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NDUFS8 (NM_002496) Human Untagged Clone

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SKU
SC118623
NDUFS8 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NDUFS8
Synonyms CI-23k; CI23KD; MC1DN2; TYKY
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118623 sequence for NM_002496 edited (data generated by NextGen Sequencing)
ATGCGCTGCCTGACCACGCCTATGCTGCTGCGGGCCCTGGCCCAGGCTGCACGTGCAGGA
CCTCCTGGTGGCCGGAGCCTCCACAGCAGTGCAGTGGCAGCCACCTACAAGTATGTGAAC
ATGCAGGATCCCGAGATGGACATGAAGTCAGTGACTGACCGGGCAGCCCGCACCCTGCTG
TGGACTGAGCTCTTCCGAGGCCTGGGCATGACCCTGAGCTACCTGTTCCGGGAACCGGCC
ACCATCAACTACCCGTTCGAGAAGGGCCCGCTGAGCCCTCGCTTCCGTGGGGAGCATGCG
CTGCGCCGGTACCCATCCGGGGAGGAGCGTTGCATTGCCTGCAAGCTCTGCGAGGCCATC
TGCCCCGCCCAGGCCATCACCATCGAGGCTGAGCCAAGAGCTGATGGCAGCCGCCGGACC
ACCCGCTATGACATCGACATGACCAAGTGCATCTACTGCGGCTTCTGCCAGGAGGCCTGT
CCCGTGGATGCCATCGTCGAGGGCCCCAACTTTGAGTTCTCCACGGAGACCCATGAGGAG
CTGCTGTACAACAAGGAGAAGTTGCTCAACAACGGGGACAAGTGGGAGGCCGAGATCGCC
GCCAACATCCAGGCTGACTACTTGTATCGGTGA

Clone variation with respect to NM_002496.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002496 unedited
AGGTCGAATTTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACGAGGAGCCTCCG
CCTCCCGATTGACTGGCCTGCTTGGCAAGGCAAGTAGCGGCGGCGCTTCAAGATGCGCTG
CCTGACCACGCCTATGCTGCTGCGGGCCCTGGCCCAGGCTGCACGTGCAGGACCTCCTGG
TGGCCGGAGCCTCCACAGCAGTGCAGTGGCAGCCACCTACAAGTATGTGAACATGCAGGA
TCCCGAGATGGACATGAAGTCAGTGACTGACCGGGCAGCCCGCACCCTGCTGTGGACTGA
GCTCTTCCGAGGCCTGGGCATGACCCTGAGCTACCTGTTCCGGGAACCGGCCACCATCAA
CTACCCGTTCGAGAAGGGCCCGCTGAGCCCTCGCTTCCGTGGGGAGCATGCGCTGCGCCG
GTACCCATCCGGGGAGGAGCGTTGCATTGCCTGCAAGCTCTGCGAGGCCATCTGCCCCGC
CCAGGCCATCACCATCGAGGCTGAGCCAAGAGCTGATGGCAGCCGCCGGACCACCCGCTA
TGACATCGACATGACCAAGTGCATCTACTGCGGCTTCTGCCAGGAGGCCTGTCCCGTGGA
TGCCATCGTCGAGGGCCCCAACTTTGAGTTCTCCACGGAGACCCATGAGGAGCTGCTGTA
CAACAAGGAGAAGTTGCTCAACAACGGNGACAAGTGGGAGGCCGAGATCGCCGCCAACAT
CCAGGCTGACTACTTGTATCGGTGACGCCCCACCGGNNCCCGCAGCCCTGCTGCCCAATA
AAACCACTCCGACCCCCANAAANANANNAANNNNNNAACAAACAAAAAAAACAANNNNAC
AAAAAAANAAAAAAAAACACAANAAAAAAAAAAAAACCCCCTTTTAAATGCGGCCGGGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002496 unedited
GCCCGCGCCGGCCCCGCCGGCCCNCCCGCCCCCCGGTTTTTCGGCGTGGTTTTTTGGGCA
GCAGGGCTGCGGGCCGGTGGGGCGTCACCGCTACAAGTAGTCAGCCTGGCTGTTGGCGGC
GATCTCGGCCTCCCACTTGTCCCCGTTGTTGAGCAACTTCTCCTTGTTGTACAGCAGCTC
CTCATGGGTCTCCGTGGAGAACTAAATTGGGGCCCTCGACGATGGCATCCACGGGACAGG
CCTCCTGGCAGAAGCCGCAGTAGATGCACTTGGTCATGTCGATGTCATAGCGGGTGGTCC
GGCGGCTGCCATCAGCTCTTGGCTCAGCCTCGATGGTGATGGCCTGCGCGGGGCAGATGG
CCTCGCAGAGCTTGCAGGCAATGCAACGCTCCTCCCCGGATGGGTACCGGCGCAGCGCAT
GCTCCCCACGGAAGCGAGGGCTCATCGGGCCCTTCTCGAACGGGTATTTGATGGTGGCCG
GTTCCCGGAACAGGTAGCTCAGGGTCATGCCCACGCCTCTGAAGAGCTCAGTCCACAGCA
GGGTGCGGGCTGCCCGGTCAGTCACTGACTTCCTGTCCATTTTCGGATCCTGCATTGTTC
ACATACTTGTTACGTGGCTGTCACTGCACTGCTGCCGAGGCTTCTGCCCACCCATGAGGT
TCCTGCACGTGCACCCTGGGTCCCCTGCCCCCACCCTCCTACGTCTTGTATATGCCTCGC
ATCTTGCAAACGCATCCCCTCCTTCGCCTGCCTACCTCGGCCATTCTATCTGTATTGCCG
ATGCTTCCTTTTTGCCAAATCCTCTGTCGCCCGTTTCTGGACTTGGTTTACAAAATTTTT
TACTGGTTATACTAATTTATCTTCTATCTTTATTACACCTAACTACCTTATAAGTAGAAC
CCTCCATCTTTCCCCT
Restriction Sites NotI-NotI |
ACCN NM_002496
Insert Size 850 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002496.1, NP_002487.1
RefSeq Size 779 bp
RefSeq ORF 633 bp
Locus ID 4728
UniProt ID O00217
Cytogenetics 11q13.2
Domains fer4
Protein Pathways Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Summary This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_002496" in other vectors (6)
SKU Description Size Price
RC216036 NDUFS8 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein 10 ug
$300.00
RC216036L1 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC216036L2 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RC216036L3 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC216036L4 Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RG216036 NDUFS8 (tGFP-tagged) - Human NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) (NDUFS8), nuclear gene encoding mitochondrial protein 10 ug
$489.00 $500.00

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