hCG (CGA) (NM_000735) Human Untagged Clone

SKU SC119734

CGA (untagged)-Human glycoprotein hormones, alpha polypeptide (CGA)

Product Data

• Type: Human Untagged Clone
• Target Symbol: hCG
• Synonyms: CG-ALPHA; FSHA; GPA1; GPHa; GPHA1; HCG; LHA; TSHA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119734 sequence for NM_000735 edited (data generated by NextGen Sequencing); ATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATTGTCGGTGTTTCTGCAT; GTTCTCCATTCCGCTCCTGATGTGCAGGATTGCCCAGAATGCACGCTACAGGAAAACCCA; TTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCTGCTGCTTCTCTAGAGCA; TATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAAAGAACGTCACCTCAGAG; TCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTG; GAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAA; ; Clone variation with respect to NM_000735.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000735 unedited; GCTGGATTTAGGNAATCCGNATTTCACTATAGGGCGAGCCGCGCAATTCGGCACCAGNAC; GAAGCTAAAATCCCTCTTCGGATCCACAGTCAACCGCCCTGAACACTCCTGCAAAAAGCC; CAGAGAAAGGAGCGCCATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATT; GTCGGTGTTTCTGCATGTTCTCCATTCCGCTCCTGTATGTTCAGGATTGCCCAGAATGCA; CGCTACAGGAAAACCCATTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCT; GCTGCTTCTCTAGAGCATATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAA; AGAACGTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAA; TGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA; AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT; TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC; TTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA; ATCAGCAGTCTAGTTCTTTTCATTTGGGATGAATACAGCATTTAGCTTGGTCCACTGCAA; ATAAAGCCTTTTTAATCATCAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCNGCCGCG; GTCATAGCTGTTTCCCTGACAGATCCCGGGTGGCATCCCTGTGACCCTCCCCAATGCCTT; TCTGGGCCTGGAAGTGCCCCTCCAGTGCCAACAAACTTGGGCCTATAAATAAAGTGCATC; ATTTTGNCTGACAAGGTGTCCCTCTTAAATTATGGGTTGAGAGGGTTGTGTTGAAAACCA; GGGCAAATTTGGAAAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000735
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000735.2, NP_000726.1
• RefSeq Size: 704 bp
• RefSeq ORF: 351 bp
• Locus ID: 1081
• UniProt ID: P01215
• Cytogenetics: 6q14.3
• Domains: GHA
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Autoimmune thyroid disease, GnRH signaling pathway, Neuroactive ligand-receptor interaction
• Summary: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]; Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.