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Bak (BAK1) (NM_001188) Human Untagged Clone

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SKU
SC119385
BAK1 (untagged)-Human BCL2-antagonist/killer 1 (BAK1)
  $450.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Bak
Synonyms BAK; BAK-LIKE; BCL2L7; CDN1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>SC119385 representing NM_001188.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCGGCCGCGAATTCGGCACGAGGCACAGGGACAAGTAAAGGCTACATCCAGATGCCGGGAATGCACTGA
CGCCCATTCCTGGAAACTGGGCTCCCACTCAGCCCCTGGGAGCAGCAGCCGCCAGCCCCTCGGGACCTC
CATCTCCACCCTGCTGAGCCACCCGGGTTGGGCCAGGATCCCGGCAGGCTGATCCCGTCCTCCACTGAG
ACCTGAAAAATGGCTTCGGGGCAAGGCCCAGGTCCTCCCAGGCAGGAGTGCGGAGAGCCTGCCCTGCCC
TCTGCTTCTGAGGAGCAGGTAGCCCAGGACACAGAGGAGGTTTTCCGCAGCTACGTTTTTTACCGCCAT
CAGCAGGAACAGGAGGCTGAAGGGGTGGCTGCCCCTGCCGACCCAGAGATGGTCACCTTACCTCTGCAA
CCTAGCAGCACCATGGGGCAGGTGGGACGGCAGCTCGCCATCATCGGGGACGACATCAACCGACGCTAT
GACTCAGAGTTCCAGACCATGTTGCAGCACCTGCAGCCCACGGCAGAGAATGCCTATGAGTACTTCACC
AAGATTGCCACCAGCCTGTTTGAGAGTGGCATCAATTGGGGCCGTGTGGTGGCTCTTCTGGGCTTCGGC
TACCGTCTGGCCCTACACGTCTACCAGCATGGCCTGACTGGCTTCCTAGGCCAGGTGACCCGCTTCGTG
GTCGACTTCATGCTGCATCACTGCATTGCCCGGTGGATTGCACAGAGGGGTGGCTGGGTGGCAGCCCTG
AACTTGGGCAATGGTCCCATCCTGAACGTGCTGGTGGTTCTGGGTGTGGTTCTGTTGGGCCAGTTTGTG
GTACGAAGATTCTTCAAATCATGACTCCCAAGGGTGCCCTTTGGGGTCCCGGTTCAGACCCCTGCCTGG
ACTTAAGCGAAGTCTTTGCCTTCTCTGCTCCCTTGCAGGGGTCCCCCCTCAAGAGTACAGAAGCTTTAG
CAAGTGTGCACTCCAGCTTCGGAGGGCCCCTGCGTGGGGGCCAGTCAGGCTGCAGAGGCACCTCAACAT
TGCATGGTGCTAGTGGGCCCTCTCTCTGGGCCCAGGGGCTGTGGCCGTCTCCTCCCTCAGCTCTCTGGG
ACCTCCTTAGCCCTGTCTGCTAGGCGCTGGGGAGACTGATAACTTGGGGAGGCAAGAGACTGGGAGCCA
CTTCTCCCCAGAAAGTGTTTAACGGTTTTAGCTTTTTATAATACCCTTGTGAGAGCCCATTCCCACCAT
TCTACCTGAGGCCAGGACGTCTGGGGTGTGGGGATTGGTGGGTCTATGTTCCCCAGGATTCAGCTATTC
TGGAAGATCAGCACCCTAAGAGATGGGACTAGGACCTGAGCCTGGTCCTGGCCGTCCCTAAGCATGTGT
CCCAGGAGCAGGACCTACTAGGAGAGGGGGGCCAAGGTCCTGCTCAACTCTACCCCTGCTCCCATTCCT
CCCTCCGGCCATACTGCCTTTGCAGTTGGACTCTCAGGGATTCTGGGCTTGGGGTGTGGGGTGGGGTGG
AGTCGCAGACCAGAGCTGTCTGAACTCACGTGTCAGAAGCCTCCAAGCCTGCCTCCCAAGGTCCTCTCA
GTTCTCTCCCTTCCTCTCTCCTTATAGACACTTGCTCCCAACCCATTCACTACAGGTGAAGGCTCTCAC
CCCCATCCCTGGGGGCCTTGGGTGAGTGGCCTGCTAAGGCTCCTCCTTGCCCAGACTACAGGGCTTAGG
ACTTGGTTTGTTATTTCAGGGAAAAGGAGTAGGGAGTTCATCTGGAGGGTTCTAAGTGGGAGAAGGACT
ATCAACACCACTAGGAATCCCAGAGGTGGGATCCTCCCTCATGGCTCTGGCACAGTGTAATCCAGGGGT
GTAGATGGGGGAACTGTGAATACTTGAACTCTGTTCCCCCACCCTCCATGCTCCTCACCTGTCTAGGTC
TCCTCAGGGTGGGGGGTGACAGTGCCTTCTCTATTGGGCACAGCCTAGGGTCTTGGGGGTCAGGGGGGA
GAAGTTCTTGATTCAGCCAAATGCAGGGAGGGGAGGCAGATGGAGCCCATAGGCCACCCCCTATCCTCT
