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NFYA (NM_002505) Human Untagged Clone

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SKU
SC118595
NFYA (untagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NFYA
Synonyms CBF-A; CBF-B; HAP2; NF-YA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118595 sequence for NM_002505 edited (data generated by NextGen Sequencing)
ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGA
CAGATTCAGCAGCAGCAGCAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGCCCAG
GTGGCATCCGCCTCAGGCCAGCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCCATTA
ATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCATCATGGTCCAG
GCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAGGGT
TTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTG
CAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCT
GTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCA
CAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTC
CAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGCAGGA
GCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGGGCAAGGGACTGTC
ACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGATGGTT
CCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGATGCTT
GAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAGGCAA
GCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCTGCAT
GAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGGACGATTTTTCTCT
CCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGATGAAGAAGCAATG
ACACAGATCATCCGAGTGTCCTAA

Clone variation with respect to NM_002505.4
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002505 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCG
GCACGAGGCGGCGGCAGCGGCGGCTGGAGCCTCTGATTGGGTTTCGGAGTCCGGTACTGG
AGCCAATCAGCGCGGGCAGCGAACCGGGGGAGCGAGGCACGGAGTGTACCTCACAGCCTT
CTAGGATCTCCAGAGTGGACAGGAATCTCACTTGGAGGGACCATGGAGCAGTATACAGCA
AACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGCAGCAGCAG
CAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGCCCAGGTGGCATCCGCCTCAGGC
CAGCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGA
GGCCAGCTAATCACATCAACTGGCCAACCCATCATGGTCCAGGCTGTCCCTGGTGGACAA
GGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAGGGTTTGCAGCAAATACAGTTG
GTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGCAGGGCCAGCAG
GGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGACT
CAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAG
ACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAGTTACAGTCCCTG
GTTCAGGCATGATCACTATCCAGCAGCCAGTTTGGGCAGAGCCCAGATTGTNAAACAGGA
GCCATACCACACACCAGCAGTGGCAAGGGACTGTACTGTGCACTACAGTG
Restriction Sites NotI-NotI |
ACCN NM_002505
Insert Size 3800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002505.3, NP_002496.1
RefSeq Size 2834 bp
RefSeq ORF 1044 bp
Locus ID 4800
UniProt ID P23511
Cytogenetics 6p21.1
Domains CBF
Protein Families Transcription Factors
Protein Pathways Antigen processing and presentation
Summary The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) includes alternate exon B, resulting in the long isoform, isoform 1.
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Documents
Resources
"NM_002505" in other vectors (6)
SKU Description Size Price
RC213263 NFYA (Myc-DDK-tagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1 10 ug
$457.00
RC213263L1 Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC213263L2 Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, mGFP tagged 10 ug
$757.00
RC213263L3 Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC213263L4 Lenti ORF clone of Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1, mGFP tagged 10 ug
$757.00
RG213263 NFYA (tGFP-tagged) - Human nuclear transcription factor Y, alpha (NFYA), transcript variant 1 10 ug
$489.00 $657.00

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