beta Sarcoglycan (SGCB) (NM_000232) Human Untagged Clone

SKU SC120022

SGCB (untagged)-Human sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) (SGCB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: beta Sarcoglycan
• Synonyms: A3b; LGMD2E; LGMDR4; SGC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000232, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCAGCGGCGGCGGCGGCTGCAGAACAGCAAAGTTCCAATGGTCCTGTAAAGAAGTCCATGCGTG; AGAAGGCTGTTGAGAGAAGGAGTGTCAATAAAGAGCACAACAGTAACTTTAAAGCTGGATACATTCCGAT; TGATGAAGATCGTCTCCACAAAACAGGGTTGAGAGGAAGAAAGGGCAATTTAGCCATCTGTGTGATTATC; CTCTTGTTTATCCTGGCTGTCATCAATTTAATAATAACACTTGTTATTTGGGCCGTGATTCGCATTGGAC; CAAATGGCTGTGATAGTATGGAGTTTCATGAAAGTGGCCTGCTTCGATTTAAGCAAGTATCTGACATGGG; AGTGATCCACCCTCTTTATAAAAGCACAGTAGGAGGAAGGCGAAATGAAAATTTGGTCATCACTGGCAAC; AACCAGCCTATTGTTTTTCAGCAAGGGACAACAAAGCTCAGTGTAGAAAACAACAAAACTTCTATTACAA; GTGACATCGGCATGCAGTTTTTTGACCCGAGGACTCAAAATATCTTATTCAGCACAGACTATGAAACTCA; TGAGTTTCATTTGCCAAGTGGAGTGAAAAGTTTGAATGTTCAAAAGGCATCTACTGAAAGGATTACCAGC; AATGCTACCAGTGATTTAAATATAAAAGTTGATGGGCGTGCTATTGTGCGTGGAAATGAAGGTGTATTCA; TTATGGGCAAAACCATTGAATTTCACATGGGTGGTAATATGGAGTTAAAGGCGGAAAACAGTATCATCCT; AAATGGATCTGTGATGGTCAGCACCACCCGCCTACCCAGTTCCTCCAGTGGAGACCAGTTGGGTAGTGGT; GACTGGGTACGCTACAAGCTCTGCATGTGTGCTGATGGGACGCTCTTCAAGGTGCAAGTAACCAGCCAGA; ACATGGGCTGCCAAATCTCAGACAACCCCTGTGGAAACACTCATTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000232 unedited; TTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGAAGATGGCGGCA; GCGGCGGCGGCGGCTGCAGAACAGCAAAGTTCCAATGGTCCTGTAAAGAAGTCCATGCGT; GAGAAGGCTGTTGAGAGAAGGAGTGTCAATAAAGAGCACAACAGTAACTTTAAAGCTGGA; TACATTCCGATTGATGAAGATCGTCTCCACAAAACAGGGTTGAGAGGAAGAAAGGGCAAT; TTAGCCATCTGTGTGATTATCCTCTTGTTTATCCTGGCTGTCATCAATTTAATAATAACA; CTTGTTATTTGGGCCGTGATTCGCATTGGACCAAATGGCTGTGATAGTATGGAGTTTCAT; GAAAGTGGCCTGCTTCGATTTAAGCAAGTATCTGACATGGGAGTGATCCACCCTCTTTAT; AAAAGCACAGTAGGAGGAAGGCGAAATGAAAATTTGGTCATCACTGGCAACAACCAGCCT; ATTGTTTTTCAGCAAGGGACAACAAAGCTCAGTGTAGAAAACAACAAAACTTCTATTACA; AGTGACATCGGCATGCAGTTTTTTGACCCGAGGACTCAAAATATCTTATTCAGCACAGAC; TATGAAACTCATGAGTTTCATTTGCCAAGTGGAGTGAAAAGTTTGAATGTTCAAAAGGCA; TCTACTGAAAGGGATTACCAGCATGCTACCAGTGATTTAATATAAAAGTGATNGGGCGTG; CTATNGGTGCGTGGAAATGAANGGTGTATTCATTATGGGCAAAACCATTGAATTTACATG; GNGTGGTATNNATGGAGTTAAAGGCGGNAAACAGTATCATCTAAATGGATCTGTGATGGT; CAGCACCACCCGCTACCCANTNNNCTCATGAGACANNTGGGGNANTGGGGACTTGGGACC; TACAGCTCTGGATGGTGCTGATGGGACCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000232 unedited; CCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAATTTACAGAATTTTT; ATTGTAAACAGAAGCTCATTACTAGTTATTACAAACTGAATCAACTGAAACTCAAGTTAG; CAATGTGAAACACATTTATAGGCTTATTTTTGTATACCATAATTGGTAATTTTTGCCCCC; TCAATACTTTTAAAAAGACTGTTAAAATTTTCAGCATTTCAATGATCTCTTATTTGTCAG; GTTTAGCACCATGACATTCTCATTCCCTTAAAAAAACACTCATTCAAATCTAGGTAGAAC; TTCCACCTTTCATCATCAAAATTATCACACAAAATTTATTTTGTTTTTTTCACAATGCAA; AAAGAAGACAAATAAGTATATTTTAAAATATTATCAGCAGTCCGTTTTGTGTGGTTTTAC; ATTTTTCTGTCTGCCCATTATTAAAAAATCTTGCATTGATTTCATTTGCCCCCCTTGTCA; TTGCAACCCGTCTCTAGAATACTGTGGTAATTGAAACTAGCATGTACCTGCGCCCCCTCC; CGGGGTCCTATCTTTGTCACCCACCTCCCCCATGGCTACTGCTTGTCATATCTGTGCTCA; GTGCAGGGATGAGCATACAGCAGGAAGAGGCGAAGTCAGCATTTCATTTACAGAAAAATA; CTATCTGGTGGAGACAATGAGTTGCTGAAGATTTACTGTGGATAAAAATAAGCCGGCCAG; GCGTGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCACGA; TGTCAAGAGATTGAGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATAC; AAAAATTANCCCGGGACTGTGGCACATGCCTGTAGTCCCAGTACTCGGGAAGGCTGAGCA; GAGAATCGCTTGACCCCTGGAGCANAGNTGCATGATCCANAGCGATTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000232
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000232.3, NP_000223.1
• RefSeq Size: 4229 bp
• RefSeq ORF: 957 bp
• Locus ID: 6443
• UniProt ID: Q16585
• Cytogenetics: 4q12
• Domains: sarcoglycan
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis
• Summary: This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with limb-girdle muscular dystrophy.[provided by RefSeq, Oct 2008]