C4BPA (NM_000715) Human Untagged Clone

SKU SC119723

C4BPA (untagged)-Human complement component 4 binding protein, alpha (C4BPA)

Product Data

• Type: Human Untagged Clone
• Target Symbol: C4BPA
• Synonyms: C4BP; PRP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119723 sequence for NM_000715 edited (data generated by NextGen Sequencing); ATGCACCCCCCAAAAACTCCATCTGGGGCTCTTCATAGAAAAAGGAAAATGGCAGCCTGG; CCCTTCTCCAGGCTGTGGAAAGTCTCTGATCCAATTCTCTTCCAAATGACCTTGATCGCT; GCTCTGTTGCCTGCTGTTCTTGGCAATTGTGGTCCTCCACCCACTTTATCATTTGCTGCC; CCGATGGATATTACGTTGACTGAGACACGCTTCAAAACTGGAACTACTCTGAAATACACC; TGCCTCCCTGGCTACGTCAGATCCCATTCAACTCAGACGCTTACCTGTAATTCTGATGGC; GAATGGGTGTATAACACCTTCTGTATCTACAAACGATGCAGACACCCAGGAGAGTTACGT; AATGGGCAAGTAGAGATTAAGACAGATTTATCTTTTGGATCACAAATAGAATTCAGCTGT; TCAGAAGGATTTTTCTTAATTGGCTCAACCACTAGTCGTTGTGAAGTCCAAGATAGAGGA; GTTGGCTGGAGTCATCCTCTCCCACAATGTGAAATTGTCAAGTGTAAGCCTCCTCCAGAC; ATCAGGAATGGAAGGCACAGCGGTGAAGAAAATTTCTACGCATACGGCTTTTCTGTCACC; TACAGCTGTGACCCCCGCTTCTCACTCTTGGGCCATGCCTCCATTTCTTGCACTGTGGAG; AATGAAACAATAGGCGTTTGGAGACCAAGCCCTCCTACCTGTGAAAAAATCACCTGTCGC; AAGCCAGATGTTTCACATGGGGAAATGGTCTCTGGATTTGGACCCATCTATAATTACAAA; GACACTATTGTGTTTAAGTGCCAAAAAGGTTTTGTTCTCAGAGGCAGCAGTGTAATTCAT; TGTGATGCTGATAGCAAATGGAATCCTTCTCCTCCTGCTTGTGAGCCCAATAGTTGTACT; AATTTACCAGACATTCCACATGCTTCCTGGGAAACATATCCTAGGCCGACAAAAGAGGAT; GTGTATGTTGTTGGGACTGTGTTAAGGTACCGCTGTCATCCTGGCTACAAACCCACTACA; GATGAGCCTACGACTGTGATTTGTCAGAAAAATTTGAGATGGACCCCATACCAAGGATGT; GAGGCGTTATGTTGCCCTGAACCAAAGCTAAATAATGGTGAAATCACTCAACACAGGAAA; AGTCGTCCTGCCAATCACTGTGTTTATTTCTATGGAGATGAGATTTCATTTTCATGTCAT; GAGACCAGTAGGTTTTCAGCTATATGCCAAGGAGATGGCACGTGGAGTCCCCGAACACCA; TCATGTGGAGACATTTGCAATTTTCCTCCTAAAATTGCCCATGGGCATTATAAACAATCT; AGTTCATACAGCTTTTTCAAAGAAGAGATTATATATGAATGTGATAAAGGCTACATTCTG; GTCGGACAGGCGAAACTCTCCTGCAGTTATTCACACTGGTCAGCTCCAGCCCCTCAATGT; AAAGCTCTGTGTCGGAAACCAGAATTAGTGAATGGAAGGTTGTCTGTGGATAAGGATCAG; TATGTTGAGCCTGAAAATGTCACCATCCAATGTGATTCTGGCTATGGTGTGGTTGGTCCC; CAAAGTATCACTTGCTCTGGGAACAGAACCTGGTACCCAGAGGTGCCCAAGTGTGAGTGG; GAGACCCCCGAAGGCTGTGAACAAGTGCTCACAGGCAAAAGACTCATGCAGTGTCTCCCA; AACCCAGAGGATGTGAAAATGGCCCTGGAGGTATATAAGCTGTCTCTGGAAATTGAACAA; CTGGAACTACAGAGAGACAGCGCAAGACAATCCACTTTGGATAAAGAACTATAA; ; Clone variation with respect to NM_000715.3; 675 t=>c;899 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000715 