CD61 (ITGB3) (NM_000212) Human Untagged Clone

SKU SC120057

ITGB3 (untagged)-Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD61
• Synonyms: BDPLT2; BDPLT16; BDPLT24; CD61; GP3A; GPIIIa; GT; GT2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120057 sequence for NM_000212 edited (data generated by NextGen Sequencing); ATGCGAGCGCGGCCGCGGCCCCGGCCGCTCTGGGCGACTGTGCTGGCGCTGGGGGCGCTG; GCGGGCGTTGGCGTAGGAGGGCCCAACATCTGTACCACGCGAGGTGTGAGCTCCTGCCAG; CAGTGCCTGGCTGTGAGCCCCATGTGTGCCTGGTGCTCTGATGAGGCCCTGCCTCTGGGC; TCACCTCGCTGTGACCTGAAGGAGAATCTGCTGAAGGATAACTGTGCCCCAGAATCCATC; GAGTTCCCAGTGAGTGAGGCCCGAGTACTAGAGGACAGGCCCCTCAGCGACAAGGGCTCT; GGAGACAGCTCCCAGGTCACTCAAGTCAGTCCCCAGAGGATTGCACTCCGGCTCCGGCCA; GATGATTCGAAGAATTTCTCCATCCAAGTGCGGCAGGTGGAGGATTACCCTGTGGACATC; TACTACTTGATGGACCTGTCTTACTCCATGAAGGATGATCTGTGGAGCATCCAGAACCTG; GGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAACCTGCGGATTGGCTTCGGG; GCATTTGTGGACAAGCCTGTGTCACCATACATGTATATCTCCCCACCAGAGGCCCTCGAA; AACCCCTGCTATGATATGAAGACCACCTGCTTGCCCATGTTTGGCTACAAACACGTGCTG; ACGCTAACTGACCAGGTGACCCGCTTCAATGAGGAAGTGAAGAAGCAGAGTGTGTCACGG; AACCGAGATGCCCCAGAGGGTGGCTTTGATGCCATCATGCAGGCTACAGTCTGTGATGAA; AAGATTGGCTGGAGGAATGATGCATCCCACTTGCTGGTGTTTACCACTGATGCCAAGACT; CATATAGCATTGGACGGAAGGCTGGCAGGCATTGTCCAGCCTAATGACGGGCAGTGTCAT; GTTGGTAGTGACAATCATTACTCTGCCTCCACTACCATGGATTATCCCTCTTTGGGGCTG; ATGACTGAGAAGCTATCCCAGAAAAACATCAATTTGATCTTTGCAGTGACTGAAAATGTA; GTCAATCTCTATCAGAACTATAGTGAGCTCATCCCAGGGACCACAGTTGGGGTTCTGTCC; ATGGATTCCAGCAATGTCCTCCAGCTCATTGTTGATGCTTATGGGAAAATCCGTTCTAAA; GTAGAGCTGGAAGTGCGTGACCTCCCTGAAGAGTTGTCTCTATCCTTCAATGCCACCTGC; CTCAACAATGAGGTCATCCCTGGCCTCAAGTCTTGTATGGGACTCAAGATTGGAGACACG; GTGAGCTTCAGCATTGAGGCCAAGGTGCGAGGCTGTCCCCAGGAGAAGGAGAAGTCCTTT; ACCATAAAGCCCGTGGGCTTCAAGGACAGCCTGATCGTCCAGGTCACCTTTGATTGTGAC; TGTGCCTGCCAGGCCCAAGCTGAACCTAATAGCCATCGCTGCAACAATGGCAATGGGACC; TTTGAGTGTGGGGTATGCCGTTGTGGGCCTGGCTGGCTGGGATCCCAGTGTGAGTGCTCA; GAGGAGGACTATCGCCCTTCCCAGCAGGACGAATGCAGCCCCCGGGAGGGTCAGCCCGTC; TGCAGCCAGCGGGGCGAGTGCCTCTGTGGTCAATGTGTCTGCCACAGCAGTGACTTTGGC; AAGATCACGGGCAAGTACTGCGAGCGTGACGACTTCTCCTGTGTCCGCTACAAGGGGGAG; ATGTGCTCAGGCCATGGCCAGTGCAGCTGTGGGGACTGCCTGTGTGACTCCGACTGGACC; GGCTACTACTGCAACTGTACCACGCGTACTGACACCTGCATGTCCAGCAATGGGCTGCTG; TGCAGCGGCCGCGGCAAGTGTGAATGTGGCAGCTGTGTCTGTATCCAGCCGGGCTCCTAT; GGGGACACCTGTGAGAAGTGCCCCACCTGCCCAGATGCCTGCACCTTTAAGAAAGAATGT; GTGGAGTGTAAGAAGTTTGACCGGGGAGCCCTACATGACGAAAATACCTGCAACCGTTAC; TGCCGTGACGAGATTGAGTCAGTGAAAGAGCTTAAGGACACTGGCAAGGATGCAGTGAAT; TGTACCTATAAGAATGAGGATGACTGTGTCGTCAGATTCCAGTACTATGAAGATTCTAGT; GGAAAGTCCATCCTGTATGTGGTAGAAGAGCCAGAGTGTCCCAAGGGCCCTGACATCCTG; GTGGTCCTGCTCTCAGTGATGGGGGCCATTCTGCTCATTGGCCTTGCCGCCCTGCTCATC; TGGAAACTCCTCATCACCATCCACGACCGAAAAGAATTCGCTAAATTTGAGGAAGAACGC; GCCAGAGCAAAATGGGACACAGCCAACAACCCACTGTATAAAGAGGCCACGTCTACCTTC; ACCAATATCACGTACCGGGGCACTTAA; ; Clone