DGKA (NM_001345) Human Untagged Clone

SKU SC119317

DGKA (untagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: DGKA
• Synonyms: DAGK; DAGK1; DGK-alpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119317 sequence for NM_001345 edited (data generated by NextGen Sequencing); ATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATACATG; GAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGATGGCT; AAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAATCTAT; CTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGAGACT; GGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGTGGTGTGTCTCAATGATGTTTCC; TGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCTTCAAG; CTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTATCCTA; CAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGATTCTT; CAGGAGATGATGAAAGAGATTGACTATGATGGCAGTGGCTCTGTCTCTCAAGCTGAGTGG; GTCCGGGCTGGGGCCACCACCGTGCCACTGCTAGTGCTGCTGGGTCTGGAGATGACTCTG; AAGGACGACGGACAGCACATGTGGAGGCCCAAGAGGTTCCCCAGACCAGTCTACTGCAAT; CTGTGCGAGTCAAGCATTGGTCTTGGCAAACAGGGACTGAGCTGTAACCTCTGTAAGTAC; ACTGTTCACGACCAGTGTGCCATGAAAGCCCTGCCTTGTGAAGTCAGCACCTATGCCAAG; TCTCGGAAGGACATTGGTGTCCAATCACATGTGTGGGTGCGAGGAGGCTGTGAGTCCGGG; CGCTGCGACCGCTGTCAGAAAAAGATCCGGATCTACCACAGTCTGACCGGGCTGCATTGT; GTATGGTGCCACCTAGAGATCCACGATGACTGCCTGCAAGCGGTGGGCCATGAGTGTGAC; NNNNNNCTGCTCCGGGATCACATCCTGCCTCCATCTTCCATCTATCCCAGTGTCCTGGCC; TCTGGACCGGATCGTAAAAATAGCAAAACAAGCCAGAAGACCATGGATGATTTAAATTTG; AGCACCTCTGAGGCTCTGCGGATTGACCCTGTTCCTAACACCCACCCACTTCTCGTCTTT; GTCAATCCTAAGAGTGGCGGGAAGCAGGGNNNNAGGGTGCTCTGGAAGTTCCAGTATATA; TTAAACCCTCGACAGGTGTTCAACCTCCTAAAGGATGGTCCTGAGATAGGGCTCCGATTA; TTCAAGGATGTTCCTGATAGCCGGATTTTGGTGTGTGGTGGAGACGGCACAGTAGGCTGG; ATTCTAGAGACCATTGACAAAGCTAACTTGCCAGTTTTGCCTCCTGTTGCTGTGTTGCCC; CTGGGTACTGGAAATGATCTGGCTCGATGCCTAAGATGGGGAGGAGGTTATGAAGGACAG; AATCTGGCAAAGATCCTCAAGGATTTAGAGATGAGTAAAGTGGTACATATGGATCGATGG; TCTGTGGAGGTGATACCTCAACAAACTGAAGAAAAAAGTGACCCAGTCCCCTTTCAAATC; ATCAATAACTACTTCTCTATTGGCGTGGATGCCTCTATTGCTCATCGATTCCACATCATG; CGAGAGAAATATCCGGAGAAGTTCAACAGCAGAATGAAGAACAAGCTATGGTACTTCGAA; TTTGCCACATCTGAATCCATCTTCTCAACATGCAAAAAGCTGGAGGAGTCTTTGACAGTT; GAGATCTGTGGGAAACCGCTGGATCTGAGCAACCTGTCCCTAGAAGGCATCGCAGTGCTA; AACATCCCTAGCATGCATGGTGGCTCCAACCTCTGGGGTGATACCAGGAGACCCCATGGG; GATATCTATGGGATCAACCAGGCCTTAGGTGCTACAGCTAAAGTCATCACCGACCCTGAT; ATCCTGAAAACCTGTGTACCAGACCTAAGTGACAAGAGACTGGAAGTGGTTGGGCTGGAG; GGTGCAATTGAGATGGGCCAAATCTATACCAAGCTCAAGAATGCTGGACGTCGGCTGGCC; AAGTGCTCTGAGATCACCTTCCACACCACAAAAACCCTTCCCATGCAAATTGACGGAGAA; CCCTGGATGCAGACGCCCTGTACAATCAAGATCACCCACAAGAACCAGATGCCCATGCTC; ATGGGCCCACCCCCCCGCTCCACCAATTTCTTTGGCTTCTTGAGCTAA; ; Clone variation with respect to NM_001345.4; 961 t=>n;962 g=>n;963 t=>n;964 g=>n;965 