGCTGGTGTTTACCACTGATGCCAAGACT
  CATATAGCATTGGACGGAAGGCTGGCAGGCATTGTCCAGCCTAATGACGGGCAGTGTCAT
  GTTGGTAGTGACAATCATTACTCTGCCTCCACTACCATGGATTATCCCTCTTTGGGGCTG
  ATGACTGAGAAGCTATCCCAGAAAAACATCAATTTGATCTTTGCAGTGACTGAAAATGTA
  GTCAATCTCTATCAGAACTATAGTGAGCTCATCCCAGGGACCACAGTTGGGGTTCTGTCC
  ATGGATTCCAGCAATGTCCTCCAGCTCATTGTTGATGCTTATGGGAAAATCCGTTCTAAA
  GTAGAGCTGGAAGTGCGTGACCTCCCTGAAGAGTTGTCTCTATCCTTCAATGCCACCTGC
  CTCAACAATGAGGTCATCCCTGGCCTCAAGTCTTGTATGGGACTCAAGATTGGAGACACG
  GTGAGCTTCAGCATTGAGGCCAAGGTGCGAGGCTGTCCCCAGGAGAAGGAGAAGTCCTTT
  ACCATAAAGCCCGTGGGCTTCAAGGACAGCCTGATCGTCCAGGTCACCTTTGATTGTGAC
  TGTGCCTGCCAGGCCCAAGCTGAACCTAATAGCCATCGCTGCAACAATGGCAATGGGACC
  TTTGAGTGTGGGGTATGCCGTTGTGGGCCTGGCTGGCTGGGATCCCAGTGTGAGTGCTCA
  GAGGAGGACTATCGCCCTTCCCAGCAGGACGAATGCAGCCCCCGGGAGGGTCAGCCCGTC
  TGCAGCCAGCGGGGCGAGTGCCTCTGTGGTCAATGTGTCTGCCACAGCAGTGACTTTGGC
  AAGATCACGGGCAAGTACTGCGAGCGTGACGACTTCTCCTGTGTCCGCTACAAGGGGGAG
  ATGTGCTCAGGCCATGGCCAGTGCAGCTGTGGGGACTGCCTGTGTGACTCCGACTGGACC
  GGCTACTACTGCAACTGTACCACGCGTACTGACACCTGCATGTCCAGCAATGGGCTGCTG
  TGCAGCGGCCGCGGCAAGTGTGAATGTGGCAGCTGTGTCTGTATCCAGCCGGGCTCCTAT
  GGGGACACCTGTGAGAAGTGCCCCACCTGCCCAGATGCCTGCACCTTTAAGAAAGAATGT
  GTGGAGTGTAAGAAGTTTGACCGGGGAGCCCTACATGACGAAAATACCTGCAACCGTTAC
  TGCCGTGACGAGATTGAGTCAGTGAAAGAGCTTAAGGACACTGGCAAGGATGCAGTGAAT
  TGTACCTATAAGAATGAGGATGACTGTGTCGTCAGATTCCAGTACTATGAAGATTCTAGT
  GGAAAGTCCATCCTGTATGTGGTAGAAGAGCCAGAGTGTCCCAAGGGCCCTGACATCCTG
  GTGGTCCTGCTCTCAGTGATGGGGGCCATTCTGCTCATTGGCCTTGCCGCCCTGCTCATC
  TGGAAACTCCTCATCACCATCCACGACCGAAAAGAATTCGCTAAATTTGAGGAAGAACGC
  GCCAGAGCAAAATGGGACACAGCCAACAACCCACTGTATAAAGAGGCCACGTCTACCTTC
  ACCAATATCACGTACCGGGGCACTTAA
  
  Clone variation with respect to NM_000212.2
  1645 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000212 unedited
  NNAAGTTAGGATTTGTATACCATCATATAGGCGGCGCGCCAATTCGCACGAGCGGAGCGC
  CGCGGGAGGCGGACGAAATGCGAGCGCGGGGGCGGCCCCNGGCGCTCTGGGCGACTGTGC
  TGGCGCTGGGGGCGCTGGCGGGCGTTGGCGTANGAGGGGCCAACATCTGTGCCACGCGAG
  GTGTGAGCTCCTGCGAGCAGTGCCTGGCTGTGAGCCCCATGTGTGCCTGGTGCTCTGAGG
