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PSMB3 (NM_002795) Human Untagged Clone

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SKU
SC118416
PSMB3 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PSMB3
Synonyms HC10-II
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118416 sequence for NM_002795 edited (data generated by NextGen Sequencing)
ATGTCTATTATGTCCTATAACGGAGGGGCCGTCATGGCCATGAAGGGGAAGAACTGTGTG
GCCATCGCTGCAGACAGGCGCTTCGGGATCCAGGCCCAGTTGGTGACCACGGACTTCCAG
AAGATCTTTCCCATGGGTGACCGGCTGTACATCGGTCTGGCCGGGCTCGCCACTGACGTC
CAGACAGTTGCCCAGCGCCTCAAGTTCCGGCTGAACCTGTATGAGTTGAAGGAAGGTCGG
CAGATCAAACCTTATACCCTCATGAGCATGGTGGCCAACCTCTTGTATGAGAAACGGTTT
GGCCCTTACTACACTGAGCCAGTCATTGCCGGGTTGGACCCGAAGACCTTTAAGCCCTTC
ATTTGCTCTCTAGACCTCATCGGCTGCCCCATGGTGACTGATGACTTTGTGGTCAGTGGC
ACCTGCGCCGAACAAATGTACGGAATGTGTGAGTCCCTCTGGGAGCCCAACATGGATCCG
GATCACCTGTTTGAAACCATCTCCCAAGCCATGCTGAATGCTGTGGACCGGGATGCAGTG
TCAGGCATGGGAGTCATTGTCCACATCATCGAGAAGGACAAAATCACCACCAGGACACTG
AAGGCCCGAATGGACTAA

Clone variation with respect to NM_002795.2
100 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002795 unedited
GGTCAGGAATAGTATCCGATTCATATAGGCGGCCGCGCAATTCGCACGAGGGCTAGACCC
GGTTTACTGGAATTGCTCTGGCGATCGAGGGGTCCTAGTACACCGCAATCATGTCTATTA
TGTCCTATAACGGAGGGGCCGTCATGGCCATGAAGGGGAAGAACTGTGTGGCCATCGCTG
CAGACAGGCGCTTCGGGATCCAGGCCCTGTTGGTGACCACGGACTTCCAGAAGATCTTTC
CCATGGGTGACCGGCTGTACATCGGTCTGGCCGGGCTCGCCACTGACGTCCAGACAGTTG
CCCAGCGCCTCAAGTTCCGGCTGAACCTGTATGAGTTGAAGGAAGGTCGGCAGATCAAAC
CTTATACCCTCATGAGCATGGTGGCCAACCTCTTGTATGAGAAACGGTTTGGCCCTTACT
ACACTGAGCCAGTCATTGCCGGGTTGGACCCGAAGACCTTTAAGCCCTTCATTTGCTCTC
TAGACCTCATCGGCTGCCCCATGGTGACTGATGACTTTGTGGTCAGTGGCACCTGCGCCG
AACAAATGTACGGAATGTGTGAGTCCCTCTGGGAGCCCAACATGGATCCGGATCACCTGT
TTGAAACCATCTCCCAAGCCATGCTGAATGCTGTGGACCGGGATGCAGTGTCAGGCATGG
GAGTCATTGTCCACATCATCGAGAAAGACAAAATCACCACCAGGACACTGAAGGCCCGAA
TGGACTAACCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTCTTGACATAAAATAGCCTT
GTTTTTCAAAAAACAAAAAAAAAACTCGACTTTAGATTGCGGCCGCGGCCATACCTGTTT
CCTGAACAGAACCCCGGTGGGATCCCTGTGACCCCTCCCATGGCCTTTCCTGCCCCTGGA
AATTGCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002795 unedited
TAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAAGACAGGCTATTTTATTTCAAAAAAAGAA
AAAAAAGTGGGCCTCTGGGAACAGGGTTAGTCCATTCGGGCCTTCAGTGTCCTGGTGGTG
ATTTTGTCCTTCTCGATGATGTGGACAATGACTCCCATGCCTGACACTGCATCCCGGTCC
ACAGCATTCAGCATGGCTTGGGAGATGGTTTCAAACAGGTGATCCGGATCCATGTTGGGC
TCCCAGAGGGACTCACACATTCCGTACATTTGTTCGGCGCAGGTGCCACTGACCACAAAG
TCATCAGTCACCATGGGGCAGCCGATGAGGTCTAGAGAGCAAATGAAGGGCTTAAAGGTC
TTCGGGTCCAACCCGGCAATGACTGGCTCAGTGTAGTAAGGGCCAAACCGTTTCTCATAC
AAGAGGTTGGCCACCATGCTCATGAGGGTATAAGGTTTGATCTGCCGACCTTCCTTCAAC
TCATACAGGTTCAGCCGGAACTTGANGCGCNTGGGCACTGTCTGGACGTCAGTGGCGAAG
CCCGCCAGACCGATGTACAGCCGGTCACCCCATGGAAAGATCTTCTGGAGTCCGTGGTCC
CCAACTGGGCCTGAATCCCGAACCCCTGTCTGCAACAAGGCCACCAGTTCTTCCCCTTCA
TGCCCTGACGGCCCTCCGTTATGGACATAAAAAACTGAATGGGGGGTCCTGACCCCCCAA
TCCCCAAAAAATTCCCGTAAACGGGTTTACCCCCTTGGCCAATATCGGGCCGCCCTAAGG
GGGCCCTATCCAAATTTTACGCGGTCAAAACACACCTTGTTTTATTACCCCCCCCCCGAC
CCCCCCCCCCCTTTTTGTACCCGGGGGGGGTTTTACCCATTTTTAAAAACCCCCCTTTTT
TGGCCCAAACCCCCCCT
Restriction Sites NotI-NotI |
ACCN NM_002795
Insert Size 780 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002795.2, NP_002786.2
RefSeq Size 784 bp
RefSeq ORF 618 bp
Locus ID 5691
UniProt ID P49720
Cytogenetics 17q12
Domains proteasome
Protein Families Druggable Genome, Protease
Protein Pathways Proteasome
Summary The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants provided by RefSeq, Sep 2013
Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein.
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"NM_002795" in other vectors (5)
SKU Description Size Price
RC201296 PSMB3 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3) 10 ug
$300.00
RC201296L3 Lenti ORF clone of Human proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3), Myc-DDK-tagged 10 ug
$600.00
RC201296L4 Lenti ORF clone of Human proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3), mGFP tagged 10 ug
$600.00
RG201296 PSMB3 (tGFP-tagged) - Human proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3) 10 ug
$489.00 $500.00
SC324434 PSMB3 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3) 10 ug
$300.00

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