g=>n;966 g=>n;1170 g=>n;1171 c=>n;1172 a=>n;1173 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001345 unedited; GTAATTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGAGTGAGTCCC; TAGGCCTCCATCTCTCTCCCTTGCTGTACCACCTTCACCACCATCCATGCGACCCCAAGA; GCCTTAATGACTCTAGAAGAGACTCCAGGCAGGGGAAGCTGAAAGGACCTTTCACTCCCT; ACTTTTGGCCAGGGCCTTCTGTGCCACCTGCCAAGACCAGCAGGCCTACCCTCTGAAGAG; GTCCAAGCAACGGAAGTACTACTACGAAGCTGCCTTTCTGGCCATCCTTGAGAAAAATAG; ACAGATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATA; CATGGAATACTCCACCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGAT; GGCTAAATATGTCCAAGGAGATGCCATTGGGTACGAGGGATTCCAGCAATTCCTGAAAAT; CTATCTCGAAGTGGATAATGTTCCCAGACACCTAAGCCTGGCACTGTTTCAATCCTTTGA; GACTGGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGGTGGTGTGTCTCAATGATG; TTTCCTGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATTCACCT; TCAAGCTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTA; TCCTACAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGA; TTCTTCAGGAGATGATGAAGAGATGCTATGATGGCAGGGGCTCTGTTCTCAACTGGAGGG; GTCCGGGCTGGGGCCACACCGTGCCCTGCTATGCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001345 unedited; GGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGATCCCAAGGGGGAAACA; CAAGTCATTTTATTCAGATGAGGCACTTTCAATGTATGTGTTTTGGGGTGTGTGTATGTG; TGTGCGCATGGGGAACAGTGAGGTGGTGCAGGATAATAAATACTGTGAGGGTGAAGGAAC; ACTCCCCCAAGCTGGCAGGAGTATGTGGCAGCAATGTACAGAGCCTCGGGAGTAGAGTCC; AGGGACAGGGAGGTGGGTTCAAGGCTGGCTTGGAGGCCAAGGGTGTCCCCCTTAGCTCAA; GAAGCCAAAAAAATTGGTGGAGCGGGGGGGTGGGCCCATGAGCATGGGCATCTGGTTCTT; GTGGGTGATCTTGATTGTACAGGGCGTCTGCATCCAGGGTTCTCCGTCAATTTGCATGGG; AAGGGTTTTTGTGGTGTGGAAGGTGATCTCAGAGCACTTGGCCAGCCGACGTCCAGCATT; CTTGAGCTTGGTATAGATTTGGCCCATCTCAATTGCACCCTCCAGCCCAACCACTTCCAG; TCTTTTGTTACTTACGTCTGGTACACAGGTTTTCAGGATATCACGGTCGGTGATGACTTT; AACTGAAGCACCTAACGGCTGGTTGATCCCATATATATCCCCCATGGCGCGCTCCTGGTA; TCACCTCCTAAACGCCGGAGCCCCCATGCGATTGTTAGGGATGGTTACGACCTGCGAACG; CCTCTCCAGGGACAGGGTGCCCAAACCCCCCGGTTTCCCCACACATCTCCCTCCCCCAAC; ACTTCCTTCGTTCTTTTATATGTCGGAAACGAACCCTGTTACCATCCCCAAACANGCGAA; GACATANATAGTGTTCTTTATTTCCTTCCTCTATCTTTGTGGGAAATCGGTGTGGAATTG; GGGGAGAGAGGGGCGCCCCGGCCGCGCCCTCCCATGACATTTCACTCCACCTTCTTCCGC; CCCCCCCATTCGCCCTCCGTCTTCCCCTCCGCCCTCCCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001345
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001345.4, NP_001336.2
• RefSeq Size: 2756 bp
• RefSeq ORF: 2208 bp
• Locus ID: 1606
• UniProt ID: P23743
• Cytogenetics: 12q13.2
• Domains: DAGKa, DAGKc, DAG_PE-bind, EFh
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Summary: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]; Transcript Variant: This variant (3), as well as variants 1, 2, and 4-6, encodes isoform a.