GAGTGTTTGGAAATAAACTGTGCAATCCCCTCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGC
CGC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001188 unedited
GGTTATAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCACAGG
GACAAGTAAAGGCTACATCCAGATGCCGGGAATGCACTGACGCCCATTCCTGGAAACTGG
GCTCCCACTCAGCCCCTGGGAGCAGCAGCCGCCAGCCCCTCGGGACCTCCATCTCCACCC
TGCTGAGCCACCCGGGTTGGGCCAGGATCCCGGCAGGCTGATCCCGTCCTCCACTGAGAC
CTGAAAAATGGCTTCGGGGCAAGGCCCAGGTCCTCCCAGGCAGGAGTGCGGAGAGCCTGC
CCTGCCCTCTGCTTCTGAGGAGCAGGTAGCCCAGGACACAGAGGAGGTTTTCCGCAGCTA
CGTTTTTTACCGCCATCAGCAGGAACAGGAGGCTGAAGGGGTGGCTGCCCCTGCCGACCC
AGAGATGGTCACCTTACCTCTGCAACCTAGCAGCACCATGGGGCAGGTGGGACGGCAGCT
CGCCATCATCGGGGACGACATCAACCGACGCTATGACTCAGAGTTCCAGACCATGTTGCA
GCACCTGCAGCCCACGGCAGAGAATGCCTATGAGTACTTCACCAAGATTGCCACCAGCCT
GTTTGAGAGTGGCATCAATTGGGGCCGTGTGGTGGCTCTTCTGGGCTTCGGCTACCGTCT
GGCCCTACACGTCTACCAGCATGCCCTGACTGGCTTCCTANGCCANGTGACCCGCTTCGT
GGTCGACTTCATGCTGCATCACTGCATTGCCCGGTGGATTGCACACAGGGCTGCCTGGGT
GGCAGCCCTGAACTTTGGCCATGGTCCCATCCTGAACGTGCTGGTGGNTCCTGGGGTGGG
TCTTGTGGGCCCAGTTGTGTTACGAAAAATCTCAAACATGACTCCCAAGGTGCCCTTTGG
GGTCCCGGTCAGACCCCA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001188 unedited
ATGGCCGCGGCCCGCATCTANGATCGAGTTTTTTTTTTTTTTTTTTGAGGGGATTGCACA
GTTTATTTCCAAACACTCAGNAGATAGGGGGTGGCCTATGGGCTCCATCTGCCTCCCCTC
CCTGCATTTGGCTGAATCAAGAACTTCTCCCCCCTGACCCCCAAGACCCTAGGCTGTGCC
CAATAGAGAAGGCACTGTCACCCCCCACCCTGAGGAGACCTAGACAGGTGAGGAGCATGG
AGGGTGGGGGAACAGAGTTCAAGTATTCACAGTTCCCCCATCTACACCCCTGGATTACAC
TGTGCCAGAGCCATGAGGGAGGATCCCACCTCTGGGATTCCTAGTGGCGTTGATAGTCCT
TCTCCCACTTAGAACCCTCCAGATGAACTCCCTACTCCTTTTCCCTGAAATAACAAACCA
AGTCCTAAGCCCTGTAGTCTGGGCAAGGAGGAGCCTTAGCAGGCCACTCACCCAAGGCCC
CCAGGGATGGGGGTGAGAGCCCTTCACCTGTAGTGAATGGGTTGGGAGCAAGTGTCTATA
AGGAGAGAGGAAGGGAGAGAACTGACAGGACCTTGGGAGGCAGGCTTGGAGGCTTCTGAC
ACGTGAGTTCAGACAGCTCTGGTCTGCGACTTCACCCCACCCACACCCCAAGCCCAGAAT
CCCTGAGAGTTCAACTGCAAAGGCCATATGGCCGGAGGGAGGAATGGCAGCCAGGGTAAA
GTTGACCAGGACCTTGATCACCCTTTACTAAAGGACCTGCTCCTGGGCACATGCTTAGGG
ACGCCCAGCACCAGCCTCAGCTCCTAGACCCATCTCTTAGGGGCTGATCTTACAAAAACC
TGAAATCTGGGGAACATAAACCCCCCATTTCCGACCCCCCGAAGACCTGGACCCAG
Restriction Sites NotI-NotI |
ACCN NM_001188
Insert Size 2142 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001188.2
RefSeq Size 2165 bp
RefSeq ORF 2142 bp
Locus ID 578
UniProt ID Q16611
Cytogenetics 6p21.31
Domains Bcl-2
Protein Families Druggable Genome, Stem cell - Pluripotency, Transmembrane
MW 77.1 kDa
Summary The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001188" in other vectors (6)
SKU Description Size Price