  AGGCCCTGCGCTCTGGGCTCACCTCGCTGTGACCTGAANGAGAATCTGCGGAAGGAGAAC
  TGTGCCCCAGAATCCATCGAGTTCCCAGTGAGTGAGGCGCGAGTACTAGAGGACAGGGCC
  CTCAGCGACAAGGGCTCTGGAGACAGCTCCCAGGTGCACTCAAGTCAGTCCCCAGAGGAT
  TGCACTCCGGCTCCGGGCAGATGATTCGAAGAATTTCTCCATCCAAGTGCGGCAGGTGGA
  GGAGTACCCTGTGGACATCTACTACTTGAGGGAGCTGGCTTACTCCATGAGGGAGGATCT
  GTGGAGCATCCAGAACCTGGGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAA
  CCTGCGGATTGGCTTCNGGGCATTTGTGGACAAGCCTGTGTGACCATACATGTATATCTC
  CCCACCAGAGGCGCTCGAAAACCCCTGGTATGATATGAAGACCACCTGCGTGCCCATGTT
  TGGCTACAAACACGTGCTGACGCTAACTGAGCANGTGAGCCGCTTCAATGAGGAAGTGAA
  AAAGCANAGTGTGTCACGGAACCGAGATGCGCCANAGGGTGGCGTTGATGCCATCATGCA
  GGCTACAGTCTGTGATGAAAAAATTGGCTGGAGGAATGAGCATCCCACTTGCTGGTGTGT
  ACACTGATGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000212 unedited
  AGTNCCCACACACAACACCACACACATAAACACACAGNACCCTCCCATACACACACACAG
  NACCCACACTTCCACATACTGACATTCTCCCAACCTACCCCACTCTGAGCCCCAAATCCC
  TCCCCACAAATACTGTCCTCTGTAAACATGATGGCAGGGACTCCTGCAATCGTGGCACAG
  GCTGATAATGATCTTAGTATGACTGCTTATCATTAAGTGCCCCGGTACGTGATATTGGTG
  AAGGTAGACGTGGCCTCTTTATACAGTGGGTTGTTGGCTGTGTCCCATTTTGCTCTGGCG
  CGTTCTTCCTCAAATTTAGCGAATTCTTTTCGGTCGTGGATGGTGATGAGGAGTTTCCAG
  ATGAGCAGGGCGGCAAGGCCAATGAGCAGAATGGCCCCCATCACTGAGAGCAGGACCACC
  AGGATGTCAGGGCCCTTGGGACACTCTGGCTCTTCTACCACATACAGGATGGACTTTCCA
  CTAGAATCTTCATAGTACTGGAATCTGACGACACAGTCATCCTCATTCTTATAGGTACAA
  TTCACTGCATCCTTGCCAGTGTCCTTAAGCTCTTTCACTGACTCAATCTCGTCACGGCAG
  TAACGGTTGCAGGTATTTTCGTCATGTAGGGCTCCCCGGTCAAACTTCTTACACTCCACA
  CATTCTTTCTTAAAGGTGCAGGCATCTGGGCAGGTGGNGCACTTCTCACAGGTGTCCCCA
  TAGGAGCCCGGCTGGATACAGACACAGCTGCCACATTCACACTTGCCGCGGCCGCTGCAC
  AGCAGCCCATTGCTGGACATGCAGGTGTCAGTACGCGTGGTACAGTTGCAGTAGTAGCCC
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000212
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000212.2, NP_000203.2
• RefSeq Size: 4894 bp
• RefSeq ORF: 2367 bp
• Locus ID: 3690
• UniProt ID: P05106
• Cytogenetics: 17q21.32
• Domains: INB, EGF
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• Gene Summary: The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]