unedited; GGTTGCAGGAATTTGTAATACGATTCACTATAGGGCGGCCGCNGAATCGGCACGAGGTTT; TCTTCTTTGAGAAACTATCCCAGATATCATCATAGAGTCTTCTGCTCTTCCTCAACTACC; AAAGAAAAACATCAGCGAAGCAGCAGGCCATGCACCCCCCAAAAACTCCATCTGGGGCTC; TTCATAGAAAAAGGAAAATGGCAGCCTGGCCCTTCTCCAGGCTGTGGAAAGTCTCTGATC; CAATTCTCTTCCAAATGACCTTGATCGCTGCTCTGTTGCCTGCTGTTCTTGGCAATTGTG; GTCCTCCACCCACTTTATCATTTGCTGCCCCGATGGATATTACGTTGACTGAGACACGCT; TCAAAACTGGAACTACTCTGAAATACACCTGCCTCCCTGGCTACGTCAGATCCCATTCAA; CTCAGACGCTTACCTGTAATTCTGATGGCGAATGGGTGTATAACACCTTCTGTATCTACA; AACGATGCAGACACCCAGGAGAGTTACGTAATGGGCAAGTAGAGATTAAGACAGATTTAT; CTTTTGGATCACAAATAGAATTCAGCTGTTCAGAAGGATTTTTCTTAATTGGCTCAACCA; CTAGTCGTTGTGAAGTCCAAGATAGAGGAGTTGGCTGGAGTCATCCTCTCCCACAATGTG; AAATTGTCAAGTGTAAGCCTCCTCCAGACATCAGGAATGGAAGGCACAGCGGTGAAGAAA; ATTTCTACGCATACGGCTTTTCTGTCACCTACAGCTGTGACCNCCGCTTCTCACTCTTGG; GCCATGCCTCCATTTCTTGCACTGGGGAGAATGAACAATAAGCCGTTGGGAGACCAGCCC; TNCTACCTGTGAAAAATCACCTGTCGCAAGCCAGATGTTTACATGGGGAAAAGGTCTCTG; GATTTGGACCCATCTATAAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000715 unedited; NNTTTTACTCTGNACCCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTAT; ACTACATTTTTAATCGAAAAAAATTTGTGCTTTGTGTGTTGAAAACAAAAACATGAGCCA; CACAGAGGATGATTAATAATTTCACAATCTCAAAGCACTAAACTTTAGCAAATTATCATT; TCTAGATATTTATTATGATGAATATCACTGCCAAATTGACAGTAGATTTGCTAAACTGAT; CTGTATTGAATTGCAAGAGGCAAGCCAGCAAGACACCTTTTCCTCCTTCTTTTGAGAAAA; ATTATAGTTCTTTATCCAAAGTGGATTGTCTTGCGCTGTCTCTCTGTAGTTCCAGTTGTT; CAATTTCCAGAGACAGCTTATATACCTCCAGGGCCATTTTCACATCCTCTGGGTTTGGGA; GACACTGCATGAGTCTTTTGCCTGTGAGCACTTGTTCACAGCCTTCGGGGGTCTCCCACT; CACACTTGGGCACCTCTGGGTACCAGGTTCTGTTCCCAGAGCAAGTGATACTTTGGGGAC; CAACCACACCATAGCCAGAATCACATTGGATGGTGACATTTTCAGGCTCAACATACTGAT; CCTTATCCACAGACAACCTTCCATTCACTAATTCTGGTTTCCGACACAGAGCTTTACATT; GAGGGGCTGGAGCTGACCAGTGTGAATAACTGCAGGAGAGTTTCGCCTGTCCGACCAGAA; TGTAGCCTTTATCACATTCATATATAANTCTCTTCTTTGAANAAGCTGTATGAACTAGAT; TGTTTATAATGCCCATGGGGCATTTAGGAGGGAAAATGCAATGTCTCCCATGATGGTGTC; GGGACTCCCGTGCCATCTCTGGCATTAGCTGAAACTACTGGTCTATGACATGAATGAATC; TCATCTCTAGAATAACCAGTGATGGCAGACACTTTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000715
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000715.3, NP_000706.1
• RefSeq Size: 2256 bp
• RefSeq ORF: 1794 bp
• Locus ID: 722
• UniProt ID: P04003
• Cytogenetics: 1q32.2
• Domains: CCP
• Protein Pathways: Complement and coagulation cascades
• Summary: This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]