RC201216 BAK1 (Myc-DDK-tagged)-Human BCL2-antagonist/killer 1 (BAK1) 10 ug
$450.00
RC201216L1 Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), Myc-DDK-tagged 10 ug
$750.00
RC201216L2 Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), mGFP tagged 10 ug
$750.00
RC201216L3 Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), Myc-DDK-tagged 10 ug
$750.00
RC201216L4 Lenti ORF clone of Human BCL2-antagonist/killer 1 (BAK1), mGFP tagged 10 ug
$750.00
RG201216 BAK1 (tGFP-tagged) - Human BCL2-antagonist/killer 1 (BAK1) 10 ug
$650.00

file-alt Citations

Notch4 signaling confers susceptibility to TRAIL induced apoptosis in breast cancer cells
Naik, MacFarlane, Sarin
J. Cell. Biochem. (2015)
Cited by 14
AI Summary: OriGene's Bak plasmid was used to generate Bak-RFP constructs, enabling the study of Bak's role in Notch4-mediated sensitization of breast cancer cells to TRAIL-induced apoptosis.
Snippet: Bethesda, MD, USA), respectively. Bcl-xL-RFP [Parikh et al., 2007] and Bak-RFP (Bak plasmid obtained from Origene (Rockville, MD, USA)) were generated in-house. Bcl-2 was purchased from Millipore-Upstate (Charlottesville, VA, USA). Empty vector ... Cite SaveSaving
The C-terminal domain (CTD) in linker histones antagonizes anti-apoptotic proteins to modulate apoptotic outcomes at the mitochondrion
Garg, Ramdas, Vijayalakshmi et al
Cell Death Dis (2014) 5, e1058 DOI: 10.1038/cddis.2014.20
Cited by 9
AI Summary: OriGene contributed Bak ORF constructs, which were PCR amplified and used to generate recombinant Bak proteins for experiments investigating the modulation of apoptotic pathways by linker histones.
Snippet: 3′;H1.2 TL -EGFP reverse, 5′'-CTCAAGCTTAACCTTGGGCTTGGCTTC-3′;H1.2 ΔNTD / H1.2 CTD -EGFP reverse, 5′-CGGGATCCTTTCTTCTTGGGCG-3′. Recombinant Bak constructs were made by PCR amplifying Bak ORF (Origene) and ligating into fluorophore ... Cite SaveSaving
Sorafenib sensitizes (-)-gossypol-induced growth suppression in androgen-independent prostate cancer cells via Mcl-1 inhibition and Bak activation
Lian, Ni, Dai et al
Mol Cancer Ther (2012) 11 (2), 416-26 DOI: 10.1158/1535-7163.MCT-11-0559
AI Summary: OriGene products contributed to this study by providing the Bak shRNA (OriGene, Cat# TG312386) used to knock down Bak expression and the Bak antibody (OriGene, Cat# TA310035) for protein detection, enabling the researchers to investigate the role of Bak in (-)-gossypol and sorafenib-induced cell death in prostate cancer cells.
Showing 1-3 of 3 papers.
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