variation with respect to NM_000212.2; 1645 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000212 unedited; NNAAGTTAGGATTTGTATACCATCATATAGGCGGCGCGCCAATTCGCACGAGCGGAGCGC; CGCGGGAGGCGGACGAAATGCGAGCGCGGGGGCGGCCCCNGGCGCTCTGGGCGACTGTGC; TGGCGCTGGGGGCGCTGGCGGGCGTTGGCGTANGAGGGGCCAACATCTGTGCCACGCGAG; GTGTGAGCTCCTGCGAGCAGTGCCTGGCTGTGAGCCCCATGTGTGCCTGGTGCTCTGAGG; AGGCCCTGCGCTCTGGGCTCACCTCGCTGTGACCTGAANGAGAATCTGCGGAAGGAGAAC; TGTGCCCCAGAATCCATCGAGTTCCCAGTGAGTGAGGCGCGAGTACTAGAGGACAGGGCC; CTCAGCGACAAGGGCTCTGGAGACAGCTCCCAGGTGCACTCAAGTCAGTCCCCAGAGGAT; TGCACTCCGGCTCCGGGCAGATGATTCGAAGAATTTCTCCATCCAAGTGCGGCAGGTGGA; GGAGTACCCTGTGGACATCTACTACTTGAGGGAGCTGGCTTACTCCATGAGGGAGGATCT; GTGGAGCATCCAGAACCTGGGTACCAAGCTGGCCACCCAGATGCGAAAGCTCACCAGTAA; CCTGCGGATTGGCTTCNGGGCATTTGTGGACAAGCCTGTGTGACCATACATGTATATCTC; CCCACCAGAGGCGCTCGAAAACCCCTGGTATGATATGAAGACCACCTGCGTGCCCATGTT; TGGCTACAAACACGTGCTGACGCTAACTGAGCANGTGAGCCGCTTCAATGAGGAAGTGAA; AAAGCANAGTGTGTCACGGAACCGAGATGCGCCANAGGGTGGCGTTGATGCCATCATGCA; GGCTACAGTCTGTGATGAAAAAATTGGCTGGAGGAATGAGCATCCCACTTGCTGGTGTGT; ACACTGATGCCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000212 unedited; AGTNCCCACACACAACACCACACACATAAACACACAGNACCCTCCCATACACACACACAG; NACCCACACTTCCACATACTGACATTCTCCCAACCTACCCCACTCTGAGCCCCAAATCCC; TCCCCACAAATACTGTCCTCTGTAAACATGATGGCAGGGACTCCTGCAATCGTGGCACAG; GCTGATAATGATCTTAGTATGACTGCTTATCATTAAGTGCCCCGGTACGTGATATTGGTG; AAGGTAGACGTGGCCTCTTTATACAGTGGGTTGTTGGCTGTGTCCCATTTTGCTCTGGCG; CGTTCTTCCTCAAATTTAGCGAATTCTTTTCGGTCGTGGATGGTGATGAGGAGTTTCCAG; ATGAGCAGGGCGGCAAGGCCAATGAGCAGAATGGCCCCCATCACTGAGAGCAGGACCACC; AGGATGTCAGGGCCCTTGGGACACTCTGGCTCTTCTACCACATACAGGATGGACTTTCCA; CTAGAATCTTCATAGTACTGGAATCTGACGACACAGTCATCCTCATTCTTATAGGTACAA; TTCACTGCATCCTTGCCAGTGTCCTTAAGCTCTTTCACTGACTCAATCTCGTCACGGCAG; TAACGGTTGCAGGTATTTTCGTCATGTAGGGCTCCCCGGTCAAACTTCTTACACTCCACA; CATTCTTTCTTAAAGGTGCAGGCATCTGGGCAGGTGGNGCACTTCTCACAGGTGTCCCCA; TAGGAGCCCGGCTGGATACAGACACAGCTGCCACATTCACACTTGCCGCGGCCGCTGCAC; AGCAGCCCATTGCTGGACATGCAGGTGTCAGTACGCGTGGTACAGTTGCAGTAGTAGCCC; G;
• Restriction Sites: NotI-NotI
• ACCN: NM_000212
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000212.2, NP_000203.2
• RefSeq Size: 4894 bp
• RefSeq ORF: 2367 bp
• Locus ID: 3690
• UniProt ID: P05106
• Cytogenetics: 17q21.32
• Domains: EGF, INB
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• Summary: The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]