DHPS (NM_001930) Human Untagged Clone

SKU SC118951

DHPS (untagged)-Human deoxyhypusine synthase (DHPS), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DHPS
• Synonyms: DHS; DS; MIG13; NEDSSWI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118951 sequence for NM_001930 edited (data generated by NextGen Sequencing); ATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCAC; AGCTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTG; AATTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGG; CGCGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTGGAACCACTGTCACAGGAT; GAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTTACCAGCTGCACCATTTTC; CTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCGCTACCTTGTG; CAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATC; AAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAGCTCCGGGAG; AACGGGATCAATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGCAAGTTTGAG; GACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCAGAACACAGAGGGTGTAAAG; TGGACGCCTTCTAAGATGATCGCCCGGCTGGGCAAGGAGATCAACAACCCAGAGTCCGTG; TATTACTGGGCCCAGAAGAACCACATCCCTGTGTTTAGTCCCGCACTTACAGACGGCTCG; CTGGGCGACATGATCTTCTTCCATTCCTACAAGAACCCGGGCCTGGTCCTGGACATCGTT; GAGGACCTGAGGCTCATCAACACACAGGCCATCTTTGCCAAGTGCACTGGGATGATCATT; CTGGGCGGGGGCGTGGTCAAGCACCACATTGCCAATGCCAACCTCATGCGGAACGGGGCC; GACTACGCTGTTTACATCAACACAGCCCAGGAGTTTGATGGCTCTGACTCAGGTGCCCGA; CCAGACGAGGCTGTCTCCTGGGGCAAGATCCGGGTGGATGCACAGCCCGTCAAGGTCTAT; GCTGACGCCTCCCTGGTCTTCCCCCTGCTTGTGGCTGAAACCTTTGCCCAGAAGATGGAT; GCCTTCATGCATGAGAAGAACGAGGACTGA; ; Clone variation with respect to NM_001930.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001930 unedited; GCAAAAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGAGACGCGCGC; GTCGGGGTTTAACGCGTTTCTGGGCCGCCGTAAGCCCGGCCTAGGGGCAGCTTTGACTCG; AGAGCCGGCTATAGGCGCATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTG; GCCGCCGTGCTAAAGCACAGCTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTAC; GACTTCAACCGCGGTGTGAATTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTC; CAAGCAACCAACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTG; GAACCACTGTCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTT; ACCAGCTGCACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAG; ACCATTCGCTACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGC; GTGGAGGAAGACCTCATCAAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGG; GGGAAGGAGCTCCGGGAGAACGGGATCAATAGGATCGGAACCCTGGCTGGTGCCCAATGA; GAATTACTGCCAGTTTGAGGACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCA; GAACACAGAGGGTGTAAAGTGGACGCCTTCTAAGATGATCGCCGGCTGGGCAAGGAGATC; AACACCCCAGAGTCGTGTATTACTGGGCCCCAGAGGACCACATCCCTGTGGTTAGTCCCG; CACTTACAGACAGCTCGCTGGGCGACATGAACTTCTTCCATTCCTACCAGAACCCNGGGC; TGTTCCTGGACATCGTTGAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001930 unedited; GGGGGGATGGCCCACNCNANNNTNTTCTTACACAAGAATTTGGCTTACACTACCANCTTT; ATCTANACCGTAGCTGACCAAAAAGTAGGGAGGGGCTGGGTCTGCAAATTAATAAATAGA; AGAGGGGGTAAGACCTTCCTGGGACCGCAGCCGCTCAGTCCTCGTTCTTCTCATGCATGA; AGGCATCCATCTTCTGGGCAAAGGTTTCAGCCACAAGCAGGGGGAAGACCAGGGAGGCGT; CAGCATAGACCTTGACGGGCTGTGCATCCACCCGGATCTTGCCCCAGGAGACAGCCTCGT; CTGGTCGGGCACCTGAGTCAGAGCCATCAAACTCCTGGGCTGTGTTGATGTAAACAGCGT; AGTCGGCCCCGTTCCGCATGAGGTTGGCATTGGCAATGTGGTGCTTGACCACGCCCCCGC; CCAGAATGATCATCCCAGTGCACTTGGCAAAGATGGCCTGTGTGTTGATGAGCCTCAGGT; CCTCAACGATGTCCAGGACCAGGCCCGGGTTCTTGTAGGAATGGAAGAAGATCATGTCGC; CCAGCGAGCCGTCTGTAAGTGCGGGACTAAACACAGGGATGTGGTTCTTCTGGGCCCAGT; AATACACGGACTCTGGGTTGTTGATCTCCTTGCCCAGCCGGGCGATCATCTTAGAAGGCG; TCCACTTTACACCCTCTGTGTTCTGCTCCATCACCATCTGGTCCAGAATGGGCATCAGCC; AGTCCTCAAACTTGCAGTAATTCTCATTGGGCACCAGCANGTTTCCGATCCTATTGATCC; CGTTCTCCGGNAGCTNCTTTCCCCCTGAGCTAAACTCGCCCAAGTATGTGGGGCGCCAGG; CACTTGATGAGGTCTTNCTNCACGCCGNCAGCTTGGGTCACCATTACGTCCACCCATGTT; GTGCTGCACAAGGTANCGAATGGTCTCACGGATGCCCTGACTGAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001930
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001930.2, NP_001921.1
• RefSeq Size: 1351 bp
• RefSeq ORF: 1110 bp
• Locus ID: 1725
• UniProt ID: P49366
• Cytogenetics: 19p13.13
• Domains: DS
• Summary: This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).