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Serglycin (SRGN) (NM_002727) Human Untagged Clone

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SKU
SC118445
SRGN (untagged)-Human serglycin (SRGN), transcript variant 1
  $225.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Serglycin
Synonyms PPG; PRG; PRG1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118445 sequence for NM_002727 edited (data generated by NextGen Sequencing)
ATGATGCAGAAGCTACTCAAATGCAGTCGGCTTGTCCTGGCTCTTGCCCTCATCCTGGTT
CTGGAATCCTCAGTTCAAGGTTATCCTACGCAGAGAGCCAGGTACCAATGGGTGCGCTGC
AATCCAGACAGTAATTCTGCAAACTGCCTTGAAGAAAAAGGACCAATGTTCGAACTACTT
CCAGGTGAATCCAACAAGATCCCCCGTCTGAGGACTGACCTTTTTCCAAAGACGAGAATC
CAGGACTTGAATCGTATCTTCCCACTTTCTGAGGACTACTCTGGATCAGGCTTCGGCTCC
GGCTCCGGCTCTGGATCAGGATCTGGGAGTGGCTTCCTAACGGAAATGGAACAGGATTAC
CAACTAGTAGACGAAAGTGATGCTTTCCATGACAACCTTAGGTCTCTTGACAGGAATCTG
CCCTCAGACAGCCAGGACTTGGGTCAACATGGATTAGAAGAGGATTTTATGTTATAA

Clone variation with respect to NM_002727.2
92 g=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002727 unedited
AATTGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGAGTTGGC
GTGCAGCTGGGAGAGCTAGACTAAGTTGGTCATGATGCAGAAGCTACTCAAATGCAGTCG
GCTTGTCCTGGCTCTTGCCCTCATCCTGGTTCTGGAATCCTCAGTTCAAGGTTATCCTAC
GCAGAGAGCCAGGTACCAATGGGTGCGCTGCAATCCAGACAGTAATTCTGCAAACTGCCT
TGAAGAAAAAGGACCAATGTTCGAACTACTTCCAGGTGAATCCAACAAGATCCCCCGTCT
GAGGACTGACCTTTTTCCAAAGACGAGAATCCAGGACTTGAATCGTATCTTCCCACTTTC
TGAGGACTACTCTGGATCAGGCTTCGGCTCCGGCTCCGGCTCTGGATCAGGATCTGGGAG
TGGCTTCCTAACGGAAATGGAACAGGATTACCAACTAGTAGACGAAAGTGATGCTTTCCA
TGACAACCTTAGGTCTCTTGACAGGAATCTGCCCTCAGACAGCCAGGACTTGGGTCAACA
TGGATTAGAAGAGGATTTTATGTTATAAAAGAGGATTTTCCCACCTTGACACCAGGCAAT
GTAGTTAGCATATTTTATGTACCATGGTTATATGATTAATCTTGGGACAAAGAATTTTAT
AGAAATTTTTAAACATCTGAAAAAGAAGCTTAAGTTTTATCATCCTTTTTTTTCTCATGA
ATTCTTAAAGGATTATGCTTTAATGCTGNTATCTATCTTATTGTTCTTGAAATACCTGCA
TTTTTTGGTATCATGTTCAACCAACATCATTATGAAATTAATTAGATTCCCATGGCCATA
AAATGGCTTTAAGAAATATATATATTTTTAAAGTAGCTTGAAAGCCAAATGGCAGGTATA
TTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002727 unedited
TTGCTGCTTNTATATATAGTAACATTTGGAAATACACANATATCCAGGNAAAAAAGGTAA
AGCAGTATGAGGTATTGACAGCACAACTGTGTTTTAATTTGGGAGTTAATTTGGGGTGTT
ATGTAACAGCAATCATACTTGTAGACAAACACATCCACTAGCTCTGCAAACACATTCAAA
GGTAATAGTATATAGTACACTCCACCCTGTAAAATATGTCAAATACCCCCACTCTACAAA
TTCACTGTGTTTCATTATTTTTTTTTTGTTTTTATAAGAAAAGGGGCATATCATTATAAT
TCACAATCCAAGTCAGAGTCTTAATTTAGGTATGAAATATTACCTGCCAATTTGCTTCTC
AAGCTACTTTAAAAATATATATATATTCTTTAAAGCCATTTTATGGCCATGGGAATCTAA
TTAATTTCATAATGATGTTGGTTGAACATGATACCAAAAAATGCAGGTATTTTCAAGAAC
AATAAGATAGATAACAGCATTAAAGCATAATCCTTTAAGAATTCATGAGAAAAAAAAGGA
TGATAAAACTTAAGCTTCTTTTTCAGATGTTTAAAAATTTCTATAAAATTCTTTGTCCCA
AGATTAATCATATAACCATGGTACATAAAATATGCTAACTACATTGCCTGGTGTCAAGGT
GGGAAAATCCTCTTTTATAACATAAAATCCTCTTCTAATCCATGTTGACCCAAGTCCTGG
CTGTCTGNAGGCAGATTCCTGTCAAGAGACCTAAGGTTGTCATGGAAAGCATCACTTTCG
GCTACTAGNTGGTAATCCTGTTCCATTTTCGTTAGGAGCCACTCCCAGTCCTGACCANAG
CCGAGCCGGAGCCGAAGCTGATCANNAGTAGTCTCAGAAGTGGGAAGATCGATTCAAGTC
TGGATTTCGGCTTGGAAAAGGCAGTCCTCAACGGGGAACTGTTGGATCACTGNAAGAGTC
GACATGGCCTTTTCTTAAGGGA
Restriction Sites NotI-NotI |
ACCN NM_002727
Insert Size 1270 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002727.2, NP_002718.2
RefSeq Size 1270 bp
RefSeq ORF 477 bp
Locus ID 5552
UniProt ID P10124
Cytogenetics 10q22.1
Domains Serglycin
Summary This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. provided by RefSeq, Jul 2010
Transcript Variant: This variant (1) lacks an exon in the 5' UTR compared to variant 2. Both variants 1 and 3 encode isoform 1.
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Documents
Resources
"NM_002727" in other vectors (6)
SKU Description Size Price
RC202118 SRGN (Myc-DDK-tagged)-Human serglycin (SRGN), transcript variant 1 10 ug
$225.00
RC202118L1 Lenti ORF clone of Human serglycin (SRGN), transcript variant 1, Myc-DDK-tagged 10 ug
$525.00
RC202118L2 Lenti ORF clone of Human serglycin (SRGN), transcript variant 1, mGFP tagged 10 ug
$525.00
RC202118L3 Lenti ORF clone of Human serglycin (SRGN), transcript variant 1, Myc-DDK-tagged 10 ug
$525.00
RC202118L4 Lenti ORF clone of Human serglycin (SRGN), transcript variant 1, mGFP tagged 10 ug
$525.00
RG202118 SRGN (tGFP-tagged) - Human serglycin (SRGN), transcript variant 1 10 ug
$489.00

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